| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,202,128 | G→C | 9.2% | T427T (ACG→ACC) | sdaA → | L‑serine deaminase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,202,128 | 0 | G | C | 9.2% | 230.5 / 10.4 | 76 | T427T (ACG→ACC) | sdaA | L‑serine deaminase I |
| Reads supporting (aligned to +/- strand): ref base G (40/29); new base C (3/4); total (43/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01 | |||||||||||
TTAACGCCGCGCGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAA > minE/1202058‑1202196 | ttAACGCCGCGCGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTGGATAAGGTCATCGAAACg < 1:3742065/71‑1 (MQ=255) tAACGCCGCGCGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTGGATAAGGTCATCGAAACGa > 1:2325584/1‑71 (MQ=255) tAACGCCGCGCGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTGGATAAGGTCATCGAAACGa > 1:3721231/1‑71 (MQ=255) tAACGCCGCGCGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTGGATAAGGTCATCGAAACGa > 1:3726082/1‑71 (MQ=255) cgccgcGCGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTGGATAAGGTCATCGAAACGATGt < 1:1091951/71‑1 (MQ=255) ggCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGt > 1:2007158/1‑70 (MQ=255) ctctGCGCCGCACCAGTGCACCGCGCGTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAg < 1:1463917/71‑1 (MQ=255) ctctGCGCCGCACCAGTGCACCGCGCGTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAg < 1:3473038/71‑1 (MQ=255) ctctGCGCCGCACCAGTGCACCGCGCGTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAg > 1:3828835/1‑71 (MQ=255) ctGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAACCATGTACGAAACAGGTAAAGa < 1:712785/71‑1 (MQ=12) ctGCGCCGCACCAGTGCACCGCGCGTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGa > 1:2208185/1‑71 (MQ=255) tGCGCCGCACCAGTGCACCGCGCGTCTCGCTGGATAAGGTCATCGAAACGATg < 1:1377931/53‑1 (MQ=255) cAGTGCACCGCGCGTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCa < 1:2452681/71‑1 (MQ=255) aGTGCACCGCGCGTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCa < 1:1034642/70‑1 (MQ=255) gTGCACCGCGCGTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACAt > 1:2380527/1‑61 (MQ=255) gTGCACCGCGCGTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACAt > 1:2898195/1‑61 (MQ=255) gTGCACCGCGCGTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCaaa < 1:2126291/71‑1 (MQ=255) gTGCACCGCGCGTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCaa > 1:489676/1‑70 (MQ=255) tGCACCGCGCGTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCgg < 1:3799768/51‑1 (MQ=255) cACCGCGCGTCTCGCTGGATAAGGTCATCGAAACGAt > 1:667878/1‑37 (MQ=255) cACCGCGCGTCTCGCTGGATAAGGTCATCGAAACGAt > 1:584241/1‑37 (MQ=255) cACCGCGCGTCTCGCTGGATAAGGTCATCGAAACGAt > 1:3454234/1‑37 (MQ=255) cACCGCGCGTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATAc > 1:2019384/1‑71 (MQ=255) cACCGCGCGTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATAc > 1:1360214/1‑71 (MQ=255) cACCGCGCGTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATAc < 1:2214042/71‑1 (MQ=255) cACCGCGCGTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAAAAc > 1:2187312/1‑71 (MQ=255) ccGCGCGTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATa > 1:2645408/1‑68 (MQ=255) ccGCGCGTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATAcc < 1:3132007/70‑1 (MQ=255) ccGCGCGTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACcg > 1:1763813/1‑71 (MQ=255) cgcgcgTCTCGCTGGATAAGGTCATCGAAACGATg < 1:2738225/35‑1 (MQ=255) gcgcgTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCaa > 1:375368/1‑63 (MQ=255) cgcgTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGcc > 1:326770/1‑60 (MQ=255) cgcgTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGa < 1:2960531/71‑1 (MQ=255) ctcGCTGGATAAGGTCATCGAATCGATGTACGAAACCGGTAAGGa < 1:2101549/45‑1 (MQ=255) ctcGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGa < 1:3929155/45‑1 (MQ=255) ctcGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACa > 1:2464782/1‑47 (MQ=255) cGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCa > 1:3246103/1‑71 (MQ=255) gCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCAc < 1:2278689/71‑1 (MQ=255) cTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCAcg < 1:901792/71‑1 (MQ=255) cTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCAcg < 1:1667805/71‑1 (MQ=255) ggATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGa < 1:4065938/39‑1 (MQ=255) gATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGaa > 1:2266177/1‑44 (MQ=255) gATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCAcgcg < 1:190977/70‑1 (MQ=255) gATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCAcg < 1:3972827/68‑1 (MQ=255) tAAAGTTATCGAAACCATGTACGAAACAGGTAAAGATATGAACGCCAAGTACCGCGAAACCTCTCGCggcg > 1:461654/1‑69 (MQ=9) tAAAGTTATCGAAACCATGTACGAAACAGGTAAAGATATGAACGCCAAGTACCGCGAAACCTCTCGCggcg < 1:382465/71‑3 (MQ=9) aaGGTCATCGAAACGATGTACGAAACCGGTAAGGACATg < 1:355144/39‑1 (MQ=255) aaGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAAc > 1:1684957/1‑59 (MQ=255) aaGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCggtgg > 1:2299572/1‑71 (MQ=255) aGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGaa < 1:1213629/56‑1 (MQ=255) aGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCggtgg < 1:969779/70‑1 (MQ=255) gTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCa < 1:4137919/43‑1 (MQ=255) ttaTCGAAACCATGTACGAAACAGGTAAAGATATGAACGCCa < 1:2694751/40‑1 (MQ=17) ttaTCGAAACCATGTACGAAACAGGTAAAGATATGAACGCCa < 1:310864/40‑1 (MQ=17) tCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTg < 1:2297868/71‑1 (MQ=255) aTCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCt > 1:3159503/1‑68 (MQ=255) tCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCa < 1:3073080/39‑1 (MQ=255) tCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCt > 1:3004455/1‑54 (MQ=255) tCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCAcgc < 1:142465/59‑1 (MQ=255) gAAACGATGTACGAAACCGGTAAGGACATGAACGCCaaa > 1:298657/1‑39 (MQ=255) gAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGaaa > 1:2227986/1‑49 (MQ=255) gAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGaaa > 1:2223297/1‑49 (MQ=255) gAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAAc > 1:2673881/1‑50 (MQ=255) gAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAAt > 1:3157823/1‑71 (MQ=255) gAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAAt > 1:4121969/1‑71 (MQ=255) gAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAAt > 1:3602236/1‑71 (MQ=255) gAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACAGCGAAAc > 1:3177867/1‑50 (MQ=255) gAAACCATGTACGAAACAGGTAAAGATATGAACGcc > 1:1676812/1‑36 (MQ=12) gAAACCATGTACGAAACAGGTAAAGATATGAACGCCAAGTACCGCGAAACCTCTCGCGGCGGCCTGGCAAt > 1:743552/1‑71 (MQ=17) aaCGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCa < 1:2023539/71‑1 (MQ=255) aCGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAAc > 1:2836474/1‑47 (MQ=255) aCGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGc > 1:562780/1‑65 (MQ=255) aCGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCaa < 1:1724198/71‑1 (MQ=255) aCGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCa > 1:3562572/1‑70 (MQ=255) aCGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCa > 1:1993506/1‑70 (MQ=255) cGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCa > 1:4007118/1‑69 (MQ=255) cGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCa > 1:2792915/1‑69 (MQ=255) gATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCt > 1:3024897/1‑47 (MQ=255) | TTAACGCCGCGCGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAA > minE/1202058‑1202196 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |