| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 218,030 | A→G | 30.4% | Y6C (TAC→TGC) | yafF → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 218,030 | 0 | A | G | 30.4% | 157.2 / 98.3 | 115 | Y6C (TAC→TGC) | yafF | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (42/38); new base G (21/14); total (63/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.43e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
Only 100 of 115 total aligned reads displayed.
CGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGAC > minE/217963‑218099 | cGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACa > 1:2686778/1‑70 (MQ=255) gCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATaagaag < 1:427443/69‑1 (MQ=255) cGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGa > 1:868611/1‑70 (MQ=255) tGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAATATaaga < 1:77/62‑1 (MQ=255) ggAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACg > 1:3494015/1‑71 (MQ=255) gagaATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGaaatg > 1:1985133/1‑68 (MQ=255) gaATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACgcag < 1:1101612/71‑1 (MQ=255) aaTAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAAt < 1:2377378/53‑1 (MQ=255) aaTAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACgcag > 1:4056465/1‑70 (MQ=255) tAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATaa > 1:233314/1‑53 (MQ=255) tAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACgcag > 1:1863877/1‑68 (MQ=255) tAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACgcag > 1:484529/1‑68 (MQ=255) tAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGcagcag < 1:327962/71‑1 (MQ=255) aaGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGcagcag > 1:3768469/1‑70 (MQ=255) cTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAat > 1:2275791/1‑70 (MQ=255) cTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGcagcag < 1:247099/67‑1 (MQ=255) cactGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGaaa > 1:3968423/1‑56 (MQ=255) cacaGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGa > 1:970008/1‑65 (MQ=255) tggCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAat < 1:2760757/63‑1 (MQ=255) acgCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTAtttt < 1:1275039/68‑1 (MQ=255) gCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGaa < 1:2391668/50‑1 (MQ=255) gCGTCTGGACGTGGTAATGAATGAACACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAg > 1:3999352/1‑71 (MQ=255) cGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAgg < 1:3545393/71‑1 (MQ=255) gTCTGGACGTGGTAATTAATGAAGACGACTACAAAATAAGAAGAGGa < 1:940012/47‑1 (MQ=255) gTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAgg > 1:1300484/1‑70 (MQ=255) tCTGGACGTTGTAATGAATTAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTAtttt < 1:224869/65‑1 (MQ=255) tCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAgg < 1:2561576/45‑1 (MQ=255) tCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTAtttt < 1:2602566/65‑1 (MQ=255) tCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGa < 1:541101/71‑1 (MQ=255) cTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTAtttt < 1:2803704/64‑1 (MQ=255) tGGACGTGGTAATGAATGAAGACGACTACAAAATaagaa > 1:1102258/1‑39 (MQ=255) tGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGaa > 1:1040555/1‑56 (MQ=255) tGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGAt > 1:3594168/1‑70 (MQ=255) tGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATa > 1:422698/1‑71 (MQ=255) tGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATa > 1:1841463/1‑71 (MQ=255) gACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAattatt < 1:3994492/59‑1 (MQ=255) gACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAatt < 1:1584520/56‑1 (MQ=255) gACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATAc < 1:3889417/70‑1 (MQ=255) cGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACgg < 1:3190175/70‑1 (MQ=255) gTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGca < 1:2592846/71‑1 (MQ=255) gTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGca > 1:3332144/1‑71 (MQ=255) ggTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAatt < 1:3949504/51‑1 (MQ=39) gTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAACGCCGCAGAATTATTTTCa > 1:1325497/1‑57 (MQ=255) gTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACAt > 1:1807392/1‑71 (MQ=255) gTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACAt < 1:3399054/71‑1 (MQ=255) tAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTAtttt < 1:535521/54‑1 (MQ=255) tAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTAtttt < 1:1343104/54‑1 (MQ=255) aatgaatgAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAattatt > 1:1194112/1‑51 (MQ=255) ttgaattaaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAgg < 1:3333265/55‑1 (MQ=255) atgaatgaAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGc > 1:3557409/1‑71 (MQ=255) atgaatgaAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGc > 1:3641691/1‑71 (MQ=255) atgaatgaAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGc > 1:1471576/1‑71 (MQ=255) tgaatgaaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGa < 1:3383201/43‑1 (MQ=255) gaatgaaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACAt > 1:2789979/1‑66 (MQ=255) aatgaaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTAtttt > 1:844124/1‑49 (MQ=39) aatgaaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTAtttt > 1:3305832/1‑49 (MQ=39) aatgaaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACAt > 1:2725620/1‑65 (MQ=255) aatgaaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTa > 1:891636/1‑70 (MQ=255) aatgaaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTa > 1:2819341/1‑70 (MQ=255) aatgaaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTAt > 1:247012/1‑71 (MQ=255) aatgaaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTAt > 1:1118129/1‑71 (MQ=255) aatgaaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGa > 1:1713938/1‑55 (MQ=255) aatgaaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACgg > 1:225047/1‑60 (MQ=255) aatgaaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTAt > 1:3609852/1‑71 (MQ=255) aatgaaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTAt > 1:2690485/1‑71 (MQ=255) aatgaaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTAt > 1:2222208/1‑71 (MQ=255) atgaaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGa < 1:1689835/40‑1 (MQ=38) atgaaGACGACTGCAAAATAAGAAGAGGAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTAt > 1:2960934/1‑70 (MQ=255) atgaaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAg < 1:442812/51‑1 (MQ=255) atgaaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGca > 1:629782/1‑61 (MQ=255) tgaaGACGACTGCAAAATAAGAAGAGGAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTAt > 1:884783/1‑69 (MQ=255) tgaaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTAtt < 1:3743650/70‑1 (MQ=255) gaaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAggg < 1:1506492/51‑1 (MQ=255) aaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGcaca > 1:3590872/1‑60 (MQ=255) aaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTaa < 1:1125490/70‑1 (MQ=255) aGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACgg > 1:1926202/1‑55 (MQ=255) aGACGACTGCAAAATAAGAAGAGGAAACGCCGCAGAat < 1:2990962/38‑1 (MQ=37) gacgacTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTa < 1:469087/64‑1 (MQ=255) gacgacTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAatat < 1:3859721/71‑1 (MQ=255) acgacTGCAAAATAAGAAGAGGAAATGCAGCAGAattatt < 1:3100439/40‑1 (MQ=38) cgacTGCAAAATAAGAAGAGGAAATGCAGCAGAATTAtttt > 1:1403203/1‑41 (MQ=38) cgacTGCAAAATAAGAAGAGGAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTATTAATAttt < 1:1071762/71‑1 (MQ=255) gacTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGa > 1:910397/1‑46 (MQ=38) gacTACAAAATAAGTAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGcaca > 1:1207762/1‑55 (MQ=255) gacTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATAtttt < 1:2617868/71‑1 (MQ=255) acTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGAt < 1:2962321/46‑1 (MQ=255) acTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGAt < 1:29086/46‑1 (MQ=255) acTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGca < 1:891890/52‑1 (MQ=255) cTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGcaca > 1:3251094/1‑53 (MQ=255) cTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGcaca > 1:160648/1‑53 (MQ=255) cTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGa > 1:3510357/1‑71 (MQ=255) tACAAAATAAGAAGAGGAAACGCAGCAGAATTAtttt > 1:2306284/1‑37 (MQ=255) tACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAgg < 1:757129/41‑1 (MQ=255) tACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGAt < 1:1523831/44‑1 (MQ=255) tACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATAc < 1:91473/46‑1 (MQ=255) tACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCt > 1:3523939/1‑57 (MQ=255) tACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTa < 1:616644/61‑1 (MQ=255) tACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATAtttt < 1:3558693/68‑1 (MQ=255) tACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGAc > 1:1151283/1‑71 (MQ=255) tACAAAATAAGAAGAGGAAACCCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGAc > 1:3452223/1‑71 (MQ=255) | CGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGAC > minE/217963‑218099 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |