| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 204,556 | A→G | 100% | intergenic (+156/‑28) | alaV → / → rrlH | tRNA‑Ala/23S ribosomal RNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 204,556 | 0 | A | G | 100.0% | 242.4 / NA | 80 | intergenic (+156/‑28) | alaV/rrlH | tRNA‑Ala/23S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (44/36); total (44/36) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AAATTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAG > minE/204492‑204622 | aaaTTGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGc > 1:2297893/1‑70 (MQ=255) ttGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAg > 1:2081005/1‑70 (MQ=255) ttGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa < 1:2399693/71‑1 (MQ=255) gAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGaa < 1:2938891/70‑1 (MQ=255) gAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCg > 1:2840291/1‑71 (MQ=255) atgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtg > 1:807977/3‑71 (MQ=255) tgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt < 1:1674854/70‑1 (MQ=19) gAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACAt > 1:174457/1‑59 (MQ=255) aaaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt < 1:2498090/69‑1 (MQ=25) aaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAg > 1:336678/1‑71 (MQ=21) aaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAg < 1:3867702/71‑1 (MQ=21) aGTTGTTCGTGAGTCTCTCTAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAgg > 1:2869225/1‑71 (MQ=11) aGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGa > 1:3517299/1‑69 (MQ=25) aGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAgg > 1:14312/1‑71 (MQ=21) aGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAgg > 1:3839578/1‑71 (MQ=21) aGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAgg > 1:2782945/1‑71 (MQ=21) aGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAgg > 1:602472/1‑71 (MQ=21) gttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAg > 1:3634428/1‑69 (MQ=25) gttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGt < 1:3584810/71‑1 (MQ=21) gttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGt < 1:979828/71‑1 (MQ=21) ttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGt > 1:1978912/1‑70 (MQ=25) tgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGtt > 1:879585/1‑70 (MQ=25) gttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGtt > 1:1266486/1‑69 (MQ=25) cGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtg < 1:2360279/59‑1 (MQ=255) cGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGc < 1:2382319/70‑1 (MQ=25) gTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCg < 1:719539/53‑1 (MQ=255) tGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGc < 1:2413241/38‑1 (MQ=255) gAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCg < 1:1598572/68‑1 (MQ=25) gAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGAc > 1:2681595/1‑70 (MQ=25) aGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtg > 1:2036457/1‑55 (MQ=255) aGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACt > 1:1637641/1‑70 (MQ=25) gTCTCTCAAATTTTCGCAACACGATGATGGATCTCAAGAAACATCTTCGGGTTGTGAGGTTaa > 1:3473042/1‑63 (MQ=11) tctctcAAATTTTCGCAACACGATGATGGATCGCAAGaa < 1:1819326/39‑1 (MQ=255) tctctcAAATTTTCGCAACACGATGATGGATCGCAAGaa < 1:2163033/39‑1 (MQ=255) tctctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAg > 1:4017795/1‑71 (MQ=21) tctctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAg > 1:4090042/1‑71 (MQ=21) tctcAAATTTTCGCAACACGATGATGGATCGCAAGAAAc < 1:3024167/39‑1 (MQ=255) tctcAAATTTTCGCAACACGATGATGGATCGCAAGAAAc < 1:1176175/39‑1 (MQ=255) tctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACa > 1:1715446/1‑40 (MQ=255) tctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg > 1:3520536/1‑71 (MQ=21) tcAAATTTTCTCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg < 1:1599857/69‑1 (MQ=255) tcAAATTTTCGCAACACGATGATGGATCGCAAGaaa > 1:3271226/1‑36 (MQ=255) tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgtg < 1:4005378/51‑1 (MQ=255) tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgtg < 1:906575/51‑1 (MQ=255) tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAgg > 1:458411/1‑54 (MQ=255) tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAg < 1:527752/53‑1 (MQ=255) tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg < 1:1421099/69‑1 (MQ=25) tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTa > 1:3710637/1‑71 (MQ=21) tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTa > 1:3724147/1‑71 (MQ=21) cAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTa > 1:2618363/1‑70 (MQ=25) cAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTa > 1:2555382/1‑70 (MQ=25) cAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTa > 1:1740195/1‑70 (MQ=25) aaaTTTTCGCACCACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCCTaca > 1:1571338/1‑71 (MQ=11) aaaTTTTCGCAACACGATGATGGATCGCAAGAAACTTCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca > 1:2726173/1‑71 (MQ=255) aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTa > 1:1052432/1‑63 (MQ=25) aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca > 1:1280037/1‑71 (MQ=21) aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca > 1:1947284/1‑71 (MQ=21) aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTa < 1:1889751/69‑1 (MQ=25) aaTTTTCGCAACACGATGATGGATCGCAAGAAACATCttt < 1:3542613/40‑2 (MQ=14) aaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTacac < 1:3548510/71‑1 (MQ=21) aTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGa > 1:3949319/1‑58 (MQ=255) aTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGa > 1:3594495/1‑58 (MQ=255) aTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACg < 1:345356/71‑1 (MQ=21) aTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACg < 1:1920382/71‑1 (MQ=21) ttttCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACgg < 1:3129898/71‑1 (MQ=21) ttCGCAACACGATGATGGATCGCAAGACACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGt < 1:2317397/70‑1 (MQ=255) tCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTg < 1:2624344/70‑1 (MQ=25) tCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTg < 1:3742597/70‑1 (MQ=25) tCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTg < 1:3596700/70‑1 (MQ=25) tCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTg < 1:795422/70‑1 (MQ=25) tCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTg < 1:2792616/70‑1 (MQ=25) cacGATGATGGATCGCAAGAAACATCTTCGGGTtgt < 1:1330722/36‑1 (MQ=255) cacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACg < 1:2740648/61‑1 (MQ=25) cacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGccc < 1:2481025/71‑1 (MQ=21) cacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGccc > 1:4072523/1‑71 (MQ=21) acGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACgg > 1:3903780/1‑61 (MQ=25) acGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCt > 1:1172109/1‑71 (MQ=21) cGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACTGTGGATGCCCTg > 1:1836236/1‑71 (MQ=255) cGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTg > 1:188438/1‑71 (MQ=21) gatgatGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg > 1:1260922/1‑52 (MQ=255) tgatggATCGCAAGAAACATCTTCGGGTTGTGAGTTTAAGCGACTaa < 1:3947170/47‑1 (MQ=11) tgatggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGc < 1:819078/70‑1 (MQ=25) tgatggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCa < 1:2917344/71‑1 (MQ=21) tgatggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCa < 1:1063428/71‑1 (MQ=21) gatggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTgg > 1:762040/1‑59 (MQ=255) gatggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAg > 1:2925651/1‑71 (MQ=21) atggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTacac < 1:1297117/53‑1 (MQ=255) atggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTacac < 1:1996176/53‑1 (MQ=255) atggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAg < 1:410784/70‑1 (MQ=25) | AAATTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAG > minE/204492‑204622 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |