Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 967,816 G→T 15.2% A190D (GCT→GAT)  ydeV ← predicted sugar kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE967,8160GT15.2% 224.7 / 16.5 72A190D (GCT→GAT) ydeVpredicted sugar kinase
Reads supporting (aligned to +/- strand):  ref base G (25/36);  new base T (5/6);  total (30/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

TAGGCCAGCCATATCCAGCAATGCAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCAGCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCATATAGGCCAGCCAGTCGCTGATCATGGTGA  >  minE/967746‑967882
                                                                      |                                                                  
tAGGCCAGCCATATCCAGCAATGCAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCAg                                                                    <  1:3549451/71‑1 (MQ=255)
tAGGCCAGCCATATCCAGCAATGCAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCAg                                                                    <  1:2215602/71‑1 (MQ=255)
 aGGCCAGCCATATCCAGCAATGCAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCAg                                                                    <  1:3598524/70‑1 (MQ=255)
     cagccaTATCCAGCAATGCAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTa                                                               <  1:204236/71‑1 (MQ=255)
       gccaTATCCAGCAATGCAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCAGCGTTaga                                                             <  1:3980679/71‑1 (MQ=255)
       gccaTATCCAGCAATGCAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCAGCGTTa                                                               >  1:3113771/1‑69 (MQ=255)
          atatCCAGCAATGCAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCAGCGTTAGAgg                                                           >  1:340380/1‑70 (MQ=255)
          atatCCAGCAATGCAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCAGCGTTAGAgg                                                           >  1:3569342/1‑70 (MQ=255)
           tatCCAGCAATGCAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCAGCGTTa                                                               <  1:1649235/65‑1 (MQ=255)
           tatCCAGCAATGCAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCAGCGTTa                                                               <  1:172198/65‑1 (MQ=255)
             tCCAGCAATGCAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATc                                                       >  1:2317628/1‑71 (MQ=255)
             tCCAGCAATGCAGGTTTCCAGTCACGGGTGGATAGATCAAGAAGTCCAGTGGTGCCAGCGTTAGAGGGATc                                                       >  1:4118154/1‑71 (MQ=255)
               cAGCAATGCAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCAGCGTTAGAGGGATcc                                                      <  1:557659/70‑1 (MQ=255)
                aGCAATGCAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCAGc                                                                   <  1:3356417/56‑1 (MQ=255)
                 gCAATGCAGGTTTCCAGTCACGGGTGGTTAGATCAAGAACTCCCGTGGTGCCAGCGt                                                                 <  1:689630/57‑1 (MQ=255)
                    aTGCAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCAGCGTTAGAGGGATCCACCGcc                                                <  1:312645/71‑1 (MQ=255)
                       cAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCAGCGTTAGAGGGATCCACCGCCAGt                                             >  1:2216933/1‑71 (MQ=255)
                       cAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCAGCGTTAGAGGGATCCACCGCCAGt                                             >  1:3662426/1‑71 (MQ=255)
                         ggTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCAGCGTTAGAGGGATCCACCGCCAGTTc                                           >  1:3768434/1‑71 (MQ=255)
                          gTTTCCAGTCACGGGTTGTTAGATCAAGAAGTCCCGTTGTGCCAGc                                                                   <  1:298192/46‑1 (MQ=255)
                          gTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCAGCGTTa                                                               <  1:2242003/50‑1 (MQ=255)
                            ttCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCAGCGTTAGAGGGATCCACCGCCAGTTcgcc                                        >  1:1020809/1‑71 (MQ=255)
                             tCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCAGCGTTAGAGGGATCCACCGCCAGTTcgccg                                       >  1:3081589/1‑71 (MQ=255)
                                 gTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCt                                     >  1:1302704/1‑69 (MQ=255)
                                 gTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCAGCGTTAGAggg                                                          <  1:3816827/48‑1 (MQ=255)
                                 gTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCAGCGTTAGAGGGATCCACCGCCAGTTCGCCGCt                                     >  1:2850962/1‑69 (MQ=255)
                                  tCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCAGCGTTa                                                               <  1:830377/42‑1 (MQ=255)
                                   cACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCAGCGTTa                                                               >  1:3843624/1‑41 (MQ=255)
                                   cACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCAGCGTTAGAGGGa                                                         >  1:3073756/1‑47 (MQ=255)
                                    aCGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTcgccg                                       <  1:2750497/64‑1 (MQ=255)
                                    aCGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCAGCGTTaga                                                             <  1:199758/42‑1 (MQ=255)
                                    aCGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCAGCGTTa                                                               <  1:226775/40‑1 (MQ=255)
                                    aCGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCAGCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGc                                 <  1:2048085/70‑1 (MQ=255)
                                    aCGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCAGCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCa                                <  1:2576989/71‑1 (MQ=255)
                                    aCGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCAGCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCa                                >  1:1900121/1‑71 (MQ=255)
                                     cGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCAGCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCat                               <  1:1710969/71‑1 (MQ=255)
                                      gggTGGTTAGATTAAGAAGTCCCGTGGTGCCAGCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCata                              <  1:2218877/71‑1 (MQ=255)
                                      gggTGGTTAGATCAAGAAGTCCCGTGGTGCCAGCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCata                              <  1:681749/71‑1 (MQ=255)
                                         tggtTAGATCAAGAAGTCCCGTGGTGCCAGCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCATATAg                           <  1:57629/71‑1 (MQ=255)
                                           gtTAGATCAAGAAGTCCCGTGGTGCCATCGTTagag                                                            >  1:1183362/1‑36 (MQ=255)
                                            ttAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCata                              <  1:3090141/65‑1 (MQ=255)
                                            ttAGATCAAGAAGTCCCGTGGTGCCAGCGTTAGAGGGATCCa                                                     <  1:2707057/42‑1 (MQ=255)
                                              aGATCAAGAAGTCCCGTGGTGCCAGCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCATATAGgccag                      >  1:3244660/1‑71 (MQ=255)
                                                aTCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCa                                <  1:1256537/59‑1 (MQ=255)
                                                aTCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCa                                <  1:1941808/59‑1 (MQ=255)
                                                aTCAAGAAGTCCCGTGGTGCCAGCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCata                              >  1:1088504/1‑61 (MQ=255)
                                                aTCAAGAAGTCCCGTGGTGCCAGCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCata                              <  1:1559402/61‑1 (MQ=255)
                                                aTCAAGAAGTCCCGTGGTGCCAGCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCa                                <  1:3765259/59‑1 (MQ=255)
                                                 tCAAGAAGTCCCGTGGTGCCAGCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCATATAGgccag                      >  1:3606663/1‑68 (MQ=255)
                                                  cAAGAAGTCCCGTGGTGCCAGCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCata                              >  1:3122483/1‑59 (MQ=255)
                                                  cAAGAAGTCCCGTGGTGCCAGCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCATATAGGccagccag                  >  1:1289503/1‑71 (MQ=255)
                                                   aagaagTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCATATAGGCCAGCCAGt                 >  1:183355/1‑71 (MQ=255)
                                                   aagaagTCCCGTGGTGCCAGCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGa                                   >  1:1377141/1‑53 (MQ=255)
                                                   aagaagTCCCGTGGTGCCAGCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCATATAGgccag                      <  1:1837708/66‑1 (MQ=255)
                                                     gaagTCCCGTGGTGCCAGCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCata                              >  1:1250245/1‑56 (MQ=255)
                                                     gaagTCCCGTGGTGCCAGCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCATATAGGCCAGCCAGTCg               <  1:3047121/71‑1 (MQ=255)
                                                     gaagTCCCGTGGTGCCAGCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCATATAGGCCAGCCAGTCg               <  1:2629019/71‑1 (MQ=255)
                                                      aagTCCCGTGGTGCCAGCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCATATAGGCCAGCCAGTCGc              >  1:2561213/1‑71 (MQ=255)
                                                           ccGTGGTGCCAGCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAg                                  <  1:211813/46‑1 (MQ=255)
                                                           ccGTGGTGCCAGCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCata                              >  1:970003/1‑50 (MQ=255)
                                                           ccGTGGTGCCAGCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCata                              >  1:2176495/1‑50 (MQ=255)
                                                           ccGTGGTGCCAGCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCATATAGGCCAGCCAGTCGCTGa           <  1:4110835/69‑1 (MQ=255)
                                                           ccGTGGTGCCAGCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCATATAGGCCAGCCAGTCGCTGAt          <  1:2143317/70‑1 (MQ=255)
                                                             gtggtgCCAGCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCATATAGGCCAGCCAGTCGCTGATCa        <  1:3433595/70‑1 (MQ=255)
                                                              tggtgCCAGCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCATATAGGCCAGCCAGTc                <  1:1478290/61‑1 (MQ=255)
                                                              tggtgCCAGCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCATATAGGCCAGCCAGTCGCTg            <  1:1234303/65‑1 (MQ=255)
                                                              tggtgCCAGCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCATATAGGCCAGCCAGTCGCTGATCATg      <  1:3444243/71‑1 (MQ=255)
                                                               ggtgCCAGCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCATATAGGCCAGCCAGTCGCTGATCATgg     >  1:1208445/1‑71 (MQ=255)
                                                                gtgCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCATATAGGCCAGCCAGTCGCTGATCATGGt    <  1:19157/71‑1 (MQ=255)
                                                                gtgCCAGCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCATATAGGCCAGCCAGTCGCTGATCATGGt    <  1:212842/71‑1 (MQ=255)
                                                                  gCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCATATAGGCCAGCCAGTCGCt             >  1:3597900/1‑60 (MQ=255)
                                                                  gCCAGCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCATATAGGCCAGCCAGTCGCTGATCATGGTGa  >  1:3015578/1‑71 (MQ=255)
                                                                   ccAGCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCata                              <  1:3744402/42‑1 (MQ=255)
                                                                   ccAGCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCata                              <  1:907712/42‑1 (MQ=255)
                                                                   ccAGCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCATATAGGCCAGCCAGTCGCTGATCATGGt    <  1:2757515/68‑1 (MQ=255)
                                                                      |                                                                  
TAGGCCAGCCATATCCAGCAATGCAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCAGCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCATATAGGCCAGCCAGTCGCTGATCATGGTGA  >  minE/967746‑967882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑