| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,211,229 | G→A | 6.5% | F41F (TTC→TTT) | cspC ← | stress protein, member of the CspA‑family |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,211,229 | 0 | G | A | 6.5% | 296.7 / 7.4 | 92 | F41F (TTC→TTT) | cspC | stress protein, member of the CspA‑family |
| Reads supporting (aligned to +/- strand): ref base G (46/39); new base A (2/4); total (48/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.16e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01 | |||||||||||
AGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAG > minE/1211163‑1211298 | aGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGc < 1:1054703/71‑1 (MQ=255)aGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGc < 1:1785718/71‑1 (MQ=255) gCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCAt > 1:2003158/1‑70 (MQ=255) ccGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCAtt < 1:2044171/70‑1 (MQ=255) ccGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCAt < 1:2949841/69‑1 (MQ=255) ccGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTa < 1:2478729/71‑1 (MQ=255) ccGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTa > 1:3907755/1‑71 (MQ=255) ccTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTg > 1:876310/1‑71 (MQ=255) ccTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTg < 1:2079596/71‑1 (MQ=255) tttCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCAt < 1:649687/62‑1 (MQ=255) ttCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGa > 1:561217/1‑70 (MQ=255) tCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGa > 1:226970/1‑69 (MQ=255) tCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGa > 1:2545893/1‑69 (MQ=255) gCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAg < 1:2579163/68‑1 (MQ=255) ccGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGga < 1:3319079/71‑1 (MQ=255) ccGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGga < 1:408955/71‑1 (MQ=255) cGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGgag < 1:832819/71‑1 (MQ=255) tCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTg < 1:3574691/59‑1 (MQ=255) tCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGgag > 1:3493370/1‑69 (MQ=255) cTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGaa > 1:3833388/1‑45 (MQ=255) cTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc > 1:302072/1‑54 (MQ=255) cTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc > 1:762135/1‑54 (MQ=255) cTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAgt < 1:3163020/71‑1 (MQ=255) tGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGATTTTTGaa < 1:353352/44‑1 (MQ=255) tGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGaa < 1:3378913/44‑1 (MQ=255) tGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCa > 1:2110432/1‑48 (MQ=255) tGAATTTAGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCa > 1:1003292/1‑48 (MQ=255) gtgaTTTCGAACTCTACGCGCTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAgtg > 1:591151/4‑71 (MQ=255) gAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTg < 1:3904064/55‑1 (MQ=255) aaTTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAgtg < 1:2205189/69‑1 (MQ=255) aTTTCGAACTCTACGCGCTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCagag < 1:3136989/63‑1 (MQ=255) tttCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACg < 1:2421040/71‑1 (MQ=255) tCGAACTCTACGCGCTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGa < 1:803385/70‑1 (MQ=255) tCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTa > 1:1819752/1‑45 (MQ=255) tCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTa > 1:2886325/1‑45 (MQ=255) tCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCATGGATAGCGGAGAAGTGTACGaa > 1:629314/1‑71 (MQ=255) gAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGc < 1:2778374/38‑1 (MQ=255) aaCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGc < 1:1649423/37‑1 (MQ=255) aaCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAacac < 1:3285844/71‑1 (MQ=255) aaCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAaca > 1:1987656/1‑70 (MQ=255) tCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAacac < 1:2279969/68‑1 (MQ=255) cAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCt > 1:149205/1‑71 (MQ=255) aaCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCAt < 1:998045/35‑1 (MQ=255) aaCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc > 1:3603370/1‑39 (MQ=255) aaCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGaa > 1:1486940/1‑54 (MQ=255) aaCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAacac > 1:2877128/1‑66 (MQ=255) aaCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAaca < 1:2092200/65‑1 (MQ=255) aaCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCtt > 1:2726902/1‑71 (MQ=255) aaCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCtt < 1:1291169/71‑1 (MQ=255) aaCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCtt > 1:3693883/1‑71 (MQ=255) aCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCttt < 1:818930/71‑1 (MQ=255) aCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCttt < 1:3964724/71‑1 (MQ=255) cGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc > 1:666624/1‑37 (MQ=255) cGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc > 1:1666898/1‑37 (MQ=255) cGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc > 1:558073/1‑37 (MQ=255) gTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTa < 1:3536567/56‑1 (MQ=255) gTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgc > 1:3809707/1‑71 (MQ=255) ttCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgct > 1:2979536/1‑71 (MQ=255) tCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAacac > 1:770969/1‑61 (MQ=255) cTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGa > 1:3330496/1‑38 (MQ=255) cTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgctgc > 1:941143/1‑71 (MQ=255) cTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgctg > 1:3447212/1‑70 (MQ=255) cTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgctg < 1:3052052/70‑1 (MQ=255) tGACCTTCGTCCAGAGATTTGTAACCATCGTTCTGGATAGCAGAGAAGTGTACGaa < 1:1278779/56‑1 (MQ=255) tGACCTTCGTCCAGAGATTTGTAACCATCGTTCTGGATAGCAGAGAAGTGTACGaa < 1:1301994/56‑1 (MQ=255) gACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAg > 1:1740228/1‑39 (MQ=255) aCCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCa < 1:3725181/70‑1 (MQ=255) aCCTTCAGCCAGAGTTTT‑AAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCa < 1:2341625/69‑1 (MQ=255) tCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGc > 1:81415/1‑71 (MQ=255) tCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGc > 1:3223482/1‑71 (MQ=255) cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATc > 1:1257206/1‑55 (MQ=255) cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgc > 1:191753/1‑60 (MQ=255) cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGcc > 1:3032835/1‑71 (MQ=255) cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGcc > 1:2272288/1‑71 (MQ=255) cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGcc > 1:2591574/1‑71 (MQ=255) cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGcc > 1:2992955/1‑71 (MQ=255) cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGcc > 1:3071137/1‑71 (MQ=255) cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGc < 1:1122496/70‑1 (MQ=255) cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGc < 1:864049/70‑1 (MQ=255) gCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAgtg < 1:873024/41‑1 (MQ=255) cAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCt > 1:1740751/1‑52 (MQ=255) agagTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGt < 1:436698/71‑1 (MQ=255) agagTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGt < 1:2435086/71‑1 (MQ=255) gagTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGt > 1:3538257/1‑70 (MQ=255) ttttGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATc < 1:1960922/46‑1 (MQ=255) ttttAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAacacgt < 1:477609/45‑3 (MQ=255) ttttAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCtt < 1:2600963/48‑1 (MQ=255) ttGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgct > 1:2327533/1‑50 (MQ=255) ttGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgc > 1:1325835/1‑49 (MQ=255) ttGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgc > 1:2066364/1‑49 (MQ=255) tGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGaa < 1:4144428/70‑1 (MQ=255) tGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAg > 1:1055842/1‑71 (MQ=255) gAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCa < 1:294587/55‑1 (MQ=255) | AGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAG > minE/1211163‑1211298 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |