| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,014,818 | A→G | 6.9% | H417H (CAT→CAC) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,014,818 | 0 | A | G | 6.9% | 164.8 / 3.8 | 58 | H417H (CAT→CAC) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base A (27/27); new base G (2/2); total (29/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01 | |||||||||||
GTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATAT > minE/1014753‑1014888 | gTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATc > 1:1537813/1‑71 (MQ=255) ttGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCt < 1:2487878/71‑1 (MQ=255) tGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCtt > 1:2524295/1‑71 (MQ=255) gggCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCtt < 1:4091952/70‑1 (MQ=255) ggCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTTATCttt < 1:8670/70‑1 (MQ=255) ggCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCttt < 1:498954/70‑1 (MQ=255) gCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGa < 1:1929100/71‑1 (MQ=255) cAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATg < 1:1344157/71‑1 (MQ=255) gagaACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGga > 1:944244/1‑56 (MQ=255) gaACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTg > 1:833732/1‑71 (MQ=255) aaCCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCttt > 1:551054/1‑61 (MQ=255) aaCCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCttt > 1:4093745/1‑61 (MQ=255) aaCCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGa > 1:2627726/1‑63 (MQ=255) gCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGa < 1:1054043/55‑1 (MQ=255) gCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGa < 1:270241/55‑1 (MQ=255) aTAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGa > 1:2907868/1‑53 (MQ=255) tAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCt < 1:2888106/48‑1 (MQ=255) tAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCtt > 1:423521/1‑70 (MQ=255) aaCCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCa > 1:3085683/1‑71 (MQ=255) aCCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCt < 1:504573/46‑1 (MQ=255) aCCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCAc > 1:2407409/1‑71 (MQ=255) aCCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCAc > 1:916124/1‑71 (MQ=255) cTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCttt > 1:3807066/1‑46 (MQ=255) ttCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCt < 1:2291594/43‑1 (MQ=255) ttCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCt < 1:2107492/43‑1 (MQ=255) ttCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATg < 1:1870067/49‑1 (MQ=255) ccGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTa > 1:1097324/1‑71 (MQ=255) cGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTat < 1:3468916/71‑1 (MQ=255) cGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTa < 1:556199/70‑1 (MQ=255) gCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCtt < 1:2954730/39‑1 (MQ=255) ttGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGa > 1:2898046/1‑37 (MQ=255) ttGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGa > 1:1232856/1‑37 (MQ=255) ttGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCg > 1:2590988/1‑71 (MQ=255) ttGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCg > 1:4077071/1‑71 (MQ=255) ttGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCg > 1:2827994/1‑71 (MQ=255) ttGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCg > 1:3619533/1‑71 (MQ=255) tGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGa > 1:2293454/1‑36 (MQ=255) tGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGa > 1:3995564/1‑36 (MQ=255) tGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGt < 1:1855589/60‑1 (MQ=255) tGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGt < 1:3240787/60‑1 (MQ=255) cGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCtt > 1:607638/1‑71 (MQ=255) cGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCtt > 1:2561392/1‑71 (MQ=255) ggACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCttt < 1:1227072/71‑1 (MQ=255) aCCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGt < 1:3873275/53‑1 (MQ=255) gcgTAGTAGATCGGGTGATCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGctt > 1:189733/1‑71 (MQ=255) agtaAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCttt < 1:2832601/61‑1 (MQ=255) taAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTatcc < 1:4058249/48‑1 (MQ=255) gaTCGGGTGATCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTgtg > 1:4086970/2‑69 (MQ=255) aaTCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGtt < 1:1008676/62‑1 (MQ=255) aaTCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAg > 1:1604695/1‑71 (MQ=255) aaTCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAg > 1:609762/1‑71 (MQ=255) aaTCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAg > 1:3266590/1‑71 (MQ=255) aTCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGc < 1:518570/71‑1 (MQ=255) tCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGa < 1:2043162/68‑1 (MQ=255) ggATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCtt < 1:1294073/52‑1 (MQ=255) ggATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGttt < 1:760663/59‑1 (MQ=255) ggATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCaa > 1:3321036/1‑70 (MQ=255) ggATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAat < 1:137192/71‑1 (MQ=255) ggATGATCTTTGATGTACTGCGGCAGCCCTTCACAGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAat < 1:2608323/71‑1 (MQ=255) gatgatCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCtt < 1:992132/51‑1 (MQ=255) gatgatCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAata < 1:52035/71‑1 (MQ=255) atgatCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAatat < 1:3800675/71‑1 (MQ=255) | GTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATAT > minE/1014753‑1014888 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |