| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,014,839 | C→A | 8.1% | L410L (CTG→CTT) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,014,839 | 0 | C | A | 8.1% | 203.2 / 3.0 | 61 | L410L (CTG→CTT) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base C (35/21); new base A (3/2); total (38/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01 | |||||||||||
TAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTG > minE/1014777‑1014908 | tAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCtt > 1:423521/1‑70 (MQ=255) aaCCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCa > 1:3085683/1‑71 (MQ=255) aCCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCAc > 1:2407409/1‑71 (MQ=255) aCCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCAc > 1:916124/1‑71 (MQ=255) ccGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTa > 1:1097324/1‑71 (MQ=255) cGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTat < 1:3468916/71‑1 (MQ=255) cGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTa < 1:556199/70‑1 (MQ=255) ttGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCg > 1:2827994/1‑71 (MQ=255) ttGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCg > 1:3619533/1‑71 (MQ=255) ttGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCg > 1:2590988/1‑71 (MQ=255) ttGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCg > 1:4077071/1‑71 (MQ=255) tGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGt < 1:3240787/60‑1 (MQ=255) tGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGt < 1:1855589/60‑1 (MQ=255) cGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCtt > 1:2561392/1‑71 (MQ=255) cGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCtt > 1:607638/1‑71 (MQ=255) ggACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCttt < 1:1227072/71‑1 (MQ=255) aCCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGt < 1:3873275/53‑1 (MQ=255) gcgTAGTAGATCGGGTGATCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGctt > 1:189733/1‑71 (MQ=255) agtaAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCttt < 1:2832601/61‑1 (MQ=255) taAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTatcc < 1:4058249/48‑1 (MQ=255) gaTCGGGTGATCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTgtg > 1:4086970/2‑69 (MQ=255) aaTCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGtt < 1:1008676/62‑1 (MQ=255) aaTCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAg > 1:1604695/1‑71 (MQ=255) aaTCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAg > 1:609762/1‑71 (MQ=255) aaTCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAg > 1:3266590/1‑71 (MQ=255) aTCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGc < 1:518570/71‑1 (MQ=255) tCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGa < 1:2043162/68‑1 (MQ=255) ggATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCtt < 1:1294073/52‑1 (MQ=255) ggATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGttt < 1:760663/59‑1 (MQ=255) ggATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCaa > 1:3321036/1‑70 (MQ=255) ggATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAat < 1:137192/71‑1 (MQ=255) ggATGATCTTTGATGTACTGCGGCAGCCCTTCACAGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAat < 1:2608323/71‑1 (MQ=255) gatgatCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCtt < 1:992132/51‑1 (MQ=255) gatgatCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAata < 1:52035/71‑1 (MQ=255) atgatCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAatat < 1:3800675/71‑1 (MQ=255) tgatCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAatat > 1:1479049/1‑70 (MQ=255) tgatCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTg < 1:2796480/57‑1 (MQ=12) gatCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCa > 1:1522929/1‑71 (MQ=255) atCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATctc > 1:749274/1‑69 (MQ=255) tCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAc > 1:3302546/1‑70 (MQ=255) tCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAc > 1:2833578/1‑70 (MQ=255) tCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAc > 1:2289481/1‑70 (MQ=255) cTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGct > 1:3325229/1‑42 (MQ=255) cTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGct > 1:14033/1‑42 (MQ=255) cTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGct > 1:893859/1‑42 (MQ=255) cTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAata > 1:1216289/1‑65 (MQ=255) tttGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAatat < 1:2399079/65‑1 (MQ=255) tGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCg > 1:1927636/1‑53 (MQ=255) tGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCg > 1:338784/1‑53 (MQ=255) tGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAata > 1:2118326/1‑62 (MQ=255) tGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAata > 1:3514414/1‑62 (MQ=255) tGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAcgacc > 1:2708750/1‑70 (MQ=255) gATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAatat < 1:2979892/62‑1 (MQ=255) tACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGttt > 1:175870/1‑44 (MQ=255) tACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACcgacga > 1:4056897/1‑70 (MQ=255) cTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATaa < 1:3068872/71‑1 (MQ=255) cTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATa > 1:145934/1‑70 (MQ=255) gCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGa > 1:2561393/1‑47 (MQ=255) gCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAcgaccga > 1:1849733/1‑63 (MQ=255) ggCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGt < 1:3800950/71‑1 (MQ=255) gCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTg > 1:3339157/1‑71 (MQ=255) | TAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTG > minE/1014777‑1014908 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |