| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 217,996 | A→T | 41.5% | intergenic (‑88/‑18) | yafU ← / → yafF | predicted inner membrane protein/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 217,996 | 0 | A | T | 41.5% | 56.7 / 118.7 | 94 | intergenic (‑88/‑18) | yafU/yafF | predicted inner membrane protein/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (27/28); new base T (21/18); total (48/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.51e-01 | |||||||||||
CTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAAT > minE/217928‑218059 | ctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAg < 1:3776470/70‑1 (MQ=255) gATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCg > 1:326242/1‑71 (MQ=255) aTTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGt > 1:512236/1‑71 (MQ=255) tttAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTc > 1:2025755/1‑71 (MQ=255) tttAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTc < 1:1901136/71‑1 (MQ=255) ttAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCt > 1:496185/1‑71 (MQ=255) ttAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCt > 1:215941/1‑71 (MQ=255) ttAACCGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCt > 1:2440319/1‑71 (MQ=255) aCCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTg < 1:1041706/69‑1 (MQ=255) aCCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGa < 1:1979510/71‑1 (MQ=255) aCCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTCCACAGGCGTCTgg < 1:1929880/70‑1 (MQ=255) cAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTg > 1:3055515/1‑71 (MQ=255) gagaAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGt < 1:2527608/71‑1 (MQ=255) gagaAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGt < 1:2411306/71‑1 (MQ=255) agaAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTc < 1:3086913/60‑1 (MQ=255) agaAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTc > 1:184842/1‑60 (MQ=255) agaAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTa < 1:1513302/71‑1 (MQ=255) gaAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaa > 1:1978075/1‑71 (MQ=255) gaAATTCGCCACAGCGATACGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaa > 1:4088144/1‑71 (MQ=255) aaaGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACg > 1:1383737/2‑65 (MQ=255) aaGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTaa < 1:625707/70‑1 (MQ=255) ttCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatg < 1:198313/69‑1 (MQ=39) ttCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGt < 1:2250256/65‑1 (MQ=255) cGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTaatgaa > 1:1405483/1‑69 (MQ=255) cGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTaatgaa > 1:3391866/1‑69 (MQ=255) gCCACAGCGATCTGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatgaatg < 1:3161061/70‑1 (MQ=255) gCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatgaatg < 1:3823459/70‑1 (MQ=39) gCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTaatgaatg < 1:1720862/70‑1 (MQ=255) gCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTaat < 1:3222011/65‑1 (MQ=255) gCCACAGAAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatgaatg < 1:425924/70‑1 (MQ=255) ccACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAAtgaatgaa < 1:1327535/71‑1 (MQ=255) cacaGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTc > 1:3019099/1‑50 (MQ=255) cacaGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTaatgaatg > 1:2275848/1‑68 (MQ=255) cacaGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTaa > 1:583595/1‑62 (MQ=255) acaGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAga > 1:1267931/1‑71 (MQ=39) acaGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAga > 1:745010/1‑71 (MQ=255) acaGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAga > 1:618963/1‑71 (MQ=255) caGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTg > 1:553838/1‑50 (MQ=255) caGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTaatga > 1:3796999/1‑63 (MQ=255) caGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAgac < 1:46986/71‑1 (MQ=255) caGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAgac < 1:742332/71‑1 (MQ=255) aGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgac > 1:2079355/1‑70 (MQ=255) aGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgac > 1:3847868/1‑70 (MQ=255) aGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgac < 1:3563126/70‑1 (MQ=255) gCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgacga < 1:770845/71‑1 (MQ=255) gaTCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACg < 1:1590271/49‑1 (MQ=255) gaTCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACg < 1:599508/49‑1 (MQ=255) tCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTgg > 1:1520299/1‑51 (MQ=255) tCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTgg > 1:4095779/1‑51 (MQ=255) tCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTaa > 1:2276241/1‑54 (MQ=255) ccGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGt < 1:3402317/51‑1 (MQ=255) ccGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaa > 1:2670122/1‑53 (MQ=255) ccGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGc < 1:3433863/38‑1 (MQ=255) ccGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGc < 1:3298503/38‑1 (MQ=255) cGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAatgaatga > 1:3173880/1‑59 (MQ=38) cGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTg > 1:261192/1‑42 (MQ=255) cGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTg > 1:131304/1‑48 (MQ=255) cGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTg > 1:1762635/1‑48 (MQ=255) cGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTaa > 1:9274/1‑52 (MQ=255) cGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACa > 1:3864236/1‑70 (MQ=255) cGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACa > 1:2686778/1‑70 (MQ=255) aaaTCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatgaatg < 1:2334568/56‑1 (MQ=255) aCCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTaat < 1:3152343/49‑1 (MQ=255) cACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAgacga > 1:1360817/1‑59 (MQ=255) cACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAAt > 1:3404292/1‑68 (MQ=255) aCTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAgacga > 1:3222757/1‑58 (MQ=255) cTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAg > 1:1464668/1‑53 (MQ=255) tGGCACGTGGAGAATAAGCTGCACAGTCGTCTGGACGTGGTaatg < 1:211378/45‑1 (MQ=255) gCACGTGGAGAATAAGCTGCACTGGCGTCTGGAGGTGGTAATTAATGAAgacga < 1:3326906/54‑1 (MQ=255) gCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATaagaag < 1:427443/69‑1 (MQ=255) gCACGTGGAGAATAAGCTGCACAGGCGTCTGGACg < 1:1911939/35‑1 (MQ=255) cACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTaatgaa > 1:1340826/1‑44 (MQ=255) aCGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGa < 1:3254373/71‑1 (MQ=255) cGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGa > 1:868611/1‑70 (MQ=255) tGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAATATaaga < 1:77/62‑1 (MQ=255) tGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTaatg < 1:3415811/38‑1 (MQ=255) ggAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACg > 1:3494015/1‑71 (MQ=255) gagaATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGaaatg > 1:1985133/1‑68 (MQ=255) gaATAAGCTGCACTGGCGTCTGGACGTGGTaatgaatg < 1:3401481/38‑1 (MQ=255) gaATAAGCTGCACAGGCGTCTGGACGTGGTaatga < 1:3400197/35‑1 (MQ=255) gaATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACgcag < 1:1101612/71‑1 (MQ=255) aaTAAGCTGCACTGGCGTCTGGACGTGGTaatgaatg < 1:827227/37‑1 (MQ=255) aaTAAGCTGCACAGGCGTCTGGACGTGGTaatgaa < 1:4109538/35‑1 (MQ=255) aaTAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAAt < 1:2377378/53‑1 (MQ=255) aaTAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACgcag > 1:4056465/1‑70 (MQ=255) aTAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACt < 1:3445503/45‑1 (MQ=255) aTAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAAc < 1:3589637/65‑1 (MQ=255) tAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATaa > 1:233314/1‑53 (MQ=255) tAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACgcag > 1:484529/1‑68 (MQ=255) tAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACgcag > 1:1863877/1‑68 (MQ=255) tAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGcagcag < 1:327962/71‑1 (MQ=255) aaGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGcagcag > 1:3768469/1‑70 (MQ=255) cTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAat > 1:2275791/1‑70 (MQ=255) cTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGcagcag < 1:247099/67‑1 (MQ=255) cactGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGaaa > 1:3968423/1‑56 (MQ=255) cacaGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGa > 1:970008/1‑65 (MQ=255) | CTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAAT > minE/217928‑218059 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |