| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,014,644 | T→A | 13.3% | K475N (AAA→AAT) ‡ | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,014,644 | 0 | T | A | 13.3% | 74.4 / 5.3 | 30 | K475N (AAA→AAT) ‡ | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base T (17/9); new base A (2/2); total (19/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01 | |||||||||||
AATACTTCCCTGCGCCAATACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGC > minE/1014578‑1014712 | aaTACTTCCCTGCGCCAATACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTa > 1:2172421/1‑70 (MQ=255)aaTACTTCCCTGCGCCAATACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTa > 1:681869/1‑70 (MQ=255) aTACTTCCCTGCGCCAATACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGtttttt < 1:2755555/68‑1 (MQ=255) aTACTTCCCTGCGCCAATACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTAc < 1:1030068/70‑1 (MQ=255) tACTTCCCTGCGCCAATACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAg > 1:910951/1‑71 (MQ=255) cTTCCCTGCGCCAATACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGtttt > 1:2968314/1‑63 (MQ=255) ccTGCGCCAATACAGCGGCCGGCCCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATcc > 1:343947/1‑71 (MQ=255) tGCGCCAATACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGtttt > 1:1129935/1‑57 (MQ=255) gcgcCAATACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAg > 1:621400/1‑62 (MQ=255) gtACCGCCGCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACctgc > 1:2303198/2‑71 (MQ=255) tACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTc < 1:1881849/64‑1 (MQ=255) aCAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACctgctg > 1:4007781/1‑71 (MQ=255) aCAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACctgctg > 1:2401664/1‑71 (MQ=255) gCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTgctggct > 1:1782672/1‑71 (MQ=255) cGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTTCAGGCATCCGTCACCTgctggct > 1:1105988/1‑70 (MQ=255) ggCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGc > 1:3350080/1‑71 (MQ=255) ccGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATc > 1:3629039/1‑52 (MQ=255) cGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGtttttt < 1:2468482/42‑1 (MQ=255) ggACCACCGATACTGCCAAGGTAGAAGCCGCCGTGttttt < 1:3900850/40‑1 (MQ=255) gACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTAc > 1:2564995/1‑42 (MQ=255) accaccGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTa > 1:4133039/1‑40 (MQ=255) ccaccGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGc > 1:4037026/1‑44 (MQ=255) ccGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTc > 1:446543/1‑44 (MQ=12) cGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGt < 1:708229/46‑1 (MQ=255) aTACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGc < 1:1980606/71‑1 (MQ=255) cTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACctgc > 1:2080275/1‑48 (MQ=255) ccgaGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCt < 1:2282755/57‑1 (MQ=255) aaGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGa > 1:586954/1‑71 (MQ=255) aGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGTCTGCGGTTGCCTTTCGCCAGCATGAt < 1:2841483/71‑1 (MQ=255) aGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGc < 1:3700317/66‑1 (MQ=255) aGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGc < 1:2502461/66‑1 (MQ=255) ggTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACctgct < 1:2800780/42‑1 (MQ=255) gAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATa < 1:562015/70‑1 (MQ=255) gccgTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGc > 1:3228494/1‑71 (MQ=255) gtgtTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGAt < 1:227388/57‑1 (MQ=255) ttttttACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGa < 1:1671168/53‑1 (MQ=255) ttttttACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTgcg > 1:4009513/1‑71 (MQ=255) ttttttACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTgc < 1:1063912/70‑1 (MQ=255) ttttACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATc > 1:2770714/1‑53 (MQ=255) ttttACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTgcgc < 1:4126777/70‑1 (MQ=255) | AATACTTCCCTGCGCCAATACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGC > minE/1014578‑1014712 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |