| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 980,278 | C→T | 9.5% | V9V (GTG→GTA) | cspI ← | cold shock protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 980,278 | 0 | C | T | 9.5% | 260.4 / 12.8 | 84 | V9V (GTG→GTA) | cspI | cold shock protein |
| Reads supporting (aligned to +/- strand): ref base C (39/37); new base T (6/2); total (45/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTC > minE/980209‑980347 | gACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCa > 1:3146072/1‑71 (MQ=255) aaaCACATCTTTACTACCATCAACAGGAGAAATAAAGCCGAAACCTTTATCAGCGTTAAACCATTTTACt > 1:304054/1‑70 (MQ=255) acacATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTaaa < 1:3288749/71‑1 (MQ=255) cacaTCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAAc > 1:3043582/1‑71 (MQ=255) caTCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAAc > 1:3386561/1‑69 (MQ=255) gtCTTTGCTGCCATCATCAGGAGTGATAAAGCCAAAACCTTTATCTGCGTTAAACCATTTTACTAAACCAg > 1:2390159/2‑71 (MQ=9) tttGCTGCCATCATCAGGAGTGATAAAGCCAAAACCTTTATCTGCGTTAAACCATTTTACTAAACCAGTCa > 1:1965061/1‑71 (MQ=12) tgctgcCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCa > 1:43453/1‑57 (MQ=255) tgctgcCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGt < 1:3571953/67‑1 (MQ=255) tgctgcCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCAt > 1:566309/1‑70 (MQ=255) ccATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt < 1:1421616/70‑1 (MQ=255) tCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTc < 1:2476967/48‑1 (MQ=255) tCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAAc > 1:3936028/1‑55 (MQ=255) tttCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCAc > 1:3815115/1‑48 (MQ=255) tttCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCAt < 1:3776268/60‑1 (MQ=255) tttCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTa > 1:2373808/1‑67 (MQ=255) tCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGa < 1:282312/67‑1 (MQ=255) tCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACAt < 1:1702742/70‑1 (MQ=255) tCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACAt < 1:1899011/70‑1 (MQ=255) tCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACAt < 1:1131223/70‑1 (MQ=255) cGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGa < 1:2707690/66‑1 (MQ=255) ggCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAat < 1:3668892/71‑1 (MQ=255) gagTGATAAAGCCAAAACCTTTATCTGCGTTAAACCATTTTACTAAACCAGTCATTTTATTAGACATAttt < 1:3732265/69‑1 (MQ=255) gCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAat > 1:1403659/1‑70 (MQ=255) gCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAat < 1:2375025/70‑1 (MQ=255) tgatgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt < 1:1443865/57‑1 (MQ=255) gatgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAg < 1:141036/59‑1 (MQ=255) atgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAACCCAGTCATTTTGTTAGACATAATTATTAc > 1:1118278/1‑71 (MQ=255) atgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTc > 1:2430700/1‑48 (MQ=255) atgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTAc > 1:1791914/1‑71 (MQ=255) atgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTAc > 1:2435792/1‑71 (MQ=255) atgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTAc > 1:3689580/1‑71 (MQ=255) atgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTAc > 1:681991/1‑71 (MQ=255) aaaCCAAAACCTTTTTCAGGGTTAAACCATTTCACTaaa < 1:2146255/39‑1 (MQ=255) aaaCCAAAACCTTTTTCAGGGTTAAACCATTTCACTa < 1:1102343/37‑1 (MQ=255) aaaCCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt > 1:3279076/1‑52 (MQ=255) aaaCCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCtt < 1:3966583/71‑1 (MQ=255) aaCCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGAc > 1:3125964/1‑56 (MQ=255) aaCCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCttt < 1:2234344/71‑1 (MQ=255) aCCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGAc > 1:1521040/1‑55 (MQ=255) aCCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGAc > 1:3450140/1‑55 (MQ=255) aCCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGAGATAATTATTACCtttt > 1:3562023/1‑71 (MQ=255) aCCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAatt < 1:275604/61‑1 (MQ=255) aCCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCtttt < 1:2858166/71‑1 (MQ=255) aCCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCtttt < 1:1097479/71‑1 (MQ=255) aCCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCtttt < 1:2667077/71‑1 (MQ=255) ccAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCAtttt < 1:3474137/47‑1 (MQ=255) ccAAAACCTTTTTCAGGGTTAAACCATTTCACTAAAACAGTCAtttt > 1:1181557/1‑47 (MQ=255) aaaCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTg < 1:1747438/45‑1 (MQ=255) aaCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTgaaga < 1:1520341/71‑1 (MQ=255) aaCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTgaaga < 1:2374128/71‑1 (MQ=255) aCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAatta > 1:3751185/1‑56 (MQ=255) aCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTgaagaa < 1:224202/71‑1 (MQ=255) aCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTgaaga > 1:2403280/1‑70 (MQ=255) aCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTgaag > 1:2613212/1‑69 (MQ=255) ccTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAatta > 1:1797803/1‑55 (MQ=255) cTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCAt < 1:59532/37‑1 (MQ=255) cTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAAt < 1:3350055/71‑1 (MQ=255) tttttCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt < 1:3269716/41‑1 (MQ=255) tttttCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTgaag < 1:681908/66‑1 (MQ=255) tttatCAGCGTTAAACCATTTTACTAAACCAGTCATTTTATTTGa < 1:4067809/45‑1 (MQ=255) ttttCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAAt > 1:1281556/1‑69 (MQ=255) tttCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAAtt < 1:3362705/69‑1 (MQ=255) tttCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAg > 1:909849/1‑71 (MQ=255) tatCAGCGTTAAACCATTTTACTAAACCAGTCATTTTATTTGACa > 1:2393450/3‑45 (MQ=255) tatCAGCGTTAAACCATTTTACTAAACCAGTCATTTTATTTGACa > 1:2297928/3‑45 (MQ=255) tatCAGCGTTAAACCATTTTACTAAACCAGTCATTTTATTTGACa > 1:3999664/3‑45 (MQ=255) aGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACAt > 1:3624282/1‑42 (MQ=255) aGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACAt > 1:3894000/1‑42 (MQ=255) aGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACAt > 1:4118427/1‑42 (MQ=255) ggTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAat < 1:1486013/43‑1 (MQ=255) ggTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGaaa > 1:1631578/1‑61 (MQ=255) ttAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTga < 1:2276828/54‑1 (MQ=255) ttAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTg < 1:1074534/53‑1 (MQ=255) tAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGaaa < 1:2924969/58‑1 (MQ=255) aaaCCATTTCACTAAACCAGTCATTTTGTTAGACATaa > 1:1884872/1‑38 (MQ=255) aaaCCATTTCACTAAACCAGTCATTTTGTTAGACATAatta > 1:1082944/1‑41 (MQ=255) aaaCCATTTCACTAAACCAGTCATTTTGTTAGACATAatta > 1:313392/1‑41 (MQ=255) aaaCCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGcc < 1:3582828/63‑1 (MQ=255) aaaCCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTggg > 1:1012658/1‑69 (MQ=255) aaaCCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTggg > 1:3031080/1‑69 (MQ=255) aCCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCttt > 1:1177447/1‑47 (MQ=255) ccATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGaa < 1:313743/71‑1 (MQ=255) tCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTc > 1:1406659/1‑71 (MQ=255) tCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATCGTc > 1:3376049/1‑71 (MQ=255) cACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTg < 1:3570927/42‑1 (MQ=255) | GACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTC > minE/980209‑980347 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |