| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,014,491 | A→C | 8.3% | L526L (CTT→CTG) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,014,491 | 0 | A | C | 8.3% | 117.6 / 7.0 | 48 | L526L (CTT→CTG) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base A (26/18); new base C (2/2); total (28/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
TATTTCACACAGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCATTCCCAGTTCCGGATATTC > minE/1014423‑1014559 | tATTTCACACAGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTGATTTCCTTTATCATCCACAAg > 1:3916214/1‑71 (MQ=255) cacacaGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATa < 1:3768868/70‑1 (MQ=255) cacacaGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATa > 1:2675405/1‑70 (MQ=255) cacacaGCGGGTGCATTGTGTGAGTTGTATCTGCGGGAAGAAGTCATTTCCTTTATCATCCACAAGGATa > 1:2196494/1‑70 (MQ=255) ggTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGa < 1:3088009/71‑1 (MQ=255) aTTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCt < 1:3400431/71‑1 (MQ=255) gtgtgAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATaaa > 1:2575239/1‑55 (MQ=255) gtgtgAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTc > 1:1184631/1‑70 (MQ=255) gtgtgAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTc < 1:3123489/70‑1 (MQ=255) gtgtgAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTc < 1:2966079/70‑1 (MQ=255) tgAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACt > 1:3934044/1‑71 (MQ=255) gAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTc < 1:3391403/66‑1 (MQ=255) aGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAgg > 1:1715216/1‑45 (MQ=255) aGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAgg > 1:682881/1‑45 (MQ=255) aGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACtt > 1:1027424/1‑70 (MQ=255) gTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATc > 1:1944126/1‑58 (MQ=255) tATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAAttt < 1:3223003/71‑1 (MQ=255) tttgctgGAAGAAGTCGTTACCTTTGTCATCGACCAGGATa < 1:1577184/39‑1 (MQ=12) tgctgGAAGAAGTCATTTCCTTTATCATCCACAAGGATaaa > 1:452601/1‑41 (MQ=255) tgctgGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGcc > 1:3910604/1‑45 (MQ=255) tgctgGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCtt > 1:2706195/1‑55 (MQ=255) tgctgGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTc > 1:3613336/1‑56 (MQ=255) gctgGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTc > 1:1898592/1‑68 (MQ=255) ctgGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAg < 1:4141530/70‑1 (MQ=255) tgGAAGAAGTCATTTCCTTTATCATCCACAAGGATa < 1:2365567/36‑1 (MQ=255) tgGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGAt > 1:3589381/1‑71 (MQ=255) gaagaaGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGAtag < 1:1562773/71‑3 (MQ=255) aagaagTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGc > 1:656430/1‑71 (MQ=255) agaagTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCt > 1:405931/1‑71 (MQ=255) agTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAAtttt > 1:1865458/1‑58 (MQ=255) agTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTcc < 1:1477410/71‑1 (MQ=255) gTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGaa < 1:1355953/39‑1 (MQ=255) gTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGaa < 1:2360544/39‑1 (MQ=255) gTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGc < 1:1592535/66‑1 (MQ=255) gTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGc < 1:3833063/66‑1 (MQ=255) gTCATTTCCTTTATCATCAACAAGGATAAACGCCGGGaa < 1:3602313/39‑1 (MQ=255) aTTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTcc > 1:2413833/1‑67 (MQ=255) aTTTCCTTTATCATACACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTcc > 1:841826/1‑67 (MQ=255) ttCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCATTcc > 1:96595/1‑70 (MQ=255) taccTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCa > 1:1228243/3‑42 (MQ=255) accTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATAcc > 1:3707192/2‑69 (MQ=255) cTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCATTCCCAg < 1:1029662/70‑1 (MQ=255) tATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCATTCCAAGTTcc > 1:1268592/1‑71 (MQ=255) atcatcCACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCATTCCCAg > 1:2757852/1‑66 (MQ=255) tcatcCACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCATTCCCAGTTCCg < 1:301487/70‑1 (MQ=255) atcCACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCATTCCCAGTTCCgg > 1:1075586/1‑69 (MQ=255) atcCACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCATTCCCAGTTCCGGa < 1:757433/70‑1 (MQ=255) tcCACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGc > 1:3476269/1‑50 (MQ=255) cacaAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCtt > 1:913505/1‑50 (MQ=255) acaAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCATTCCCAGTTCCGGATATTc < 1:3912123/71‑1 (MQ=255) caAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGa > 1:3359455/1‑42 (MQ=255) caAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGa > 1:1392605/1‑42 (MQ=255) aaGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCa < 1:2514489/39‑1 (MQ=255) | TATTTCACACAGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCATTCCCAGTTCCGGATATTC > minE/1014423‑1014559 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |