| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,014,974 | A→G | 13.1% | R365R (CGT→CGC) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,014,974 | 0 | A | G | 13.1% | 157.0 / 15.4 | 61 | R365R (CGT→CGC) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base A (33/20); new base G (5/3); total (38/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.29e-01 | |||||||||||
GTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTG > minE/1014906‑1015044 | gTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACg > 1:3123220/1‑71 (MQ=255) ccGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGtt < 1:1281701/71‑1 (MQ=255) ccGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGt < 1:83058/70‑1 (MQ=255) ccGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGt < 1:2504190/70‑1 (MQ=255) cGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTa < 1:610351/71‑1 (MQ=255) gtgaGCGACAAACGAGTGGATACCGGGTATTGCGAAAGCTGGGCGAGGATCTCTTTCATCGGGCGGTTAAg < 1:2741368/68‑1 (MQ=255) tAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAgg > 1:3111127/1‑71 (MQ=255) aaGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGt > 1:603516/1‑71 (MQ=255) aaGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGAAGGTTAAgg > 1:2035429/1‑70 (MQ=255) gCGATAAGCGTGAAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCa < 1:3038885/71‑1 (MQ=255) gATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAg > 1:1494838/1‑65 (MQ=255) gATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAg > 1:1300614/1‑65 (MQ=255) gATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAg > 1:3914917/1‑65 (MQ=255) aTAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGtt < 1:2684268/61‑1 (MQ=255) tAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAAcgc < 1:161114/71‑1 (MQ=255) aaGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAAcgcg > 1:219754/1‑71 (MQ=255) aGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACgcgc < 1:2803762/71‑1 (MQ=255) gtgtAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCAcc > 1:780386/1‑71 (MQ=255) tAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCt > 1:3112831/1‑71 (MQ=255) gAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTaa > 1:3276116/1‑52 (MQ=255) gAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTa < 1:1870742/51‑1 (MQ=255) cGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCt < 1:2180474/71‑1 (MQ=255) gggATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCaa > 1:3678980/1‑53 (MQ=255) gATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTtct < 1:2101188/71‑1 (MQ=255) cTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAg > 1:3759899/1‑42 (MQ=255) cTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAg > 1:1618018/1‑42 (MQ=255) cTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTc < 1:548376/45‑1 (MQ=255) cTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAAc > 1:188160/1‑48 (MQ=255) cTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAAc > 1:3275765/1‑48 (MQ=255) cTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCg > 1:1738502/1‑56 (MQ=255) cTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCg > 1:1089664/1‑71 (MQ=255) cTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCg > 1:1534982/1‑71 (MQ=255) tGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGc > 1:1858761/1‑71 (MQ=255) gCGAAAGCTGGGCGAGGATCTCTTTCATCGGGCGGTTAAGGTCAAc > 1:1511350/1‑46 (MQ=37) gCGAAAGCTGGGCGAGGATCTCTTTCATCGGGCGGTTAAGGTCAACTTTCACCGCTTCGCCTTCACCGGcc > 1:3034778/1‑70 (MQ=255) cGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGc < 1:1757436/69‑1 (MQ=255) gACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCttt > 1:704309/1‑71 (MQ=255) aaCTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTgcg > 1:591585/1‑71 (MQ=255) cTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAAGGCGCACCGCTTCGCCTTCTCCCGCTTTgcg > 1:2612827/1‑69 (MQ=255) tgtgCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCtt > 1:193981/1‑64 (MQ=255) gAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTgcgc < 1:3066474/64‑1 (MQ=255) gATCTCTTTCATCGGACGGTTAAGGTGAACGCGCACCGCTTCGCCt < 1:2470173/46‑1 (MQ=255) aTCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTccc < 1:1930734/51‑1 (MQ=255) tctcTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCt > 1:3132604/1‑44 (MQ=255) ctctTTCATCGGGCGGTTAAGGTCAACTTTCACCGCTTCGCCTTCACCGGCCTGGCGCAg < 1:2703865/60‑1 (MQ=255) ctctTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTc > 1:818005/1‑66 (MQ=255) tctTTCATCGGGCGGTTAAGGTCAACTTTCACCGCTTCGCCTTCACCGGCCTGGCGCAg < 1:1604314/59‑1 (MQ=255) ctTTCATCGGGCGGTTAAGGTCAACTTTCACCGCTTCGCCTTCACCGGCCTGGCGCAgtt > 1:1701736/1‑58 (MQ=255) ctTTCATCGGGCGGTTAAGGTCAACTTTCACCGCTTCGCCTTCACCGGCCTGGCGCAgtt > 1:4069418/1‑58 (MQ=255) ctTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTcc > 1:546026/1‑46 (MQ=255) ctTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGat > 1:2833588/1‑70 (MQ=255) tttCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGctc > 1:3367253/1‑60 (MQ=255) tttCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCgg < 1:2316345/66‑1 (MQ=255) tCATCGGGCGGTTAAGGTCAACTTTCACCGCTTCGCCTTCACCGGCCTGGCGCAgtt > 1:1688759/1‑55 (MQ=255) tCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGatata < 1:2174847/70‑1 (MQ=255) tCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGata < 1:4284/68‑1 (MQ=255) cATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATAtt > 1:1361354/1‑71 (MQ=255) aTCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATAttt < 1:4089249/71‑1 (MQ=255) cGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGc > 1:1349687/1‑71 (MQ=255) ggACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGcc > 1:3936515/1‑71 (MQ=255) gACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCt > 1:328254/1‑71 (MQ=255) aCGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTg > 1:612937/1‑71 (MQ=255) | GTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTG > minE/1014906‑1015044 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |