| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,015,424 | C→T | 6.6% | K215K (AAG→AAA) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,015,424 | 0 | C | T | 6.6% | 192.6 / 3.2 | 60 | K215K (AAG→AAA) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base C (27/29); new base T (2/2); total (29/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01 | |||||||||||
GAAGTTCCACCAATAACGAACGCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACG > minE/1015359‑1015491 | gAAGTTCCACCAATTACGAACGCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTtctc < 1:3128799/71‑1 (MQ=255) aaGTTCCACCAATAACGAACGCAATATGATACGGAGGACAGCCCGCCGTACCCAGCGTGCGCATCTTCTCa < 1:3087562/71‑1 (MQ=255) aGTTCCACCAATAACGAACGCAATATGATACGGAGGACAGGCCGCCGTACCCAGAGTGCGCATCTTCTCac > 1:3902461/1‑70 (MQ=255) gTTCCACCAATAACGAACGCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCAtc > 1:3623426/1‑63 (MQ=255) ttCCACCAATAACGAACGCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTtctc < 1:2404045/67‑1 (MQ=255) caccaATAACGAACGCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCa < 1:4144687/69‑1 (MQ=255) caccaATAACGAACGCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAg < 1:2363718/70‑1 (MQ=255) caATAACGAACGCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTaa > 1:3802509/1‑71 (MQ=255) caATAACGAACGCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTaa > 1:1969830/1‑71 (MQ=255) aTAACGAACGCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTtctc < 1:2516570/59‑1 (MQ=255) aTAACGAACGCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTtctc < 1:2511546/59‑1 (MQ=255) aTAACGAACGCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAAtt > 1:1804315/1‑71 (MQ=255) tAACGAACGCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAAttt < 1:3082614/71‑1 (MQ=255) aacgaacgCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAAtttt > 1:1343309/1‑71 (MQ=255) aacgaacgCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAAttt > 1:1101247/1‑70 (MQ=255) acgaacgCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAAttttt > 1:3431155/1‑71 (MQ=255) acgaacgCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAAttttt > 1:1204964/1‑71 (MQ=255) acgaacgCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAAtt > 1:3298937/1‑68 (MQ=255) gaacgCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAAtttt > 1:3493596/1‑68 (MQ=255) acgCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGt < 1:3465464/71‑1 (MQ=255) cgCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGtt > 1:3433731/1‑71 (MQ=255) cgCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGtt > 1:2433084/1‑71 (MQ=255) aaTATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTc < 1:2656048/64‑1 (MQ=255) tatGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAAttttt < 1:3343770/61‑1 (MQ=255) tatGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTccc > 1:3152333/1‑70 (MQ=255) atGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTc > 1:3197935/1‑67 (MQ=255) tGATACGGAGTACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCgg > 1:1820205/1‑71 (MQ=255) tGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCa > 1:1707942/1‑61 (MQ=255) tGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCgg > 1:564102/1‑71 (MQ=255) gATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGtt < 1:3379125/63‑1 (MQ=255) aTACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCg < 1:1171088/71‑1 (MQ=255) tACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGt < 1:1888421/71‑1 (MQ=255) tACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGt < 1:2422891/71‑1 (MQ=255) tACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGt < 1:3182220/71‑1 (MQ=255) cGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGt > 1:2682675/1‑69 (MQ=255) gaggaCAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAg > 1:1467537/1‑70 (MQ=255) ggcAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCat < 1:1803033/65‑2 (MQ=255) gaCAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGt < 1:1248692/68‑1 (MQ=255) gaCAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGt < 1:3678043/68‑1 (MQ=255) aGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTaa < 1:805717/67‑1 (MQ=255) ggCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCtt > 1:1826154/1‑71 (MQ=255) ggCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCtt < 1:1332529/71‑1 (MQ=255) gccgccGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCttt > 1:3175734/1‑71 (MQ=255) gccgccGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCttt > 1:468997/1‑71 (MQ=255) gccgccGTACCCAGCGTGAGCATCTTCTCAACCAGGTAATTTTTc > 1:3124926/1‑45 (MQ=255) ccgccgTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTg < 1:2762064/71‑1 (MQ=255) ccgTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGtt < 1:4108094/71‑1 (MQ=255) gTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTc < 1:2342474/71‑1 (MQ=255) tACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTc < 1:253518/70‑1 (MQ=255) ccAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTg < 1:876715/70‑1 (MQ=255) cAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCa < 1:1942641/50‑1 (MQ=255) aGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGa > 1:392222/1‑69 (MQ=255) cGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATaga < 1:140574/71‑1 (MQ=255) cGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATaga < 1:1523652/71‑1 (MQ=255) tacgcATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTg > 1:617274/3‑64 (MQ=255) tGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAgaga < 1:14176/71‑1 (MQ=255) tGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAgaga < 1:1466124/71‑1 (MQ=255) cgcATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATa > 1:666274/1‑71 (MQ=255) gcATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATAc > 1:3279619/1‑71 (MQ=255) cATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTc > 1:562725/1‑57 (MQ=255) cATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTc > 1:69817/1‑57 (MQ=255) tcttctCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCt > 1:306130/1‑57 (MQ=255) tcttctCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATagag < 1:2890554/64‑1 (MQ=255) tcttctCAACCAGGTAATTTTTAAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACg > 1:919753/1‑69 (MQ=255) | GAAGTTCCACCAATAACGAACGCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACG > minE/1015359‑1015491 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |