| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,015,088 | C→T | 14.7% | K327K (AAG→AAA) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,015,088 | 0 | C | T | 14.7% | 197.4 / 22.2 | 75 | K327K (AAG→AAA) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base C (29/35); new base T (6/5); total (35/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.46e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.90e-01 | |||||||||||
AGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTG > minE/1015020‑1015158 | aGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCtt > 1:3143774/1‑71 (MQ=255) ctTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCt > 1:3407522/1‑71 (MQ=255) ccGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCttt > 1:2997828/1‑70 (MQ=255) ccGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCttt < 1:550704/70‑1 (MQ=255) gATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGatat < 1:1667321/71‑1 (MQ=255) atatatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCttt > 1:1598816/1‑65 (MQ=255) tatatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTa < 1:1990288/71‑1 (MQ=255) tatatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTa < 1:1176771/71‑1 (MQ=255) tatatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTa < 1:2056677/71‑1 (MQ=255) atatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTa < 1:452900/70‑1 (MQ=255) tatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGc < 1:1472120/59‑1 (MQ=255) atTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACgg > 1:1734537/1‑71 (MQ=255) tttGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCttt < 1:1879492/60‑1 (MQ=255) tttGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGata > 1:2838542/1‑64 (MQ=255) tttGCCTGGATTATGTTCCAGTTTTTCGATCCAGAACCCCTGACGGTTGATCTTCGCTTTGATATTACgg > 1:310023/1‑70 (MQ=255) ccTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcag < 1:1662314/70‑1 (MQ=255) gATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagag < 1:1117211/71‑1 (MQ=255) aTTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagagc < 1:1152466/71‑1 (MQ=255) tATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagag > 1:1901724/1‑68 (MQ=255) gtGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCagcggagcag > 1:3940202/2‑70 (MQ=255) tGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGga < 1:398089/71‑1 (MQ=255) tGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGga < 1:4049330/71‑1 (MQ=255) tGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGga < 1:2483611/71‑1 (MQ=255) gTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGga < 1:1760336/70‑1 (MQ=255) ttCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCttt < 1:1576478/45‑1 (MQ=25) ccAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAgg > 1:2332628/1‑66 (MQ=255) cAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGa > 1:3175627/1‑44 (MQ=255) aGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGcc < 1:3148770/71‑1 (MQ=255) gTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCttt < 1:2242600/40‑1 (MQ=255) gTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCttt < 1:2168970/40‑1 (MQ=255) gTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCttt < 1:3742768/40‑1 (MQ=255) gTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcagcag < 1:4124993/57‑1 (MQ=255) gTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagag < 1:3257406/59‑1 (MQ=255) gTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGccc < 1:2442515/71‑1 (MQ=255) gTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGccc < 1:1589916/71‑1 (MQ=255) tttttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGata > 1:300331/1‑43 (MQ=255) tttttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcag < 1:3682944/53‑1 (MQ=255) tttttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcag < 1:1551770/53‑1 (MQ=255) tttttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGAc < 1:90109/66‑1 (MQ=255) tttCGATCCAGATCCCCTGACGGTTGATCTTCGCttt > 1:2188643/1‑37 (MQ=255) tttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGat > 1:4131593/1‑40 (MQ=255) tttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTa > 1:976679/1‑44 (MQ=255) tttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTa > 1:3539007/1‑44 (MQ=255) tttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcag < 1:3611347/51‑1 (MQ=255) tttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcag < 1:234736/51‑1 (MQ=255) tttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCAt < 1:2566564/70‑1 (MQ=255) cGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcag > 1:144660/1‑48 (MQ=255) gATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGGGCAGGAGACGCCCATgccg < 1:676450/70‑1 (MQ=255) aTCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcag < 1:1678418/46‑1 (MQ=255) aTCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATaccgac > 1:2805520/1‑71 (MQ=255) ccAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATaccgaccg > 1:1803164/1‑71 (MQ=255) gATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCggg > 1:21813/1‑70 (MQ=255) aTCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcagcag > 1:1853814/1‑43 (MQ=255) tCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcagca > 1:1003515/1‑41 (MQ=255) tCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcagca > 1:3798517/1‑41 (MQ=255) tCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAg < 1:2251038/71‑1 (MQ=255) tGACGGTTGATCTTCGCTTTGATATTACGGTCagcagagcag < 1:2459745/42‑1 (MQ=255) cgcGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATgcg > 1:2523653/3‑71 (MQ=255) aCGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATgcgc > 1:2693676/1‑71 (MQ=255) aCGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATgc < 1:864029/69‑1 (MQ=255) aCGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATgc < 1:366663/69‑1 (MQ=255) gTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGt > 1:767302/1‑71 (MQ=255) gTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGga < 1:4009767/39‑1 (MQ=255) gTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGt < 1:3655157/71‑1 (MQ=255) gTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGt < 1:2306050/71‑1 (MQ=255) ttGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGt > 1:1126405/1‑70 (MQ=255) ttGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGt > 1:163156/1‑70 (MQ=255) ttGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGt > 1:2901124/1‑70 (MQ=255) ttGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGt > 1:1146590/1‑70 (MQ=255) tGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGcc < 1:2929735/43‑1 (MQ=255) tGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTg > 1:626491/1‑70 (MQ=255) tGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGa > 1:2700594/1‑71 (MQ=255) tGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGa > 1:2511486/1‑71 (MQ=255) tGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGa > 1:2695710/1‑71 (MQ=255) tCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGt > 1:1859979/1‑71 (MQ=255) cTTCGCTTTTATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGa < 1:173596/67‑1 (MQ=255) cTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTg > 1:2658490/1‑71 (MQ=255) cTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTg > 1:2779622/1‑71 (MQ=255) | AGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTG > minE/1015020‑1015158 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |