| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,069,493 | G→A | 17.9% | M130I (ATG→ATA) | cfa → | cyclopropane fatty acyl phospholipid synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,069,493 | 0 | G | A | 17.9% | 186.1 / 31.2 | 78 | M130I (ATG→ATA) | cfa | cyclopropane fatty acyl phospholipid synthase |
| Reads supporting (aligned to +/- strand): ref base G (41/23); new base A (9/5); total (50/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.60e-01 | |||||||||||
GAGTAAAAAACGTGCCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAATCTGGAATCTGCCCAGCA > minE/1069424‑1069561 | gagTAAAAAACGTGCCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGc < 1:1813654/71‑1 (MQ=255)gagTAAAAAACGTGCCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGc > 1:2460552/1‑71 (MQ=255) tAAAAAACGTGCCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCtt > 1:1024703/1‑70 (MQ=255) aaaaaCGTGCCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGAt > 1:198769/1‑71 (MQ=255) aaaCGTGCCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCtt > 1:2679405/1‑66 (MQ=255) aaaCGTGCCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATcc > 1:4105843/1‑71 (MQ=255) aaCGTGCCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATccc > 1:375520/1‑71 (MQ=255) aaCGTGCCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATccc > 1:1704716/1‑71 (MQ=255) aCGTGCCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCt < 1:3418132/71‑1 (MQ=255) cTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGc < 1:3799203/71‑1 (MQ=255) ggATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCaa > 1:1717226/1‑71 (MQ=255) ggATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCaa > 1:3928585/1‑71 (MQ=255) ggATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCa > 1:2265775/1‑70 (MQ=255) gATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGAt > 1:3750442/1‑58 (MQ=255) gATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCaa > 1:1567167/1‑70 (MQ=255) gATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCaa > 1:3438333/1‑70 (MQ=255) gATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCaa > 1:870293/1‑70 (MQ=255) aTAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCaa > 1:1019492/1‑69 (MQ=255) tAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCtt < 1:2835584/53‑1 (MQ=255) tAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCtt < 1:2830949/53‑1 (MQ=255) tAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGAt > 1:1741468/1‑56 (MQ=255) gTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTg < 1:3197453/52‑1 (MQ=255) tCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTcc > 1:2892264/1‑71 (MQ=255) tCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTcc > 1:1020546/1‑71 (MQ=255) ggCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCGGCATGCTTGATCCCTTCAt < 1:550297/59‑1 (MQ=255) ggCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCtt < 1:2898610/56‑1 (MQ=255) ggCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCa > 1:2817048/1‑58 (MQ=255) ggCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTg > 1:884685/1‑71 (MQ=255) gCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGc > 1:2539036/1‑60 (MQ=255) aaaGCGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCATGCAATATTCCTgcgc > 1:2043033/1‑71 (MQ=255) agagCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCt > 1:658093/1‑70 (MQ=255) agagCATTACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCATGCAATAtt < 1:48601/62‑1 (MQ=255) agagCATTACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCATGCAATAtt < 1:2056438/62‑1 (MQ=255) gagCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCTTa > 1:408425/1‑71 (MQ=255) gCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCTTACt > 1:1713244/1‑71 (MQ=255) gCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCTTACt < 1:3193848/71‑1 (MQ=255) cATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCTTACTg > 1:880099/1‑71 (MQ=255) aTTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCTTACTg > 1:94834/1‑70 (MQ=255) ttACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCt < 1:2212272/64‑1 (MQ=255) ttACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGa < 1:4135431/71‑1 (MQ=255) aCGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGAt > 1:1208878/1‑38 (MQ=255) aCGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGAt > 1:2243402/1‑38 (MQ=255) aCGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATcc > 1:195264/1‑40 (MQ=255) aCGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCAt > 1:2434509/1‑46 (MQ=255) aCGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGaa < 1:854542/70‑1 (MQ=255) aCGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGa > 1:2273422/1‑69 (MQ=255) cGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGaaa < 1:3052750/70‑1 (MQ=255) gATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCtt < 1:2507826/61‑1 (MQ=255) gATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGaaa > 1:1050951/1‑69 (MQ=255) gATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGa > 1:1809791/1‑67 (MQ=255) gATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGa > 1:1408941/1‑67 (MQ=255) gATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGa < 1:119908/71‑1 (MQ=255) ttGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGc > 1:313005/1‑71 (MQ=255) tGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCTTa > 1:2354589/1‑58 (MQ=255) gggTAATGACTTGTTCAGCCGCATACTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGcc < 1:3826405/70‑1 (MQ=255) tAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATa < 1:3231190/71‑1 (MQ=255) aaTGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCt < 1:462049/51‑1 (MQ=255) aTGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAAt > 1:1342467/1‑71 (MQ=255) aTGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAAt > 1:677143/1‑71 (MQ=255) aTGACTTGTTCAGCCGCATACTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAAt > 1:3791019/1‑71 (MQ=255) tGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAATc > 1:2173444/1‑71 (MQ=255) tGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAATc < 1:803152/71‑1 (MQ=255) gACTTGTTCAGCCGCATGCTTGATCCCTTCATGCAat > 1:366113/1‑37 (MQ=255) aCTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTc > 1:3706786/1‑40 (MQ=255) aCTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAAt > 1:3882050/1‑68 (MQ=255) aCTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAATCTg > 1:2481163/1‑71 (MQ=255) aCTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAATCTg < 1:846140/71‑1 (MQ=255) aCTTGTTCAGCCGCATGCTTGATCCCTTAATGCAATATTc > 1:2549616/1‑40 (MQ=255) ttGTTCAGACGCATACTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAATCTgg > 1:2147141/1‑70 (MQ=255) gTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAATCTGGa < 1:2193956/69‑1 (MQ=255) gTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAATCTGGa < 1:3633145/69‑1 (MQ=255) gTTCAGCCGCATACTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAATc > 1:3530044/1‑65 (MQ=255) aGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAATCTGGAATCTg < 1:3047708/70‑1 (MQ=255) aGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAATCTGGAATCTg < 1:764922/70‑1 (MQ=255) ccGCATACTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAATCTGGAATCTGcc > 1:822987/1‑70 (MQ=255) ccGCATACTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAATCTGGAATCTGc > 1:1937018/1‑69 (MQ=255) cATGCTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCTATAATCTGGAATCTGCCcagc < 1:2723030/71‑1 (MQ=255) aTGCTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAATCTGGAATCTGCCcagca < 1:3872477/71‑1 (MQ=255) | GAGTAAAAAACGTGCCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAATCTGGAATCTGCCCAGCA > minE/1069424‑1069561 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |