| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,069,791 | G→A | 7.7% | D230N (GAT→AAT) | cfa → | cyclopropane fatty acyl phospholipid synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,069,791 | 0 | G | A | 7.7% | 334.2 / 12.3 | 104 | D230N (GAT→AAT) | cfa | cyclopropane fatty acyl phospholipid synthase |
| Reads supporting (aligned to +/- strand): ref base G (47/49); new base A (3/5); total (50/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.17e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01 | |||||||||||
Only 100 of 106 total aligned reads displayed.
AGGAACGCTGTGAAGGCCTGGATGTCACCATTTTGCTGCAAGATTATCGTGACCTGAACGACCAGTTTGATCGTATTGTTTCTGTGGGGATGTTCGAGCACGTCGGACCGAAAAATTACGATACCTATTTTGCGGTGG > minE/1069723‑1069860 | aGGAACGCTGTGAAGGCCTGGATGTCACCATTTTGCTGCAAGATTATCGTGACCTGAACGACCAGTTTGa < 1:1547182/70‑1 (MQ=255)aGGAACGCTGTGAAGGCCTGGATGTCACCATTTTGCTGCAAGATTATCGCGACCTGAACGACCAGTTTGa < 1:1417522/70‑1 (MQ=255) gAACGCTGTGAAGGCCTGGATGTCACCATTTTGCTGCAAGATTATCGTGACCTGAACGACCAGTTTGATCg > 1:3710761/1‑71 (MQ=255) gAACGCTGTGAAGGCCTGGATGTCACCATTTTGCTGCAAGATTATCGTGACCTGAACGACCAGTTTGATCg < 1:1130552/71‑1 (MQ=255) aaCGCTGTGAAGGCCTGGATGTCACCATTTTGCTGCAAGATTATCGTGACCTGAACGACCAGTTTGATCg > 1:650986/1‑70 (MQ=255) aaCGCTGTGAAGGCCTGGATGTCACCATTTTGCTGCAAGATTATCGTGACCTGAACGACCAGTTTAATCGt < 1:2409046/71‑1 (MQ=255) aaCGCTGTGAAGGCCT‑GATGTCACCATTTTGCTGCAAGATTATCGTGACCTGAACGACCAGTTTGATCGTa < 1:2594264/71‑1 (MQ=255) aCGCTGTGAAGGCCTGGATGTCACCATTTTGCTGCAAGATTATCGTGACCTGAACGACCAGTTTGATCGTa > 1:1945372/1‑71 (MQ=255) aCGCTGTGAAGGCCTGGATGTCACCATTTTGCTGCAAGATTATCGTGACCTGAACGACCAGTTTGATCGTa > 1:835877/1‑71 (MQ=255) cGCTGTGAAGGCCTGGATGTCACCATTTTGCTGCAAGATTATCGTGACCTGAACGACCAGTTTGATCGTAt > 1:1757621/1‑71 (MQ=255) tgAAGGCCTGGATGTCACCATTTTGCTGCAAGATTATCGTGACCTGAACGACCAGTTTGATCGTATTGttt < 1:2817934/71‑1 (MQ=255) aaGGCCTGGATGTCACCATTTTGCTGCAAGATTATCGTGACCTGAACGACCAGTTTGATCGTATTGTTTc > 1:3997153/1‑70 (MQ=255) aaGGCCTGGATGTCACCATTTTGCTGCAAGATTATCGTGACCTGAACGACCAGTTTGATCGTATTGTTTc < 1:718327/70‑1 (MQ=255) aaGGCCTGGATGTCACCATTTTGCTGCAAGATTATCGTGACCTGAACGACCAGTTTGATCGTATTGTTTc < 1:1534660/70‑1 (MQ=255) aaGGCCTGGATGTCACCATTTTGCTGCAAGATTATCGTGACCTGAACGACCAGTTTGATCGTATTGTTTCt > 1:1623490/1‑71 (MQ=255) cTGGATGTCACCATTTTGCTGCAAGATTATCGTGACCTGAACGACCAGTTTGATCGTATTGTTTCTGTggg < 1:2751349/71‑1 (MQ=255) cTGGATGTCACCATTTTGCTGCAAGATTATCGTGACCTGAACGACCAGTTTGATCGTATTGTTTCTGTggg > 1:272160/1‑71 (MQ=255) cTGGATGTCACCATTTTGCTGCAAGATTATCGTGACCTGAACGACCAGTTTGATCGTATTGTTTCTGTggg < 1:471803/71‑1 (MQ=255) cTGGATGTCACCATTTTGCTGCAAGATCATCGTGACCTGAACGACCAGTTTGATCGTATTGTTTCTGTggg < 1:19245/71‑1 (MQ=255) tGGATGTCACCATTTTGCTGCAAGATTATCGTGACCTGAACGACCAGTTTg < 1:3974182/51‑1 (MQ=255) tGGATGTCACCATTTTGCTGCAAGATTATCGTGACCTGAACGACCAGTTTGATCGTATTGTTTCtgt < 1:3728680/67‑1 (MQ=255) gATGTCACCATTTTGCTGCAAGATTATCGTGACCTGAACGACCAGTTTGATCGTATTGTTTCTGTGGGGAt > 1:740601/1‑71 (MQ=255) gATGTCACCATTTTGCTGCAAGATTATCGTGACCTGAACGACCAGTTTGATCGTATTGTTTCTGTGGGGAt > 1:3239298/1‑71 (MQ=255) gATGTCACCATTTTGCTGCAAGATTATCGTGACCTGAACGACCAGTTTGATCGTATTGTTTCTGTGGGGAt > 1:1301817/1‑71 (MQ=255) gATGTCACCATTTTGCTGCAAGATTATCGTGACCTGAACGACCAGTTTGATCGTATTGTTTCTGTGGGGAt > 1:1923251/1‑71 (MQ=255) gATGTCACCATTTTGCTGCAAGATTATCGTGACCTGAACGACCAGTTTAATCGTATTGTTTCTGTGGGGAt > 1:127744/1‑71 (MQ=255) aTGTCACCATTTTGCTGCAAGATTATCGTGACCTGAACGACCAGTTTGATCGTa > 1:3682330/1‑54 (MQ=255) aTGTCACCATTTTGCTGCAAGATTATCGTGACCTGAACGACCAGTTTGATCGTATTGTTTCtgt < 1:3514126/64‑1 (MQ=255) tGTCACCATTTTGCTGCAAGATTATCGTGACCTGAACGACCAGTTTGATCg > 1:405727/1‑51 (MQ=255) gTCACCATTTTGCTGCAAGATTATCGTGACCTGAACGACCAGTTTGAt > 1:938414/1‑48 (MQ=255) gTCACCATTTTGCTGCAAGATTATCGTGACCTGAACGACCAGTTTGATCGTATTGTTTCTGTGGGGATGt < 1:3885361/70‑1 (MQ=255) tCACCATTTTGCTGCAAGATTATCGTGACCTGAACGACCAGTTTGAt > 1:1789114/1‑47 (MQ=255) tCACCATTTTGCTGCAAGATTATCGTGACCTGAACGACCAGTTTGATCGTATTGttt < 1:3128853/57‑1 (MQ=255) tCACCATTTTGCTGCAAGATTATCGTGACCTGAACGACCAGTTTGATCGTATTGttt < 1:3645306/57‑1 (MQ=255) tCACCATTTTGCTGCAAGATTATCGTGACCTGAACGACCAGTTTAATCGTATTGTTTCTGTGGGGATGTTc > 1:2461755/1‑71 (MQ=255) aCCATTTTGCTGCAAGATTATCGTGACCTGAACGACCAGTTTGATCGTATTGTTTc > 1:3918440/1‑56 (MQ=255) aCCATTTTGCTGCAAGATTATCGTGACCTGAACGACCAGTTTGATCGTATTGTTTc > 1:785859/1‑56 (MQ=255) aCCATTTTGCTGCAAGATTATCGTGACCTGAACGACCAGTTTGATCGTATTGTTTCTGTGGGGATGTTc > 1:19288/1‑69 (MQ=255) ccATTTTGCTGCAAGATTATCGTGACCTGAACGACCAGTTTGATCGTATTGTTTCTGTGGGGATGTTCGAg > 1:3362915/1‑71 (MQ=255) ccATTTTGCTGCAAGATTATCGTGACCTGAACGACCAGTTTGATCGTATTGTTTCTGTGGGGATGTTCGAg < 1:3177138/71‑1 (MQ=255) cATTTTGCTGCAAGATTATCGTGACCTGAACGACCAGTTTGATCGTATTGTTTCtgtg < 1:2516270/58‑1 (MQ=255) ttttGCTGCAAGATTATCGTGACCTGAACGACCAGTTTGATCGTATTGTTTCTGTggg > 1:3449843/1‑58 (MQ=255) ttttGCTGCAAGATTATCGTGACCTGAACGACCAGTTTGATCGTATTGTTTCTGTGGGGATGTTCGAGCAc < 1:2225296/71‑1 (MQ=255) ttttGCTGCAAGATTATCGTGACCTGAACGACCAGTTTAATCGt < 1:3012051/44‑1 (MQ=255) tttGCTGCAAGATTATCGTGACCTGAACGACCAGTTTGATCGTATTGTTTCTGTGGGGATGTTc < 1:3300194/64‑1 (MQ=255) tttGCTGCAAGATTATCGTGACCTGAACGACCAGTTTGATCGTATTGTTTCTGTGGGGATGTTGGAGCACg > 1:3301154/1‑71 (MQ=255) tttGCTGCAAGATTATCGTGACCTGAACGACCAGTTTGATCGTATTGTTTCTGTGGGGATGTTCGAGCACg > 1:3156084/1‑71 (MQ=255) ttGCTGCAAGATTATCGTGACCTGAACGACCAGTTTGATCGTATTGTTTCtgtg > 1:2507283/1‑54 (MQ=255) ttGCTGCAAGATTATCGTGACCTGAACGACCAGTTTGATCGTATTGTTTCtg > 1:1612109/1‑52 (MQ=255) ttGCTGCAAGATTATCGTGACCTGAACGACCAGTTTGATCGTATTGTTTCTGTGGGGATGTTCGa > 1:173708/1‑65 (MQ=255) ttGCTGCAAGATTATCGTGACCTGAACGACCAGTTTGATCGTATTGTTTCTGTGGGGATGTTCGAGCACGt > 1:346075/1‑71 (MQ=255) ttGCTGCAAGATTATAGTGACCTGAACGACCAGTTTGATCGTATTGTTTCtg > 1:1028757/1‑52 (MQ=255) ctgcAAGATTATCGTGACCTGAACGACCAGTTTGATCGTATTGTTTCTGTGGGGATGTTCGAGCACGTCg < 1:3682903/70‑1 (MQ=255) tgcAAGATTATCGTGACCTGAACGACCAGTTTGATCGTATTGTTTCTGTGGGGATGTTCGAGCACGTCGGa > 1:1556013/1‑71 (MQ=255) tgcAAGATTATCGTGACCTGAACGACCAGTTTGATCGTATTGTTTCTGTGGGGATGTTCGAGCACGTCGGa > 1:2307123/1‑71 (MQ=255) cAAGATTATCGTGACCTGAACGACCAGTTTGATCGt < 1:1130701/36‑1 (MQ=255) cAAGATTATCGTGACCTGAACGACCAGTTTGATCGt < 1:2095513/36‑1 (MQ=255) cAAGATTATCGTGACCTGAACGACCAGTTTGATCGTATTGTTTCTGTGGGGATGTTCGAGCACGTCGGAcc > 1:4012278/1‑71 (MQ=255) gATTATCGTGACCTGAACGACCAGTTTGATCGTATTg < 1:4066431/37‑1 (MQ=255) gATTATCGTGACCTGAACGACCAGTTTGATCGTATTGTTTc > 1:2379173/1‑41 (MQ=255) gATTATCGTGACCTGAACGACCAGTTTGATCGTATTGTTTCTGTggg < 1:2239845/47‑1 (MQ=255) gATTATCGTGACCTGAACGACCAGTTTGATCGTATTGTTTCTGTGGGGATGTTCGAGCACGTCg < 1:345008/64‑1 (MQ=255) aTTATCGTGACCTGAACGACCAGTTTGATCGTATTGTTTCTGTGGGGATGTTCGAGCACGTCGGACCGaa > 1:3667764/1‑70 (MQ=255) ttATCGTGACCTGAACGACCAGTTTGATCGTATTGTTTCTGTGGGGATGTTCGAGCACGTCGGACCGaaaa < 1:2905416/71‑1 (MQ=255) ttATCGTGACCTGAACGACCAGTTTGATCGTATTGTTTCTGTGGGGATGTTCGAGCACGTCGGACCGaaa < 1:2810089/70‑1 (MQ=255) ttATCGTGACCTGAACGACCAGTTTGATCGTATTGTTTCTGTGGGGATGTTCGAGCACGTCGGACCGaaa < 1:188508/70‑1 (MQ=255) ttATCGTGACCTGAACGACCAGTTTGATCGTATTGTTTCTGTGGGGATGTTCGAGCACGTCGGACCGaaa > 1:684459/1‑70 (MQ=255) aTCGTGCCCTGAACGACCAGTTTGATCGTATTGTTTCtgt < 1:3810046/40‑1 (MQ=255) aTCGTGACCTGAACGACCAGTTTGATCGTATTGTTTCtg > 1:1535193/1‑39 (MQ=255) aTCGTGACCTGAACGACCAGTTTGATCGTATTGTTTCTGTGGGGATGTTCGAGCACGTCg < 1:3965733/60‑1 (MQ=255) tCGTGACCTGAACGACCAGTTTGATCGTATTGTTTCTGTGGGGATGTTCGAGCa > 1:1087628/1‑54 (MQ=255) tCGTGACCTGAACGACCAGTTTGATCGTATTGTTTCTGTGGGGATGTTCGAGCACGTCGGACCGaaaaa > 1:271204/1‑69 (MQ=255) gTGACCTGAACGACCAGTTTGATCGTATTGTTTCTGTgggg < 1:3014228/41‑1 (MQ=255) gTGACCTGAACGACCAGTTTGATCGTATTGTTTCTGTGGGGATGTTCGAGCACGTCGGACCGAAAAATTAc < 1:1538341/71‑1 (MQ=255) gTGACCTGAACGACCAGTTTGATCGTATTGTTTCTGTGGGGATGTTCGAGCACGTCGGACCGAAAAATTAc > 1:455031/1‑71 (MQ=255) gTGACCTGAACGACCAGTTTGATCGTATTGTTTCTGTGGGGATGTTCGAGCACGTCGGACCGAAAAATTAc < 1:752237/71‑1 (MQ=255) gTGACCTGAACGACCAGTTTAATCGTATTGTTTCTGTGGGGATGTTCGAGCACGTCGGACCGAAAAATTAc < 1:2155757/71‑1 (MQ=255) aCCTGAACGACCAGTTTGATCGTATTGTTTCTGTGGGGATGTTCGAGCACGTCGGACCGaaaaa > 1:1839158/1‑64 (MQ=255) ccTGAACGACCAGTTTGATCGTATTGTTTCTGTGGGGATGt < 1:2975706/41‑1 (MQ=255) cTGAACGACCAGTTTGATCGTATTGTTTCTGTGGGGAt < 1:3215297/38‑1 (MQ=255) cTGAACGACCAGTTTGATCGTATTGTTTCTGTGGGGATGt < 1:1057216/40‑1 (MQ=255) tGAACGACCAGTTTGATCGTATTGTTTCTGTGGGGATGTTCGAg < 1:1563155/44‑1 (MQ=255) tGAACGACCAGTTTGATCGTATTGTTTCTGTGGGGATGTTCGAGCACGTCGGACCGaaa < 1:1621436/59‑1 (MQ=255) tGAACGACCAGTTTGATCGTATTGTTTCTGTGGGGATGTTCGAGCACGTCGGACCGAAAAATTACGATAcc > 1:1942000/1‑71 (MQ=255) gAACGACCAGTTTGATCGTATTGTTTCTGTGGGGATGTTCGAGCAc < 1:2908755/46‑1 (MQ=255) gAACGACCAGTTTGATCGTATTGTTTCTGTGGGGATGTTCGAGCACGTCGGACCg > 1:552017/1‑55 (MQ=255) gAACGACCAGTTTAATCGTATTGTTTCTGTGGGGATGTTCGAGCACGTCGGACCGAAAAATTACGATAcc > 1:1675376/1‑70 (MQ=255) aaCGACCAGTTTGATCGTATTGTTTGTGTGGGGAt < 1:1857425/35‑1 (MQ=255) aaCGACCAGTTTGATCGTATTGTTTCTGTGGGGATGTTCGAGCACGTCGGACCGa > 1:75890/1‑55 (MQ=255) aaCGACCAGTTTAATCGTATTGTTTCTGTGGGGATGTTCGAGCACGTCGGACCGAAAAATTACGATAcc < 1:1588916/69‑1 (MQ=255) aaCGACCAGTTTAATCGTATTGTTTCTGTGGGGATGTTCGAGCACGTCGGACCGAAAAATTACGATAcc < 1:787524/69‑1 (MQ=255) aCGACCAGTTTGATCGTATTGTTTCTGTGGGGATGTTCGAGCACGTCGGACCGAAAAATTACGATACCTAt < 1:913659/71‑1 (MQ=255) cAGTTTGATCGTATT‑TTTCTGTGGGGATGTTCGAGCa < 1:931621/37‑1 (MQ=38) tttGATCGTATTGTTTCTGTGGGGATGTTCGAGCAc < 1:749172/36‑1 (MQ=255) tttGATCGTATTGTTTCTGTGGGGATGTTCGAGCACGTCGGACCGAAAAATTACGATACCTATTTTg < 1:1972050/67‑1 (MQ=255) tttGATCGTATTGTTTCTGTGGGGATGTTCGAGCACGTCGGACCGAAAAATTACGATACCTATTTTGCggt < 1:173179/71‑1 (MQ=255) tGATCGTATTGTTTCTGTGGGGATGTTCGAGCACGTCGGACCGAAAAATTACGATACCTATTTTGCggtgg > 1:3282680/1‑71 (MQ=255) tGATCGTATTGTTTCTGTGGGGATGTTCGAGCACGTCGGACCGAAAAATTACGATACCTATTTTGCggtgg > 1:2353552/1‑71 (MQ=255) tGATCGTATTGTTTCTGTGGGGATGTTCGAGCACGTCGGACCGAAAAATTACGATACCTATTTTGCggtgg > 1:2878737/1‑71 (MQ=255) gATCGTATTGTTTCTGTGGGGATGTTCGAGCACGTCGGACCGAAAAAt < 1:1529754/48‑1 (MQ=255) | AGGAACGCTGTGAAGGCCTGGATGTCACCATTTTGCTGCAAGATTATCGTGACCTGAACGACCAGTTTGATCGTATTGTTTCTGTGGGGATGTTCGAGCACGTCGGACCGAAAAATTACGATACCTATTTTGCGGTGG > minE/1069723‑1069860 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |