| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,109,347 | C→T | 20.3% | L88F (CTC→TTC) | ydiS → | predicted oxidoreductase, FAD/NAD(P)‑binding domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,109,347 | 0 | C | T | 20.3% | 129.5 / 26.6 | 59 | L88F (CTC→TTC) | ydiS | predicted oxidoreductase, FAD/NAD(P)‑binding domain |
| Reads supporting (aligned to +/- strand): ref base C (25/22); new base T (8/4); total (33/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.75e-01 | |||||||||||
TCAGCGCCGGTAGAACGCAAGGTCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCCTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACACGCATCTTATACCGTATTGCGTAATCGTCT > minE/1109278‑1109414 | tCAGCGCCGGTAGAACGCAAGGTCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCCt > 1:2348715/1‑71 (MQ=255)tCAGCGCCGGTAGAACGCAAGGTCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCCt < 1:3375275/71‑1 (MQ=255) ccGGTAGAACGCAAGGTCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCCTCGAttt < 1:1434793/71‑1 (MQ=255) ggTAGAACGCAAGGTCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCCTCGAtttt > 1:2061463/1‑70 (MQ=255) ggTAGAACGCAAGGTCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCCTCGAtttt > 1:3706638/1‑70 (MQ=255) aGAACGCAAGGTCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCCTCGATTTTCAcc > 1:2537312/1‑71 (MQ=255) aCGCAAGGTCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCCt > 1:2625885/1‑57 (MQ=255) aCGCAAGGTCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCCTCGATTTTCACcgcg < 1:1192394/71‑1 (MQ=255) cGCAAGGTCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCCTCGATTTTCACcgc > 1:3245355/1‑69 (MQ=255) gCAAGGTCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGATACCCTGGATTTTCACCGCGag > 1:1715726/1‑71 (MQ=255) cAAGGTCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCCTCGATTTTCACCGCGag > 1:2645358/1‑70 (MQ=255) aGGTCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCCTCGATTTTCACCGCGagcag < 1:2480813/71‑1 (MQ=255) ggTCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCCTCGATTTTCACCGCGagcagc > 1:4098260/1‑71 (MQ=255) gTCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCCTCGATTTTCACCGCGAGCAGcc < 1:2760034/71‑1 (MQ=255) tCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCCTCGAtttt > 1:2390123/1‑56 (MQ=255) tCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCCTCGATTTTCACCGCGagcagc > 1:1605134/1‑69 (MQ=255) tCACACCCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCCTCGATTTTCACCGCGAGCAGCCa < 1:1313832/71‑1 (MQ=255) cacaCGCGAGAAATTCTCCTTCTTACCCGAAGATAGCGCCGTTACCTTCGATTTTCACCGCGagcagc > 1:2180143/1‑68 (MQ=255) cacaCGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCCTCGATTTTCACCGCGagcagc > 1:2956266/1‑68 (MQ=255) agaAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCTTCGATTTTCACCGCGAGCAGCCAGAt < 1:2629311/65‑1 (MQ=255) agaAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCTTCGATTTTCACCGCGAGCAGCCAGAt < 1:1216003/65‑1 (MQ=255) agaAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCCTCGATTTTCACCGCGAGCAGCCAGATGTTCCg > 1:681932/1‑71 (MQ=255) gaAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCTTCGATTTTCACCGCGAGCAGCCAGATGTTCCg > 1:2708561/1‑70 (MQ=255) gaAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCCTCGATTTTCACCGCGAGCAGCCAGATGTTCCGc < 1:2110869/71‑1 (MQ=255) aaaaTCTCCTTCTTAACCGAAGAAAGCGCCGTTACCCTCGATTTTCACCGCGAGCAGCCAGAt < 1:2274911/63‑1 (MQ=255) aaaaTCTCCTTCTTAACCGAAGAAAGCGCCGTTACCCTCGATTTTCACCGCGAGCAGCCAGAt < 1:3231397/63‑1 (MQ=255) aaaaTCTCCTTCTTAACCGAAGAAAGCGCCGTTACCCTCGATTTTCACCGCGAGCAGCCAGATGTTc < 1:1013882/67‑1 (MQ=255) aaaTCTCCTTCTTAACCGAAGAAAGCGCCGTTAGCCTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCaa < 1:460339/71‑1 (MQ=255) aaaTCTCCTTCTTAACCGAAGAAAGCGCCGTTACCTTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCaa < 1:3274973/71‑1 (MQ=255) aaTCTCCTTCTTAACCGAAGAAAGCGCCGTTACCCTCGATTTTCACCGCGAGCAGCCAGa > 1:3988180/1‑60 (MQ=255) tctcCTTCTTAACCGAAGAAAGCGCCGTTACCCTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAacac > 1:1443019/1‑71 (MQ=255) tcCTTCTTAACCGAAGAAAGCGCCGTTACCCTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAacac > 1:2215875/1‑69 (MQ=255) cttcttAACCGAAGAAAGCGCCGTTACCCTCGATTTTc > 1:1302458/1‑38 (MQ=255) ttcttAACCGAAGAAAGCGCCGTTACCCTCGATTTTCACcgc < 1:384522/42‑1 (MQ=255) tcttAACCGAAGAAAGCGCCGTTACCTTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCaa > 1:604256/1‑62 (MQ=255) tcttAACCGAAGAAAGCGCCGTTACCCTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCaa < 1:1993678/62‑1 (MQ=255) cttAACCGAAGAAAGCGCCGTTACCTTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACACGCATc < 1:2897168/69‑1 (MQ=255) cttAACCGAAGAAAGCGCCGTTACCCTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACACGCATCt < 1:1012366/70‑1 (MQ=255) ttAACCGAAGAAAGCGCCGTTACCCTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACACGCAt < 1:2521455/67‑1 (MQ=255) tAACCGAAGAAAGCGCCGTTACCTTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACACGCATCTta > 1:552584/1‑70 (MQ=255) tAACCGAAGAAAGCGCCGTTACCTTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACACGCATCTta > 1:699465/1‑70 (MQ=255) aaCCGAAGAAAGCGCCGTTACCCTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACACGCATCt > 1:567321/1‑67 (MQ=255) aaCCGAAGAAAGCGCCGTTACCCTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACACGCATCt > 1:2351605/1‑67 (MQ=255) aaCCGAAGAAAGCGCCGTTACCCTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACACGCATCTta < 1:1739391/69‑1 (MQ=255) aCCGAAGAAAGCGCCGTTACCCTCGATTTTCACCGCGAGCAGCCAg < 1:2213869/46‑1 (MQ=255) gaagaaAGCGCCGTTACCCTCGATTTTCACCGCGAGCAGCCAg < 1:285656/43‑1 (MQ=255) gaagaaAGCGCCGTTACCCTCGATTTTCACCGCGAGCAGCCAGATGTTCCGc < 1:2528898/52‑1 (MQ=255) aagaaAGCGCCGTTACCCTCGATTTTCACCGCGAGCAGCCAGa > 1:1220033/1‑43 (MQ=255) gaaAGCGCCGTTACCCTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACACGCATCTtat < 1:1969776/63‑1 (MQ=255) aaGCGCCGTTACCCTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCaa < 1:3881534/49‑1 (MQ=255) aGCGCCGTTACCCTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACACGCATCTTATACCGTATTGCg > 1:2872269/1‑71 (MQ=255) aGCGCCGTTACCCTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACACGCATCTTATACCGTATTGCg > 1:3886572/1‑71 (MQ=255) aGCGCCGTTACCCTCGATTTTCACCGCGAGCAGCCAGAAGTTCCGCAACACGCATCTTATACCGTATTGCg > 1:3318022/1‑71 (MQ=255) ccGTTACCCTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAac < 1:4054945/45‑1 (MQ=255) gTTACCTTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACACGCATCTTATACCGTATTGCGTAATCg > 1:1324841/1‑71 (MQ=255) gTTACCTTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACACGCATCTTATAACGTATTGCGTAATCg > 1:2891636/1‑71 (MQ=255) ttACCTTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACACg > 1:3383972/1‑45 (MQ=255) tACCCTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACACGCATCTTATACCGTATTGCGTAATCGTc > 1:717436/1‑71 (MQ=255) aCCCTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACACGCATCTTATACCGTATTGCGTAATCGTCt > 1:831354/1‑71 (MQ=255) ccTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACACGCAt < 1:4107891/44‑1 (MQ=255) ccTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACACGCAt < 1:1488796/44‑1 (MQ=255) ccTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACACGCAt < 1:2074388/44‑1 (MQ=255) ccTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACACGCAt < 1:954482/44‑1 (MQ=255) | TCAGCGCCGGTAGAACGCAAGGTCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCCTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACACGCATCTTATACCGTATTGCGTAATCGTCT > minE/1109278‑1109414 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |