| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,015,130 | A→G | 24.2% | G313G (GGT→GGC) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,015,130 | 0 | A | G | 24.2% | 111.4 / 35.9 | 62 | G313G (GGT→GGC) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base A (22/25); new base G (7/8); total (29/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCA > minE/1015064‑1015199 | gATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGGGCAGGAGACGCCCATgccg < 1:676450/70‑1 (MQ=255) aTCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATaccgac > 1:2805520/1‑71 (MQ=255) ccAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATaccgaccg > 1:1803164/1‑71 (MQ=255) gATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCggg > 1:21813/1‑70 (MQ=255) tCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAg < 1:2251038/71‑1 (MQ=255) cgcGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATgcg > 1:2523653/3‑71 (MQ=255) aCGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATgcgc > 1:2693676/1‑71 (MQ=255) aCGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATgc < 1:366663/69‑1 (MQ=255) aCGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATgc < 1:864029/69‑1 (MQ=255) gTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGt > 1:767302/1‑71 (MQ=255) gTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGt < 1:3655157/71‑1 (MQ=255) gTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGt < 1:2306050/71‑1 (MQ=255) ttGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGt > 1:163156/1‑70 (MQ=255) ttGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGt > 1:2901124/1‑70 (MQ=255) ttGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGt > 1:1126405/1‑70 (MQ=255) ttGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGt > 1:1146590/1‑70 (MQ=255) tGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTg > 1:626491/1‑70 (MQ=255) tGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGa > 1:2695710/1‑71 (MQ=255) tGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGa > 1:2511486/1‑71 (MQ=255) tGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGa > 1:2700594/1‑71 (MQ=255) ttttCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCggg < 1:3164221/51‑1 (MQ=255) ttttCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCggg < 1:3168649/51‑1 (MQ=255) tCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGt > 1:1859979/1‑71 (MQ=255) tttCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCggg < 1:2070230/51‑1 (MQ=255) cTTCGCTTTTATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGa < 1:173596/67‑1 (MQ=255) cTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTg > 1:2779622/1‑71 (MQ=255) cTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTg > 1:2658490/1‑71 (MQ=255) ttCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATgc < 1:2952554/59‑1 (MQ=255) ttCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTgg > 1:4109445/1‑71 (MQ=255) ttCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTgg > 1:2984807/1‑71 (MQ=255) cGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACg < 1:529128/66‑1 (MQ=255) cGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACg < 1:3364572/66‑1 (MQ=255) cGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTg < 1:771055/68‑1 (MQ=255) cGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTg < 1:2939146/68‑1 (MQ=255) cGCTTTGATATTACGGGCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTg < 1:2031427/68‑1 (MQ=255) gCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCa < 1:209572/70‑1 (MQ=255) tGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCg > 1:835920/1‑70 (MQ=255) tGATATTACGGGCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCg < 1:1151465/70‑1 (MQ=255) gATATTACGGTCAGCAGAGCAGGAGACGCCCATaccgac > 1:1671867/1‑39 (MQ=255) gATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGa < 1:4014703/70‑1 (MQ=255) ttACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAg < 1:3241916/42‑1 (MQ=255) ttACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGa > 1:3993592/1‑44 (MQ=255) ttACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCa < 1:3981594/70‑1 (MQ=255) aCGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGc > 1:3357711/1‑62 (MQ=255) aCGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGc > 1:1534337/1‑62 (MQ=255) aCGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCAcgc > 1:911659/1‑71 (MQ=255) cGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAAcgcg < 1:2736628/71‑1 (MQ=255) ggTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCAcgcg < 1:2175118/70‑1 (MQ=255) agcagagcagGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTg < 1:965196/50‑1 (MQ=255) gcagagcagGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTc < 1:2543808/71‑1 (MQ=255) cggagcagGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCg > 1:1212214/3‑71 (MQ=255) caaagcagGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCg > 1:3985808/4‑71 (MQ=255) agcagGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGt > 1:1554758/1‑69 (MQ=255) gagaCGCCCATGCCGACCGGGCAGGATGCCCCGTGACGTGGCAGACGGATAACGCGAATg < 1:3274067/60‑1 (MQ=255) agaCGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATg < 1:14321/59‑1 (MQ=255) agaCGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCActcg < 1:2696733/55‑1 (MQ=255) gaCGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAg > 1:1770329/1‑70 (MQ=255) cGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTAt > 1:3319399/1‑71 (MQ=255) gCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATg < 1:638669/55‑1 (MQ=255) cccATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTAttt < 1:1809316/71‑1 (MQ=255) cATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGaa > 1:655367/1‑63 (MQ=255) cATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTac < 1:1319905/71‑1 (MQ=255) cATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTac < 1:2589799/71‑1 (MQ=255) aTACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTacc < 1:2001476/71‑1 (MQ=255) tACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCAcgcg < 1:242371/46‑1 (MQ=255) tACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTacca > 1:245509/1‑71 (MQ=255) accgaccgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCAcg > 1:1644411/1‑43 (MQ=255) accgaccgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCg > 1:946243/1‑58 (MQ=255) | GATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCA > minE/1015064‑1015199 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |