| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 218,049 | C→T | 26.9% | N12N (AAC→AAT) | yafF → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 218,049 | 0 | C | T | 26.9% | 255.6 / 102.8 | 145 | N12N (AAC→AAT) | yafF | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (53/53); new base T (21/18); total (74/71) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.41e-01 | |||||||||||
Only 100 of 146 total aligned reads displayed.
GGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAG > minE/217980‑218118 | aaTAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACgcag > 1:4056465/1‑70 (MQ=255) aTAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAAc < 1:3589637/65‑1 (MQ=255) tAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACgcag > 1:484529/1‑68 (MQ=255) tAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACgcag > 1:1863877/1‑68 (MQ=255) tAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGcagcag < 1:327962/71‑1 (MQ=255) aaGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGcagcag > 1:3768469/1‑70 (MQ=255) cTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAat > 1:2275791/1‑70 (MQ=255) cacaGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGa > 1:970008/1‑65 (MQ=255) gCGTCTGGACGTGGTAATGAATGAACACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAg > 1:3999352/1‑71 (MQ=255) cGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAgg < 1:3545393/71‑1 (MQ=255) gTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAgg > 1:1300484/1‑70 (MQ=255) tCTGGACGTTGTAATGAATTAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTAtttt < 1:224869/65‑1 (MQ=255) tCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTAtttt < 1:2602566/65‑1 (MQ=255) tCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGa < 1:541101/71‑1 (MQ=255) tGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGaa > 1:1040555/1‑56 (MQ=255) tGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGAt > 1:3594168/1‑70 (MQ=255) tGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATa > 1:422698/1‑71 (MQ=255) gACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAatt < 1:1584520/56‑1 (MQ=255) gACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATAc < 1:3889417/70‑1 (MQ=255) cGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACgg < 1:3190175/70‑1 (MQ=255) gTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGca < 1:2592846/71‑1 (MQ=255) gTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGca > 1:3332144/1‑71 (MQ=255) ggTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAatt < 1:3949504/51‑1 (MQ=39) gTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAACGCCGCAGAATTATTTTCa > 1:1325497/1‑57 (MQ=255) gTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACAt > 1:1807392/1‑71 (MQ=255) gTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACAt < 1:3399054/71‑1 (MQ=255) atgaatgaAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGc > 1:889177/1‑71 (MQ=255) atgaatgaAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGc > 1:3557409/1‑71 (MQ=255) tgaatgaaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATAc < 1:2460343/60‑1 (MQ=255) gaatgaaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACAt > 1:2789979/1‑66 (MQ=255) gaatgaaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTa > 1:1154347/1‑71 (MQ=255) aatgaaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTAtttt > 1:3305832/1‑49 (MQ=39) aatgaaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTAtttt > 1:844124/1‑49 (MQ=39) aatgaaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACAt > 1:2725620/1‑65 (MQ=255) aatgaaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTa > 1:2819341/1‑70 (MQ=255) aatgaaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTAt > 1:247012/1‑71 (MQ=255) aatgaaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTAt > 1:1118129/1‑71 (MQ=255) aatgaaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGa > 1:1713938/1‑55 (MQ=255) aatgaaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTAt > 1:3609852/1‑71 (MQ=255) atgaaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGa < 1:1689835/40‑1 (MQ=38) atgaaGACGACTGCAAAATAAGAAGAGGAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTAt > 1:2960934/1‑70 (MQ=255) atgaaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAg < 1:442812/51‑1 (MQ=255) tgaaGACGACTGCAAAATAAGAAGAGGAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTAt > 1:884783/1‑69 (MQ=255) tgaaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTAtt < 1:3743650/70‑1 (MQ=255) gaaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAggg < 1:1506492/51‑1 (MQ=255) aaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTaa > 1:2244568/1‑70 (MQ=255) aaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTaa < 1:1125490/70‑1 (MQ=255) aGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACgg > 1:1926202/1‑55 (MQ=255) aGACGACTGCAAAATAAGAAGAGGAAACGCCGCAGAATTATTTTAAGGGATACGGCACATCGCTAtt < 1:971237/67‑1 (MQ=255) aGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGca < 1:2834492/57‑1 (MQ=255) acgacTGCAAAATAAGAAGAGGAAATGCAGCAGAattatt < 1:3100439/40‑1 (MQ=38) cgacTGCAAAATAAGAAGAGGAAATGCAGCAGAATTAtttt > 1:1403203/1‑41 (MQ=38) cgacTGCAAAATAAGAAGAGGAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTATTAATAttt < 1:1071762/71‑1 (MQ=255) gacTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGa > 1:910397/1‑46 (MQ=38) gacTACAAAATAAGTAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGcaca > 1:1207762/1‑55 (MQ=255) gacTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATAtttt > 1:4099478/1‑71 (MQ=255) acTACAAAATAAGAAGAGTAAACGCAGCAGAATTATTTTCAGGGAt < 1:267106/46‑1 (MQ=255) acTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGAt < 1:29086/46‑1 (MQ=255) acTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGAt < 1:2962321/46‑1 (MQ=255) acTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGca < 1:891890/52‑1 (MQ=255) cTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGa > 1:3510357/1‑71 (MQ=255) tgcAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGca < 1:3960874/48‑1 (MQ=39) tACAAAATAAGAAGAGGAAACGCAGCAGAATTAtttt > 1:579730/1‑37 (MQ=255) tACAAAATAAGAAGAGGAAACGCAGCAGAATTAtttt > 1:2306284/1‑37 (MQ=255) tACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAgg < 1:757129/41‑1 (MQ=255) tACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTa < 1:616644/61‑1 (MQ=255) tACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGAc > 1:1151283/1‑71 (MQ=255) tACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGAc > 1:1412998/1‑71 (MQ=255) tACAAAATAAGAAGAGGAAACCCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGAc > 1:3452223/1‑71 (MQ=255) cAAAATAAGAAGAGGAAATGCAGCAGAATTAtttt > 1:3559184/1‑35 (MQ=255) cAAAATAAGAAGAGGAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACg > 1:3335661/1‑70 (MQ=255) cAAAATAAGAAGAGGAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACg > 1:1544234/1‑70 (MQ=255) cAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACg > 1:2184564/1‑70 (MQ=255) aaaTAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGa < 1:3898359/39‑1 (MQ=255) aaaTAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAAt > 1:3252562/1‑71 (MQ=255) aTAAGAAGAGGAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTa > 1:1226933/1‑52 (MQ=255) tAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGTTATTaa < 1:3987488/55‑1 (MQ=255) tAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATgag < 1:843026/71‑1 (MQ=255) tAAGAAGAGGAAACGCAGCAGAATAATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAAt > 1:2261194/1‑68 (MQ=255) aagaagAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATgaga < 1:2361289/71‑1 (MQ=255) agaagaGGAAATGCAGCAGAATTATTTTCAGGGATACGGcaca > 1:2637555/1‑43 (MQ=255) agaagaGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATgata < 1:3397028/70‑3 (MQ=255) agaagaGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATgat > 1:2761210/1‑68 (MQ=255) agaagaGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATgaga < 1:3523578/70‑1 (MQ=255) aagaGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAgg < 1:1545557/71‑1 (MQ=255) aagaGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAgg < 1:716141/71‑1 (MQ=255) agagGAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACGAATGATAAgg > 1:1454612/1‑70 (MQ=255) agagGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAgg < 1:4002204/70‑1 (MQ=255) gagGAAACGCAGCAGAATTATTTTCAGGGATACGGCACAt > 1:2418975/1‑40 (MQ=255) gagGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTAt < 1:158657/46‑1 (MQ=255) agGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTCGACGAATGATAAGGTAt > 1:530055/1‑71 (MQ=255) agGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGa < 1:2462396/59‑1 (MQ=255) agGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTAt < 1:1766001/71‑1 (MQ=255) agGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTAt < 1:2711932/71‑1 (MQ=255) ggAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACGAATGATAAGGTAt < 1:1519628/70‑1 (MQ=255) gAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTc < 1:1069743/71‑1 (MQ=255) gAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTc < 1:1858237/71‑1 (MQ=255) aaaTGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTc < 1:2087874/70‑1 (MQ=255) aaaCGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTc > 1:2414334/1‑70 (MQ=255) aaCGCAGAAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAgg < 1:335349/64‑1 (MQ=255) | GGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAG > minE/217980‑218118 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |