| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,015,451 | C→G | 10.4% | G206G (GGG→GGC) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,015,451 | 0 | C | G | 10.4% | 191.0 / 10.8 | 67 | G206G (GGG→GGC) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base C (33/27); new base G (4/3); total (37/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01 | |||||||||||
TATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGC > minE/1015384‑1015519 | tatGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTccc > 1:3152333/1‑70 (MQ=255) atGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTc > 1:3197935/1‑67 (MQ=255) tGATACGGAGTACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCgg > 1:1820205/1‑71 (MQ=255) tGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCgg > 1:564102/1‑71 (MQ=255) aTACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCg < 1:1171088/71‑1 (MQ=255) tACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGt < 1:3182220/71‑1 (MQ=255) tACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGt < 1:2422891/71‑1 (MQ=255) tACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGt < 1:1888421/71‑1 (MQ=255) cGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGt > 1:2682675/1‑69 (MQ=255) gaggaCAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAg > 1:1467537/1‑70 (MQ=255) ggcAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCat < 1:1803033/65‑2 (MQ=255) gaCAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGt < 1:1248692/68‑1 (MQ=255) gaCAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGt < 1:3678043/68‑1 (MQ=255) aGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTaa < 1:805717/67‑1 (MQ=255) ggCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCtt > 1:1826154/1‑71 (MQ=255) ggCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCtt < 1:1332529/71‑1 (MQ=255) gccgccGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCttt > 1:3175734/1‑71 (MQ=255) gccgccGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCttt > 1:468997/1‑71 (MQ=255) ccgccgTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTg < 1:2762064/71‑1 (MQ=255) ccgTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGtt < 1:4108094/71‑1 (MQ=255) gTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTc < 1:2342474/71‑1 (MQ=255) tACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTc < 1:253518/70‑1 (MQ=255) ccAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTg < 1:876715/70‑1 (MQ=255) cAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCa < 1:1942641/50‑1 (MQ=255) aGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGa > 1:392222/1‑69 (MQ=255) cGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATaga < 1:140574/71‑1 (MQ=255) cGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATaga < 1:1523652/71‑1 (MQ=255) tacgcATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTg > 1:617274/3‑64 (MQ=255) tGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAgaga < 1:1466124/71‑1 (MQ=255) tGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAgaga < 1:14176/71‑1 (MQ=255) cgcATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATa > 1:666274/1‑71 (MQ=255) gcATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATAc > 1:3279619/1‑71 (MQ=255) cATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTc > 1:562725/1‑57 (MQ=255) cATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTc > 1:69817/1‑57 (MQ=255) tcttctCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCt > 1:306130/1‑57 (MQ=255) tcttctCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATagag < 1:2890554/64‑1 (MQ=255) tcttctCAACCAGGTAATTTTTAAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACg > 1:919753/1‑69 (MQ=255) ttctCGACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCtt > 1:1537136/1‑71 (MQ=255) ttctCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCtt > 1:2054421/1‑71 (MQ=255) ttctCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCtt > 1:3507257/1‑71 (MQ=255) ttctCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCtt > 1:421302/1‑71 (MQ=255) ttctCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCtt > 1:3767792/1‑71 (MQ=255) tctcAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTg > 1:3527936/1‑71 (MQ=255) ctcAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTg < 1:234154/70‑1 (MQ=255) aCCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTg > 1:928060/1‑42 (MQ=255) aCCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGc > 1:2576816/1‑71 (MQ=255) ccAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGcc > 1:3564336/1‑71 (MQ=255) cAGGTAATTTTTCAGTTTCCCCGGTGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGcc > 1:201857/1‑70 (MQ=255) cAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc < 1:3335276/61‑1 (MQ=255) aGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGa < 1:2795270/71‑1 (MQ=255) gTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGa < 1:1526825/46‑1 (MQ=255) aaTTTTTCAGTTTCCCCGGCGTCAGTAGCGCTTTGGt > 1:3311029/1‑37 (MQ=255) aaTTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAacca > 1:2331983/1‑71 (MQ=255) gtttttCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTgg < 1:180025/42‑2 (MQ=16) aTTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGtt < 1:3381044/37‑1 (MQ=255) aTTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAaccac > 1:830267/1‑71 (MQ=255) tttttCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGttgg > 1:800671/1‑59 (MQ=255) tttttCAGTTTCCCCGGCGTCAGTAACGCTTTGGtt < 1:3273351/36‑1 (MQ=255) tttttCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGt < 1:1571339/58‑1 (MQ=255) ttttCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTc > 1:617957/1‑37 (MQ=255) tttCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATa > 1:998649/1‑42 (MQ=255) tttCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAaccaccac > 1:2515483/1‑71 (MQ=255) tttCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAaccacca > 1:2589072/1‑70 (MQ=255) tttCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTACCTGATAGAGATAAGTCt > 1:2293951/1‑53 (MQ=39) aGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCttt > 1:1511918/1‑71 (MQ=255) gTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTg < 1:371532/71‑1 (MQ=255) gTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTg > 1:3868438/1‑71 (MQ=255) ttCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGc > 1:3917992/1‑71 (MQ=255) tCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGc < 1:2411132/70‑1 (MQ=255) | TATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGC > minE/1015384‑1015519 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |