| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,015,076 | C→T | 9.8% | Q331Q (CAG→CAA) ‡ | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,015,076 | 0 | C | T | 9.8% | 212.9 / 11.0 | 71 | Q331Q (CAG→CAA) ‡ | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base C (28/36); new base T (2/5); total (30/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.35e-01 | |||||||||||
CTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAG > minE/1015006‑1015142 | ctcCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATcccc > 1:2102682/1‑71 (MQ=255) ccGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTg < 1:1254966/70‑1 (MQ=255) cGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGAc > 1:1971624/1‑71 (MQ=255) cGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGAc > 1:1914875/1‑71 (MQ=255) cTTTGCGCAGCTCTTCCGGGATATATTTGCCTTGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACgg < 1:4101072/71‑1 (MQ=255) cTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACgg < 1:2770773/71‑1 (MQ=255) cTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACgg < 1:3294437/71‑1 (MQ=255) cTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACgg < 1:1286753/71‑1 (MQ=255) tttGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACgg < 1:2962426/70‑1 (MQ=255) ttGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGtt > 1:763344/1‑71 (MQ=255) aGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCtt > 1:3143774/1‑71 (MQ=255) ctTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCt > 1:3407522/1‑71 (MQ=255) ccGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCttt > 1:2997828/1‑70 (MQ=255) ccGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCttt < 1:550704/70‑1 (MQ=255) gATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGatat < 1:1667321/71‑1 (MQ=255) atatatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCttt > 1:1598816/1‑65 (MQ=255) tatatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTa < 1:1176771/71‑1 (MQ=255) tatatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTa < 1:1990288/71‑1 (MQ=255) tatatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTa < 1:2056677/71‑1 (MQ=255) atatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTa < 1:452900/70‑1 (MQ=255) tatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACgg < 1:3788921/48‑1 (MQ=255) tatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGAt > 1:21074/1‑53 (MQ=255) tatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGAt > 1:2017608/1‑53 (MQ=255) tatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGc < 1:1472120/59‑1 (MQ=255) atTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACgg > 1:1734537/1‑71 (MQ=255) tttGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGtt > 1:418821/1‑48 (MQ=255) tttGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCttt < 1:1879492/60‑1 (MQ=255) tttGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGata > 1:2838542/1‑64 (MQ=255) tttGCCTGGATTATGTTCCAGTTTTTCGATCCAGAACCCCTGACGGTTGATCTTCGCTTTGATATTACgg > 1:310023/1‑70 (MQ=255) ggCCTGGGTTGTGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGa > 1:3579025/2‑48 (MQ=19) ccTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcag < 1:1662314/70‑1 (MQ=255) tGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTg < 1:739414/43‑1 (MQ=255) gATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagag < 1:1117211/71‑1 (MQ=255) aTTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagagc < 1:1152466/71‑1 (MQ=255) tATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagag > 1:1901724/1‑68 (MQ=255) gtGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCagcggagcag > 1:3940202/2‑70 (MQ=255) tGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGga < 1:398089/71‑1 (MQ=255) tGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGga < 1:4049330/71‑1 (MQ=255) tGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGga < 1:2483611/71‑1 (MQ=255) gTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGga < 1:1760336/70‑1 (MQ=255) ttCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCttt < 1:1576478/45‑1 (MQ=25) ccAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAgg > 1:2332628/1‑66 (MQ=255) cAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGa > 1:3175627/1‑44 (MQ=255) aGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGcc < 1:3148770/71‑1 (MQ=255) gTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCttt < 1:2168970/40‑1 (MQ=255) gTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCttt < 1:3742768/40‑1 (MQ=255) gTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCttt < 1:2242600/40‑1 (MQ=255) gTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcagcag < 1:4124993/57‑1 (MQ=255) gTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagag < 1:3257406/59‑1 (MQ=255) gTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGccc < 1:1589916/71‑1 (MQ=255) gTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGccc < 1:2442515/71‑1 (MQ=255) tttttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGata > 1:300331/1‑43 (MQ=255) tttttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcag < 1:3682944/53‑1 (MQ=255) tttttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcag < 1:1551770/53‑1 (MQ=255) tttttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGAc < 1:90109/66‑1 (MQ=255) tttCGATCCAGATCCCCTGACGGTTGATCTTCGCttt > 1:2188643/1‑37 (MQ=255) tttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGat > 1:4131593/1‑40 (MQ=255) tttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTa > 1:976679/1‑44 (MQ=255) tttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTa > 1:3539007/1‑44 (MQ=255) tttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcag < 1:3611347/51‑1 (MQ=255) tttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcag < 1:234736/51‑1 (MQ=255) tttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCAt < 1:2566564/70‑1 (MQ=255) cGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcag > 1:144660/1‑48 (MQ=255) gATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGGGCAGGAGACGCCCATgccg < 1:676450/70‑1 (MQ=255) aTCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcag < 1:1678418/46‑1 (MQ=255) aTCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATaccgac > 1:2805520/1‑71 (MQ=255) ccAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATaccgaccg > 1:1803164/1‑71 (MQ=255) gATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCggg > 1:21813/1‑70 (MQ=255) aTCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcagcag > 1:1853814/1‑43 (MQ=255) tCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcagca > 1:3798517/1‑41 (MQ=255) tCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcagca > 1:1003515/1‑41 (MQ=255) tCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAg < 1:2251038/71‑1 (MQ=255) | CTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAG > minE/1015006‑1015142 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |