| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 105,984 | T→A | 5.1% | Y103F (TAC→TTC) | aroP ← | aromatic amino acid transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 105,984 | 0 | T | A | 5.1% | 231.9 / 3.6 | 79 | Y103F (TAC→TTC) | aroP | aromatic amino acid transporter |
| Reads supporting (aligned to +/- strand): ref base T (42/33); new base A (2/2); total (44/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.08e-01 | |||||||||||
GGGATTTCCGGATACCAGAACTGAATGTATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGC > minE/105915‑106053 | gggATTTCCGGATACCAGAACTGAATGTATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTa < 1:3771609/71‑1 (MQ=255)gggATTTCCGGATACCAGAACTGAATGTATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTa < 1:1794481/71‑1 (MQ=255) ggATTTCCGGATACCAGAACTGAATGTATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTa < 1:3707153/70‑1 (MQ=255) aTTTCCGGATACCAGAACTGAATGTATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAg > 1:3642768/1‑71 (MQ=255) tttCCGGATACCAGAACTGAATGTATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGt < 1:762276/71‑1 (MQ=255) tttCCGGATACCAGAACTGAATGTATTTACCCACGGCAGTCAGCTCAGCCATGCCAACTAAAACGTACAg > 1:2258200/1‑70 (MQ=255) ccGGATACCAGAACTGAATGTATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTAc > 1:2371653/1‑70 (MQ=255) ccGGATACCAGAACTGAATGTATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTAc < 1:1621756/70‑1 (MQ=255) tACAAGAACTGAATGTATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTa > 1:78304/1‑64 (MQ=255) aGAACTGAATGTATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACa > 1:332819/1‑57 (MQ=255) aGAACTGAATGTATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCagtagta > 1:1036227/1‑69 (MQ=255) gAACTGAATGTATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCag < 1:4089615/64‑1 (MQ=255) gAACTGAATGTATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTc > 1:1210586/1‑70 (MQ=255) tGAATGTATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTa < 1:382850/50‑1 (MQ=255) aaagTATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTccagccag < 1:3196029/68‑1 (MQ=255) aTGTATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGt < 1:1921153/46‑1 (MQ=255) tGTATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTa < 1:1368323/46‑1 (MQ=255) tGTATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCagag < 1:2176903/71‑1 (MQ=255) gTATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTAc > 1:1701680/1‑46 (MQ=255) gTATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTAc < 1:434380/51‑1 (MQ=255) tATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACa > 1:2311322/1‑46 (MQ=255) tATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACa > 1:1990617/1‑46 (MQ=255) tATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACa > 1:135300/1‑46 (MQ=255) tATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTAc < 1:1434441/50‑1 (MQ=255) tATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGc > 1:1282508/1‑71 (MQ=255) tATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGc > 1:1307945/1‑71 (MQ=255) tATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCAAGCCAGAGGc > 1:3809937/1‑71 (MQ=255) aTTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTa > 1:2168005/1‑43 (MQ=255) tttACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCagta > 1:2671113/1‑54 (MQ=255) tACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACTGTACCCAGTAGTTCCAGCCAGAGGCGaaa > 1:4048448/1‑71 (MQ=255) tACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTAccc > 1:3524614/1‑48 (MQ=255) tACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTAccc > 1:2281605/1‑48 (MQ=255) tACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTAccc > 1:152817/1‑48 (MQ=255) tACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTAccc > 1:157566/1‑48 (MQ=255) tACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCa > 1:1071948/1‑49 (MQ=255) tACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGaaa > 1:1627064/1‑71 (MQ=255) tACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGaa < 1:3544932/70‑1 (MQ=255) tACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGaa < 1:1363538/70‑1 (MQ=255) cccACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTccagc > 1:1188192/1‑58 (MQ=255) ccACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTa < 1:228981/37‑1 (MQ=255) aCGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCa > 1:665807/1‑44 (MQ=255) cGGCAGTCAGCTCAGCCATGGCAAATAAAACGTACAg > 1:2030201/1‑37 (MQ=255) ggCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAATCGGCaa < 1:533082/71‑1 (MQ=255) ggCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCaa < 1:133881/71‑1 (MQ=255) ggCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCaa < 1:2715884/71‑1 (MQ=255) gCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCaa > 1:3114385/1‑70 (MQ=255) ggTCAGCTCTGCCATTCCCACCAGCACGAACATTACCCAGTAGTTccagcca > 1:3868160/2‑52 (MQ=255) ggTCAGCTCTGCCATTCCCACCAGCACGAACATTACCCAGTAGTTccagcca > 1:3834952/2‑52 (MQ=255) aGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAAc < 1:3514581/71‑1 (MQ=255) gTCAGCTCTGCCATTCCCACCAGCACGAACATTACCCAGTAGTTCCAGCCaga < 1:777500/53‑1 (MQ=255) gTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCaaa < 1:2791479/68‑1 (MQ=255) gTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCaaa < 1:385453/68‑1 (MQ=255) tcagctcagcCATGGCAACTAAAACGTACAGTACCCagt < 1:2434190/39‑1 (MQ=255) tcagctcagcCATGGCAACTAAAACGTACAGTACCCAGAAGTTCCAGCCAGAGGCGAAACCGGCaaa < 1:2172625/67‑1 (MQ=255) agctcagcCATGGCAACTAAAACGTACAGTACCCa > 1:140936/1‑35 (MQ=255) agctcagcCATGGCAACTAAAACGTACAGTACCCAGTAGTTc > 1:21301/1‑42 (MQ=255) agctcagcCATGGCAACTAAAACGTACAGTACCCAGTAGTTc > 1:55725/1‑42 (MQ=255) agctcagcCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGcc > 1:1201327/1‑71 (MQ=255) ctcagcCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGcccc < 1:74492/71‑1 (MQ=255) tcagcCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGccc > 1:3515149/1‑69 (MQ=255) agcCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAg > 1:1020786/1‑70 (MQ=255) ccATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCaa < 1:3992337/57‑1 (MQ=255) aTGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTAtt > 1:1199014/1‑70 (MQ=255) tGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCaaa < 1:4004223/55‑1 (MQ=255) tGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCATTAttt > 1:203800/1‑70 (MQ=255) gCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAg > 1:2303741/1‑63 (MQ=255) gCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTtat > 1:596156/1‑70 (MQ=255) gCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTtat > 1:2609106/1‑70 (MQ=255) aaCTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCaa < 1:1822574/50‑1 (MQ=255) aaCTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCaa < 1:3555752/50‑1 (MQ=255) cTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCaa < 1:3610326/48‑1 (MQ=255) tAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCaa < 1:2029129/47‑1 (MQ=255) tAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCaa < 1:2499720/47‑1 (MQ=255) tAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCa > 1:2164309/1‑57 (MQ=255) aaaCGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAg > 1:3323123/1‑71 (MQ=255) caCGAACATTACCCAGTAGTTCCAGCCAGAGAGGAAGCCCGCa < 1:3176807/42‑1 (MQ=16) aaCGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCaaaa > 1:1475965/1‑46 (MQ=255) aCGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTg < 1:106250/71‑1 (MQ=255) aCGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTg < 1:80708/71‑1 (MQ=255) gTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGc > 1:4040760/1‑71 (MQ=255) | GGGATTTCCGGATACCAGAACTGAATGTATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGC > minE/105915‑106053 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |