| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,015,640 | A→G | 8.9% | N143N (AAT→AAC) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,015,640 | 0 | A | G | 8.9% | 185.5 / 8.9 | 67 | N143N (AAT→AAC) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base A (29/32); new base G (3/3); total (32/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
CGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCA > minE/1015571‑1015705 | cgcTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGa > 1:1831867/1‑70 (MQ=255) gcTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGAtt < 1:2949303/71‑1 (MQ=255) gcTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGAtt < 1:1557821/71‑1 (MQ=255) ggCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATc > 1:3230298/1‑71 (MQ=255) gTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGatat < 1:592169/70‑1 (MQ=255) gTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGatat < 1:1561873/70‑1 (MQ=255) gTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGatat < 1:2029589/70‑1 (MQ=255) gTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGAtata < 1:4114088/71‑1 (MQ=255) gTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGAtata < 1:329373/71‑1 (MQ=255) tGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGAt > 1:2106549/1‑57 (MQ=255) gCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGa > 1:2795268/1‑55 (MQ=255) gCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGa > 1:2969842/1‑65 (MQ=255) ccGGTGTTGACCTCTTTGTACATGTCCAGCGCCGCATTCTTTGAATAGCGCAGGTTATCTTCGATATAGgt < 1:3211866/71‑1 (MQ=255) ccGGTGTTGACCTCTTTGTACATGTCCAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGata > 1:2358300/1‑66 (MQ=255) ccGGTGTTGACCTCTTTGTACATGTCCAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGata > 1:891835/1‑66 (MQ=255) ccGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTAt > 1:1658889/1‑58 (MQ=255) ccGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAgt < 1:3889355/71‑1 (MQ=255) ccGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAgt < 1:3399309/71‑1 (MQ=255) ccGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAgt > 1:4129317/1‑71 (MQ=255) ccGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAgt < 1:3687280/71‑1 (MQ=255) ggTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAgtgt < 1:3894255/71‑1 (MQ=255) gTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAgtgt > 1:2778410/1‑70 (MQ=255) tATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGa > 1:1052967/1‑50 (MQ=255) tATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTta < 1:3613026/71‑1 (MQ=255) ttCACTTCTTTATACATATCGAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCg < 1:756792/57‑1 (MQ=255) ttCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGa > 1:2574574/1‑48 (MQ=255) ttCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGatat < 1:464675/61‑1 (MQ=255) ttCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAgtgt < 1:3743346/67‑1 (MQ=255) ttCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTtata < 1:1575410/71‑1 (MQ=255) cACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTta > 1:2127447/1‑67 (MQ=255) cACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTta > 1:2090403/1‑67 (MQ=255) cACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGa > 1:2286823/1‑71 (MQ=255) aCTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGac < 1:2048024/71‑1 (MQ=255) cttcttTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCTATATAAgt < 1:3722959/61‑1 (MQ=255) cttcttTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTta < 1:1927646/65‑1 (MQ=255) ttcttTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGa < 1:1069832/53‑1 (MQ=255) ttcttTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAgtgt > 1:63227/1‑62 (MQ=255) cttTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGAt > 1:2928814/1‑42 (MQ=255) cttTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGAt > 1:653114/1‑42 (MQ=255) cttTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGAt > 1:3850327/1‑42 (MQ=255) cttTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATATTCGATATAAGTGTTATAg > 1:1107220/1‑65 (MQ=255) tttATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACcgc > 1:566649/1‑71 (MQ=255) tataCATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGAtt > 1:2825859/1‑40 (MQ=255) tataCATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTAt > 1:1710526/1‑42 (MQ=255) tataCATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACcgcgc > 1:669124/1‑71 (MQ=255) taCATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGAtt < 1:964407/38‑1 (MQ=255) taCATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGAtt < 1:1978949/38‑1 (MQ=255) taCATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCgcgcgc < 1:2887829/71‑1 (MQ=255) taCATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCgcgcgc < 1:1374200/71‑1 (MQ=255) tatCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAgtgt < 1:3827516/51‑1 (MQ=255) tatCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAgc > 1:1916275/1‑71 (MQ=255) atCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCtt > 1:570182/1‑38 (MQ=255) tCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATaa > 1:2666517/1‑45 (MQ=255) tCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTta < 1:1341398/51‑1 (MQ=255) ccAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGcgcc > 1:4017755/1‑71 (MQ=255) cAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGata > 1:3736814/1‑40 (MQ=16) ggcgCGTTTTGCGAGTAGCGCAGATTATCTTCGAtata > 1:3346754/1‑38 (MQ=255) ggcgCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAg > 1:3534261/1‑62 (MQ=255) ccgcaTTCTGTGAATAGCGCAGGTTATCTTCGATATAGGTGtt < 1:227307/42‑1 (MQ=16) cgcgTTTTGCGAGTAGCGCAGATTATCTTCGAtata < 1:2691694/36‑1 (MQ=255) gcgTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTtcatt < 1:3193000/71‑2 (MQ=255) ttttGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATcac > 1:898395/1‑70 (MQ=255) tttGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGa > 1:903594/1‑42 (MQ=255) tttGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATcacca > 1:3572308/1‑71 (MQ=255) cGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATcaccaccac < 1:490723/71‑1 (MQ=255) aGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCg < 1:369095/71‑1 (MQ=255) gTAGCGCAGATTATCTTCGATATAAGTGTTATAGacac > 1:1402853/1‑38 (MQ=255) gTAGCGCAGATTATCTTCGATATAAGTGTTATAGaca < 1:2867547/37‑1 (MQ=255) tAGCGCAGGTTATCTTCGATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGt < 1:3475443/71‑1 (MQ=255) gcgcAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTcc < 1:1394865/71‑1 (MQ=255) gcAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCa > 1:163965/1‑70 (MQ=255) | CGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCA > minE/1015571‑1015705 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |