| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 218,052 | A→C | 8.8% | A13A (GCA→GCC) | yafF → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 218,052 | 0 | A | C | 8.8% | 337.4 / 30.0 | 137 | A13A (GCA→GCC) | yafF | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (61/64); new base C (8/4); total (69/68) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.66e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
Only 100 of 149 total aligned reads displayed.
GAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAG > minE/217983‑218122 | aaTAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACgcag > 1:4056465/1‑70 (MQ=255) tAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACgcag > 1:1863877/1‑68 (MQ=255) tAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACgcag > 1:484529/1‑68 (MQ=255) tAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGcagcag < 1:327962/71‑1 (MQ=255) aaGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGcagcag > 1:3768469/1‑70 (MQ=255) cTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAat > 1:2275791/1‑70 (MQ=255) cTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGcagcag < 1:247099/67‑1 (MQ=255) cacaGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGa > 1:970008/1‑65 (MQ=255) tggCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAat < 1:2760757/63‑1 (MQ=255) gCGTCTGGACGTGGTAATGAATGAACACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAg > 1:3999352/1‑71 (MQ=255) cGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAgg < 1:3545393/71‑1 (MQ=255) gTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAgg > 1:1300484/1‑70 (MQ=255) tCTGGACGTTGTAATGAATTAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTAtttt < 1:224869/65‑1 (MQ=255) cTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTAtttt < 1:2803704/64‑1 (MQ=255) tGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGaa > 1:1040555/1‑56 (MQ=255) tGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATa > 1:1841463/1‑71 (MQ=255) tGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATa > 1:422698/1‑71 (MQ=255) gACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAattatt < 1:3994492/59‑1 (MQ=255) gTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGca < 1:2592846/71‑1 (MQ=255) gTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGca > 1:3332144/1‑71 (MQ=255) ggTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAatt < 1:3949504/51‑1 (MQ=39) gTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAACGCCGCAGAATTATTTTCa > 1:1325497/1‑57 (MQ=255) gTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACAt < 1:3399054/71‑1 (MQ=255) gTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACAt > 1:1807392/1‑71 (MQ=255) tAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTAtttt < 1:535521/54‑1 (MQ=255) tAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTAtttt < 1:1343104/54‑1 (MQ=255) atgaatgaAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGc > 1:889177/1‑71 (MQ=255) atgaatgaAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGc > 1:3557409/1‑71 (MQ=255) atgaatgaAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGc > 1:1471576/1‑71 (MQ=255) atgaatgaAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGc > 1:3641691/1‑71 (MQ=255) tgaatgaaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATAc < 1:2460343/60‑1 (MQ=255) gaatgaaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTa > 1:1154347/1‑71 (MQ=255) aatgaaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTAtttt > 1:844124/1‑49 (MQ=39) aatgaaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTa > 1:2819341/1‑70 (MQ=255) aatgaaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTAt > 1:1118129/1‑71 (MQ=255) aatgaaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGa > 1:1713938/1‑55 (MQ=255) aatgaaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTAt > 1:3609852/1‑71 (MQ=255) aatgaaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTAt > 1:2690485/1‑71 (MQ=255) atgaaGACGACTGCAAAATAAGAAGAGGAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTAt > 1:2960934/1‑70 (MQ=255) tgaaGACGACTGCAAAATAAGAAGAGGAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTAt > 1:884783/1‑69 (MQ=255) aaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTaa > 1:2244568/1‑70 (MQ=255) aaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGcaca > 1:3590872/1‑60 (MQ=255) aaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTaa < 1:1125490/70‑1 (MQ=255) aGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACgg > 1:1926202/1‑55 (MQ=255) aGACGACTGCAAAATAAGAAGAGGAAACGCCGCAGAat < 1:2990962/38‑1 (MQ=37) aGACGACTGCAAAATAAGAAGAGGAAACGCCGCAGAATTATTTTAAGGGATACGGCACATCGCTAtt < 1:971237/67‑1 (MQ=255) gacgacTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTa < 1:469087/64‑1 (MQ=255) gacgacTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAatat < 1:3859721/71‑1 (MQ=255) acgacTGCAAAATAAGAAGAGGAAATGCAGCAGAattatt < 1:3100439/40‑1 (MQ=38) cgacTGCAAAATAAGAAGAGGAAATGCAGCAGAATTAtttt > 1:1403203/1‑41 (MQ=38) cgacTGCAAAATAAGAAGAGGAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTATTAATAttt < 1:1071762/71‑1 (MQ=255) gacTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGa > 1:910397/1‑46 (MQ=38) gacTACAAAATAAGTAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGcaca > 1:1207762/1‑55 (MQ=255) gacTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATAtttt < 1:2617868/71‑1 (MQ=255) acTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGAt < 1:29086/46‑1 (MQ=255) acTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGca < 1:891890/52‑1 (MQ=255) cTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGcaca > 1:3251094/1‑53 (MQ=255) cTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGcaca > 1:160648/1‑53 (MQ=255) cTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGa > 1:3510357/1‑71 (MQ=255) tgcAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGca < 1:3960874/48‑1 (MQ=39) tACAAAATAAGAAGAGGAAACGCAGCAGAATTAtttt > 1:579730/1‑37 (MQ=255) tACAAAATAAGAAGAGGAAACGCAGCAGAATTAtttt > 1:2306284/1‑37 (MQ=255) tACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAgg < 1:757129/41‑1 (MQ=255) tACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGAt < 1:1523831/44‑1 (MQ=255) tACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTa < 1:616644/61‑1 (MQ=255) tACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGAc > 1:1151283/1‑71 (MQ=255) cAAAATAAGAAGAGGAAATGCAGCAGAATTAtttt > 1:3559184/1‑35 (MQ=255) cAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACg < 1:4025700/70‑1 (MQ=255) cAAAATAAGAAGAGGAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACg > 1:3335661/1‑70 (MQ=255) cAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACgg > 1:289175/1‑46 (MQ=255) cAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACg > 1:2184564/1‑70 (MQ=255) aaaTAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGa < 1:3898359/39‑1 (MQ=255) aaaTAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAAt > 1:3252562/1‑71 (MQ=255) aTAAGAAGAGGAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTa > 1:1226933/1‑52 (MQ=255) tAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGca < 1:1715851/43‑1 (MQ=255) tAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATgag < 1:843026/71‑1 (MQ=255) tAAGAAGAGGAAACGCAGCAGAATAATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAAt > 1:2261194/1‑68 (MQ=255) aagaagAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATgaga < 1:2361289/71‑1 (MQ=255) agaagaGGAAATGCAGCAGAATTATTTTCAGGGATACGGcaca > 1:2637555/1‑43 (MQ=255) agaagaGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATgata < 1:3397028/70‑3 (MQ=255) agaagaGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATgat > 1:2761210/1‑68 (MQ=255) agaagaGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATgaga < 1:3523578/70‑1 (MQ=255) aagaGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAgg < 1:716141/71‑1 (MQ=255) aagaGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAgg < 1:1545557/71‑1 (MQ=255) agagGAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACGAATGATAAgg > 1:1454612/1‑70 (MQ=255) agagGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAgg < 1:1554768/70‑1 (MQ=255) gagGAAACGCAGCAGAATTATTTTCAGGGATACGGCACAt > 1:2418975/1‑40 (MQ=255) gagGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTAt < 1:158657/46‑1 (MQ=255) agGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTCGACGAATGATAAGGTAt > 1:530055/1‑71 (MQ=255) agGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGa < 1:2462396/59‑1 (MQ=255) agGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTAt < 1:2711932/71‑1 (MQ=255) ggAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACGAATGATAAGGTAt < 1:1519628/70‑1 (MQ=255) ggAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGat < 1:3280607/59‑2 (MQ=255) gAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTc < 1:1069743/71‑1 (MQ=255) aaaTGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTc < 1:2087874/70‑1 (MQ=255) aaaCGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTc > 1:2414334/1‑70 (MQ=255) aaCGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAgg > 1:4120883/1‑64 (MQ=255) aaCGCAGAAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAgg < 1:335349/64‑1 (MQ=255) agcagAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAAt > 1:3331619/1‑52 (MQ=255) agcagAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAg < 1:3959747/71‑1 (MQ=255) | GAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAG > minE/217983‑218122 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |