| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,015,199 | A→G | 15.1% | G290G (GGT→GGC) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,015,199 | 0 | A | G | 15.1% | 177.3 / 23.8 | 73 | G290G (GGT→GGC) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base A (34/28); new base G (4/7); total (38/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.34e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.39e-01 | |||||||||||
TACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCG > minE/1015129‑1015267 | tACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTacca > 1:245509/1‑71 (MQ=255) ccgaccgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTacca > 1:401627/1‑69 (MQ=255) ccgaccgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTacca > 1:3443266/1‑69 (MQ=255) ccgaccgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTacca > 1:672198/1‑69 (MQ=255) gaccgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTAccacca > 1:3647370/1‑70 (MQ=255) cgGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACt > 1:2804835/1‑71 (MQ=255) cgGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGAGCGAAGTATTTACCGCCAAACt > 1:423132/1‑71 (MQ=255) ggCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTgc > 1:1363658/1‑71 (MQ=255) gCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTgcg > 1:2118624/1‑71 (MQ=255) gCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTgcg > 1:2227199/1‑71 (MQ=255) gCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCaa < 1:2857572/65‑1 (MQ=255) aTGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCAcc > 1:3522643/1‑70 (MQ=255) tGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCAcc > 1:3854277/1‑69 (MQ=255) tGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCa < 1:3799115/71‑1 (MQ=255) tGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGcc > 1:3839792/1‑69 (MQ=255) ccGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGAcc < 1:3186068/71‑1 (MQ=255) gTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGAc > 1:531074/1‑68 (MQ=255) gACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGAcc > 1:2128846/1‑67 (MQ=255) aCGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGAt > 1:320035/1‑71 (MQ=255) aCGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGAcc < 1:2955778/66‑1 (MQ=255) aCGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGAcc < 1:3892899/66‑1 (MQ=255) aCGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGAcc < 1:3379144/66‑1 (MQ=255) aCGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGAcc < 1:2315201/66‑1 (MQ=255) aCGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGAcc < 1:811356/66‑1 (MQ=255) gTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGAttt < 1:1067884/71‑1 (MQ=255) ggCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTg > 1:3628426/1‑71 (MQ=255) gCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTg < 1:2361308/70‑1 (MQ=255) gCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTg < 1:2654329/70‑1 (MQ=255) aGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTgcgc < 1:3627870/71‑1 (MQ=255) ggCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCt < 1:1201057/71‑1 (MQ=255) ggCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCt < 1:3157958/71‑1 (MQ=255) ggCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCt < 1:2869084/71‑1 (MQ=255) gCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCt < 1:3381379/70‑1 (MQ=255) cGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTc < 1:2394728/71‑1 (MQ=255) cGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTc > 1:1767833/1‑71 (MQ=255) cGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCACACCCAGACCAAGATTTTGCGCTTc < 1:360509/71‑1 (MQ=255) gAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGAtt > 1:2044610/1‑61 (MQ=255) aTCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGAt < 1:1137028/71‑1 (MQ=255) tCACGCGGATGTCGTGAGCGAAGTATTTACCACCaaa > 1:18305/1‑37 (MQ=255) tCACGCGGATGTCGTGAGCGAAGTATTTACCACCaaa > 1:1743315/1‑37 (MQ=255) tCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTg > 1:2131783/1‑40 (MQ=255) cACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCa < 1:2163469/43‑1 (MQ=255) aCGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATc > 1:1280967/1‑69 (MQ=255) aCGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCa > 1:1857560/1‑70 (MQ=255) cgGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCaa < 1:4100535/71‑1 (MQ=255) gATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAAt < 1:2250479/70‑1 (MQ=255) gTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGAttt < 1:3256401/49‑1 (MQ=255) gTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGAttt < 1:2915426/49‑1 (MQ=255) tCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTcctg > 1:1487542/1‑68 (MQ=255) tCGTGAGCGAAGTATTTACCACAAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCttt < 1:1919404/71‑1 (MQ=255) tGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCaa < 1:2455580/40‑1 (MQ=255) gAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCaa > 1:1163824/1‑61 (MQ=255) gAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTc > 1:1704624/1‑70 (MQ=255) aGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTc > 1:3123302/1‑51 (MQ=255) gAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATc > 1:2203206/1‑52 (MQ=255) aaGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTCCTGTTCCAgctg < 1:1793654/71‑4 (MQ=255) gTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTc < 1:3955348/63‑1 (MQ=255) gTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTc < 1:3830387/69‑1 (MQ=255) gTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCca > 1:1588141/1‑71 (MQ=255) tATTTACCACCAAACTGCGCACCCAGACCAAGATTTTgcgc > 1:1071623/1‑41 (MQ=255) tATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCg < 1:1431807/45‑1 (MQ=255) tATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCa < 1:717190/49‑1 (MQ=255) tATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCcac < 1:3416954/71‑1 (MQ=255) tATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCcac < 1:3387526/71‑1 (MQ=255) aTTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCttt < 1:964570/58‑1 (MQ=255) aTTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCcaca > 1:1579372/1‑71 (MQ=255) tttACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGAt > 1:865288/1‑45 (MQ=255) tttACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACAt > 1:3581177/1‑71 (MQ=255) tttACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACAt < 1:773049/71‑1 (MQ=255) tttACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACAt > 1:1398539/1‑71 (MQ=255) tttACCACCAAACTGCGCACCAAGACCAAGATTTTGCGCTTCGATCAGc > 1:2485430/1‑49 (MQ=255) tACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTcc > 1:3783762/1‑59 (MQ=255) tACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCa > 1:3472449/1‑60 (MQ=255) tACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCa > 1:952317/1‑60 (MQ=255) tACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAg > 1:3343906/1‑61 (MQ=255) tACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATcg > 1:2168905/1‑71 (MQ=255) tACCACCAAACTGCGCACCCAGACCAAGATTTTGAGCTTCGATCAGCAATTCTTTTTCCa > 1:254772/1‑60 (MQ=255) tACCACAAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCa > 1:116994/1‑48 (MQ=255) ccaccaAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATcgcg > 1:2750842/1‑71 (MQ=255) ccaccaAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATcgc < 1:2035775/70‑1 (MQ=255) accaAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAg < 1:608130/42‑1 (MQ=255) accaAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAg < 1:253724/42‑1 (MQ=255) accaAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTc > 1:3972340/1‑60 (MQ=255) | TACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCG > minE/1015129‑1015267 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |