| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,014,953 | T→G | 7.8% | A372A (GCA→GCC) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,014,953 | 0 | T | G | 7.8% | 158.9 / 9.7 | 64 | A372A (GCA→GCC) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base T (34/25); new base G (3/2); total (37/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
CAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCG > minE/1014883‑1015018 | cAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACtgt < 1:3369983/71‑1 (MQ=255) tatCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGc > 1:2048384/1‑70 (MQ=255) tatCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCg < 1:3427878/71‑1 (MQ=255) tCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGa < 1:3551243/70‑1 (MQ=255) tCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAg > 1:3102014/1‑71 (MQ=255) tCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAg > 1:1233175/1‑71 (MQ=255) tCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAg > 1:307299/1‑71 (MQ=255) tCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAg > 1:3304851/1‑71 (MQ=255) tCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAg > 1:144169/1‑71 (MQ=255) aCGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGa < 1:105015/71‑1 (MQ=255) gaccgacGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGAtc < 1:1713631/71‑1 (MQ=255) accgacGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGAtct < 1:4000449/71‑1 (MQ=255) gacgaTAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCtt < 1:1596212/71‑1 (MQ=255) gacgaTAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCtt > 1:2447373/1‑71 (MQ=255) gaTAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCa > 1:2998540/1‑71 (MQ=255) tAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCg < 1:2524493/56‑1 (MQ=255) aaTCGTGCCGTTAAGCGATAAGCGTGTAGAAAAGGGATACTGCGACAACTGTGCGAGGATCTCTTTc > 1:3635067/1‑67 (MQ=255) atggTGCCGGTGAGCGACAAACGAGTGGATACCGGGTATTGCGAAAGCTGGGCGAGGATCTCTTTCATCg < 1:3178752/67‑1 (MQ=255) gTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACg > 1:3123220/1‑71 (MQ=255) gCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATc > 1:3545611/1‑64 (MQ=255) ccGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGtt < 1:1281701/71‑1 (MQ=255) ccGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGt < 1:83058/70‑1 (MQ=255) ccGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGt < 1:2504190/70‑1 (MQ=255) cGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTa < 1:610351/71‑1 (MQ=255) gtgaGCGACAAACGAGTGGATACCGGGTATTGCGAAAGCTGGGCGAGGATCTCTTTCATCGGGCGGTTAAg < 1:2741368/68‑1 (MQ=255) tAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAgg > 1:3111127/1‑71 (MQ=255) aaGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGt > 1:603516/1‑71 (MQ=255) aaGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGAAGGTTAAgg > 1:2035429/1‑70 (MQ=255) aGCGACAAACGAGTGGATACCGGGTATTGCGAAAGCTGGGCGAGGATCTCTTTc > 1:3654381/1‑54 (MQ=255) gCGATAAGCGTGAAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCa < 1:3038885/71‑1 (MQ=255) gATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAg > 1:3914917/1‑65 (MQ=255) gATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAg > 1:1494838/1‑65 (MQ=255) gATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAg > 1:1300614/1‑65 (MQ=255) aTAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGtt < 1:2684268/61‑1 (MQ=255) tAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAAcgc < 1:161114/71‑1 (MQ=255) aaGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAAcgcg > 1:219754/1‑71 (MQ=255) aGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACgcgc < 1:2803762/71‑1 (MQ=255) gtgtAGAAACGGGATACTGCGACAACTGTGCGAGGATctct < 1:2739327/41‑1 (MQ=255) gtgtAGAAACGGGATACTGCGACAACTGTGCGAGGATctct < 1:1189401/41‑1 (MQ=255) gtgtAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCAcc > 1:780386/1‑71 (MQ=255) tAGAAACGGGATACTGCGACAACTGTGCGAGGAtc < 1:1286507/35‑1 (MQ=255) tAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTc < 1:2778625/41‑1 (MQ=255) tAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCt > 1:3112831/1‑71 (MQ=255) gAAACGGGATACTGCGACAACTGTGCGAGGAtctc > 1:3384699/1‑35 (MQ=255) gAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTaa > 1:3276116/1‑52 (MQ=255) gAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTa < 1:1870742/51‑1 (MQ=255) aaCGGGATACTGCGACAACTGTGCGAGGATCTCttt < 1:2843280/36‑1 (MQ=255) cGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCt < 1:2180474/71‑1 (MQ=255) gggATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCaa > 1:3678980/1‑53 (MQ=255) gATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTtct < 1:2101188/71‑1 (MQ=255) cTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAg > 1:3759899/1‑42 (MQ=255) cTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAg > 1:1618018/1‑42 (MQ=255) cTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTc < 1:548376/45‑1 (MQ=255) cTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAAc > 1:3275765/1‑48 (MQ=255) cTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAAc > 1:188160/1‑48 (MQ=255) cTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCg > 1:1738502/1‑56 (MQ=255) cTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCg > 1:1534982/1‑71 (MQ=255) cTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCg > 1:1089664/1‑71 (MQ=255) tGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGc > 1:1858761/1‑71 (MQ=255) gCGAAAGCTGGGCGAGGATCTCTTTCATCGGGCGGTTAAGGTCAAc > 1:1511350/1‑46 (MQ=37) gCGAAAGCTGGGCGAGGATCTCTTTCATCGGGCGGTTAAGGTCAACTTTCACCGCTTCGCCTTCACCGGcc > 1:3034778/1‑70 (MQ=255) cGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGc < 1:1757436/69‑1 (MQ=255) gACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCttt > 1:704309/1‑71 (MQ=255) aaCTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTgcg > 1:591585/1‑71 (MQ=255) cTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAAGGCGCACCGCTTCGCCTTCTCCCGCTTTgcg > 1:2612827/1‑69 (MQ=255) tgtgCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCtt > 1:193981/1‑64 (MQ=255) | CAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCG > minE/1014883‑1015018 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |