| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,096,686 | T→C | 8.4% | intergenic (‑310/‑79) | ydiJ ← / → ydiK | predicted FAD‑linked oxidoreductase/predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,096,686 | 0 | T | C | 8.4% | 237.2 / 11.5 | 83 | intergenic (‑310/‑79) | ydiJ/ydiK | predicted FAD‑linked oxidoreductase/predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (49/27); new base C (4/3); total (53/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.99e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01 | |||||||||||
ACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGA > minE/1096618‑1096755 | aCCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTg < 1:2633305/70‑1 (MQ=255) ccACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCt > 1:3689681/1‑71 (MQ=255) caTTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAAT‑GGCCTGTTAAAAACCGCTGAAATTGCtcatc < 1:3643387/71‑1 (MQ=255) aTTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATCGCtcat > 1:2182080/1‑70 (MQ=255) tttttttCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCtcatca > 1:2392273/1‑71 (MQ=255) tttttCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTcatcat > 1:1434131/1‑70 (MQ=255) tttCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTa < 1:2810135/70‑1 (MQ=255) acacGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATCGCTCATCATTa < 1:2369528/64‑1 (MQ=255) acGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGa < 1:1534098/71‑1 (MQ=255) aTCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTcg > 1:3666133/1‑71 (MQ=255) aTCAGAATTAAGGGCAGAATCGGACTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTcg > 1:3006557/1‑71 (MQ=255) tCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCtc > 1:3956783/1‑48 (MQ=255) tCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGt < 1:3204929/62‑1 (MQ=255) tCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGtt < 1:939697/67‑1 (MQ=255) cAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCa < 1:3693088/58‑1 (MQ=255) cAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTcgcg > 1:2175540/1‑71 (MQ=255) aGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTcatcat > 1:900432/1‑51 (MQ=255) aGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAg > 1:1459204/1‑63 (MQ=255) aGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAg > 1:170007/1‑63 (MQ=255) aGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCgt > 1:1364505/1‑71 (MQ=255) aGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCgt > 1:2289513/1‑71 (MQ=255) gAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCgt < 1:1783363/70‑1 (MQ=255) aaTTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCgtgt > 1:2940130/1‑71 (MQ=255) aTTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGtt < 1:1185480/71‑1 (MQ=255) aTTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGtt < 1:520627/71‑1 (MQ=255) aTTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGtt > 1:2150485/1‑71 (MQ=255) aTTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGtt > 1:2348814/1‑71 (MQ=255) ttAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTa > 1:3672938/1‑49 (MQ=255) ttAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGttt > 1:2753352/1‑62 (MQ=255) ttAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGttt > 1:2835383/1‑62 (MQ=255) ttAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGtt > 1:3998820/1‑70 (MQ=255) ttAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGtt > 1:2325232/1‑70 (MQ=255) aaGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCtc > 1:2288808/1‑40 (MQ=255) aaGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTa > 1:1701449/1‑47 (MQ=255) aaGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCAc > 1:3859333/1‑71 (MQ=255) aGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTc < 1:2004112/68‑1 (MQ=255) gggCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCAc > 1:3470784/1‑69 (MQ=255) gggCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCAc > 1:833164/1‑69 (MQ=255) gggCAGAATCCGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTCAGTTTCGCGTGTTCAcg > 1:3466147/1‑70 (MQ=255) gCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCAcgtcg > 1:1429849/1‑71 (MQ=255) gAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCAtt < 1:1232887/39‑1 (MQ=255) gAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCAtt < 1:1413752/39‑1 (MQ=255) gAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCAtt < 1:2376641/39‑1 (MQ=255) aaTCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCAcgtcgcgtc > 1:3296515/1‑71 (MQ=255) aTCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTAt > 1:1991636/1‑39 (MQ=255) aTCGGCCTGTTAAAAACCGCTGAAATTGATCATCATTa > 1:1764621/1‑38 (MQ=255) tCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGc < 1:736662/40‑1 (MQ=255) tCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTcga > 1:3156833/1‑71 (MQ=255) ggCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTcga < 1:2006853/69‑1 (MQ=255) ccTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGAt > 1:89271/1‑71 (MQ=255) ccTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGAt > 1:851596/1‑71 (MQ=255) ccTGTTAAAAACCGCTGAAATCGCTCATCATTATGCAGGTg < 1:2050086/41‑1 (MQ=255) ccTGTTAAAAACCGCTGAAATCGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGAt < 1:3675517/71‑1 (MQ=255) tGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTc < 1:3005358/53‑1 (MQ=255) ttAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTcgcg < 1:2564916/46‑1 (MQ=255) ttAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCAcgtcgcgtc < 1:371599/61‑1 (MQ=255) ttAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCAcgtcgcgtc < 1:895814/61‑1 (MQ=255) ttAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCAcgtcgcgtc < 1:876325/61‑1 (MQ=255) ttAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCAcgtcgcg < 1:2386680/59‑1 (MQ=255) ttAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGAttt < 1:3463587/69‑1 (MQ=255) tAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTcgacga < 1:224998/65‑1 (MQ=255) tAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGAtt > 1:4120988/1‑67 (MQ=255) tAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGa > 1:183363/1‑70 (MQ=255) aaaaaCCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGtt > 1:1536185/1‑48 (MQ=255) aaaaaCCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCAc > 1:1682499/1‑51 (MQ=255) aaaaaCCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCAc > 1:1471996/1‑51 (MQ=255) aaaaaCCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGAc > 1:3419782/1‑70 (MQ=255) aaaaaCCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACg > 1:3125460/1‑71 (MQ=255) aaaaaCCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACg > 1:3548008/1‑71 (MQ=255) aaaaCCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGAtt > 1:2681639/1‑65 (MQ=255) aaaCCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGAttt < 1:518090/65‑1 (MQ=255) aCCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGAt > 1:869979/1‑61 (MQ=255) aCCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGcac > 1:3097940/1‑70 (MQ=255) ccGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGcaca > 1:3014475/1‑70 (MQ=255) ccGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGcaca > 1:2512984/1‑70 (MQ=255) ccGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTAGCGTCGACGAtt > 1:1459336/1‑61 (MQ=255) ccGCTGAAATCGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCAc > 1:1323414/1‑46 (MQ=255) ccGCTGAAATCGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCAc > 1:1752636/1‑46 (MQ=255) agCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCa > 1:3691560/2‑44 (MQ=255) gCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACaaaa < 1:1977755/71‑1 (MQ=255) cTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACaaaaa < 1:857243/71‑1 (MQ=255) cTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACaaaa > 1:2666516/1‑70 (MQ=255) gAAATCGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAg > 1:853150/1‑71 (MQ=255) tGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGa > 1:3324919/1‑70 (MQ=255) | ACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCCTGTTAAAAACCGCTGAAATTGCTCATCATTATGCAGGTGAGTTTCGCGTGTTCACGTCGCGTCGACGATTTGACGCACAAAAAAGGTGA > minE/1096618‑1096755 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |