| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 974,990 | A→T | 33.9% | intergenic (+742/+250) | lsrB → / ← ydeH | AI2 transporter/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 974,990 | 0 | A | T | 33.9% | 63.0 / 54.6 | 56 | intergenic (+742/+250) | lsrB/ydeH | AI2 transporter/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (16/21); new base T (9/10); total (25/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
ACTGGCAAATTCTGACACCTGCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCTAAGTAAGTTGCCAGGGT > minE/974922‑975057 | aCTGGCAAATTCTGACACCTGCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATACt < 1:1322411/71‑1 (MQ=255) tGGCAAATTCTGACACCTGCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAAttcttc > 1:92376/1‑71 (MQ=255) tGGCAAATTCTGACACCTGCACGACATGCTTCTTCATCATTAGCCGCTATGACAATAATGATAAATACTTc > 1:4133488/1‑71 (MQ=255) gCAAATTCTGACACCTGCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATTCTTCGc < 1:680050/71‑1 (MQ=255) gCAAATTCTGACACCTGCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATACTTCGc > 1:2949272/1‑71 (MQ=255) aaTTCTGACACCTGCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATACTTCGcc > 1:3121587/1‑69 (MQ=255) acacCTGCACGCCATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATACTTCGCCCCCg > 1:1465120/1‑66 (MQ=255) acacCTGCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATACTTCGCCCCCg > 1:1469923/1‑66 (MQ=255) acCTGCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATTCTTCGCCCCCGTAGCGAt < 1:1126570/71‑1 (MQ=255) cTGCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATTCTTCGCCCCCGTAGCGATa > 1:3605117/1‑70 (MQ=255) tGCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATACTTCg < 1:2271201/55‑1 (MQ=255) cACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAAcc > 1:3818603/1‑71 (MQ=255) cACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAAcc < 1:2791526/71‑1 (MQ=255) cACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAAcc > 1:905446/1‑71 (MQ=255) cGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATACTTCGCCCCCGTAGCGa > 1:305697/1‑63 (MQ=255) cGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGt < 1:439845/71‑1 (MQ=255) cGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGt < 1:2717519/71‑1 (MQ=255) gACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGtt > 1:3497132/1‑71 (MQ=255) gACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGtt < 1:2681361/71‑1 (MQ=255) cATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATTCTTCGCCCCCGTAGCGAt < 1:1740417/61‑1 (MQ=255) cATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTc < 1:1795957/71‑1 (MQ=255) tGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATACTTCGCCCCCGTAGCg < 1:3804669/57‑1 (MQ=255) tGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGt < 1:993138/71‑1 (MQ=255) cttcttCATCATTAGCCGCTTTGACAATAATGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTaa > 1:714515/1‑71 (MQ=255) cttcttCATCATTAGCCGCTTTGACAATAATGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTaa > 1:582925/1‑71 (MQ=255) cttcttCATCATTAGCCGCTTTGACAATAATGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTaa > 1:3887948/1‑71 (MQ=255) ttcttcATCATTAGCCGCTTTGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTc < 1:725386/66‑1 (MQ=255) ttcttcATCATTAGCCGCTTTGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTc < 1:2371065/66‑1 (MQ=255) cttcATCATTAGCCGCTTTGACAATAATGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGt < 1:2903284/66‑1 (MQ=255) cttcATCATTAGCCGCTTTGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGTAAt < 1:3862448/69‑1 (MQ=255) cttcATCATTAGCCGCTTTGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGTAATc < 1:2062768/70‑1 (MQ=255) tcatcaTTAGCCGCTTTGACAATAATGATAAATTCt < 1:88530/36‑1 (MQ=255) tcatcaTTAGCCGCATTGACAATAATGATAAATACTTCGc > 1:2767199/1‑40 (MQ=255) catcatTAGCCGCTTTGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCAcgc > 1:62618/1‑71 (MQ=255) atcatTAGCCGCTTTGACAATAATGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCAcgcg > 1:1664071/1‑71 (MQ=255) atcatTAGCCGCTTTGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCAcgcg > 1:3092536/1‑71 (MQ=255) tcatTAGCCGCTTTGACAATAATGATAAATTCTTCGCCCCCGTAGCGATAAACCg < 1:1764931/55‑1 (MQ=255) tcatTAGCCGCTTTGACAATAATGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCAc < 1:728316/67‑1 (MQ=255) tcatTAGCCGCTTTGACAATAATGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCAc < 1:3492683/67‑1 (MQ=255) atTAGCCGCTTTGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTcc < 1:3913614/71‑1 (MQ=255) ttAGCCGCTTTGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTcc > 1:2361656/1‑70 (MQ=255) ttAGCCGCTTTGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCa < 1:2184743/71‑1 (MQ=255) aGCCGCTTTGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGTaa > 1:3890376/1‑58 (MQ=255) gACAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGTAAt < 1:1633997/50‑1 (MQ=255) aataatGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCtaagtaag < 1:1184634/71‑1 (MQ=255) ataatGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCa < 1:3706022/56‑1 (MQ=255) taatGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCt > 1:2084968/1‑62 (MQ=255) aatGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCaa > 1:3947266/1‑55 (MQ=255) aatGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAAc > 1:761296/1‑56 (MQ=255) atGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTaa > 1:3318352/1‑42 (MQ=255) aTAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCTAAGTAAGTTGCCAg < 1:2689768/71‑1 (MQ=255) tAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCTAAGTAAGTTGCCAgg < 1:991727/71‑1 (MQ=255) aaaTTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCAcg > 1:1653425/1‑42 (MQ=255) aaaTACTTCGCCCCCGTAGCGATAAACCGTTTCGTaa > 1:3982209/1‑37 (MQ=255) aaTACTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCTAAGTAAGTTg < 1:1745302/64‑1 (MQ=255) aaTACTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCTAAGTAAGTTGCCAGGGt < 1:1084051/71‑1 (MQ=255) aaTACTTCG‑CCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCTAAGTAAGTTg < 1:1690545/63‑1 (MQ=255) | ACTGGCAAATTCTGACACCTGCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCTAAGTAAGTTGCCAGGGT > minE/974922‑975057 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |