| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,216,822 | C→A | 5.6% | P637P (CCG→CCT) | prc ← | carboxy‑terminal protease for penicillin‑binding protein 3 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,216,822 | 0 | C | A | 5.6% | 300.8 / 2.5 | 88 | P637P (CCG→CCT) | prc | carboxy‑terminal protease for penicillin‑binding protein 3 |
| Reads supporting (aligned to +/- strand): ref base C (54/29); new base A (2/3); total (56/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.49e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.21e-01 | |||||||||||
CACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCATTATTCTCTT > minE/1216753‑1216892 | cACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCGGTAGATCATCCAGTTTCTTCAACTCCg > 1:2360117/1‑71 (MQ=255) ccGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCGGTAGATCATCCAGTTTCTTCAACTCCGGt > 1:113797/1‑71 (MQ=255) cGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCGGTAGATCATCCAGTTTCTTCAACTCCGGtt > 1:1363895/1‑71 (MQ=255) gTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCGGTAGATCATCCAGTTTCTTCAACTCCGGttt < 1:2650658/71‑1 (MQ=255) gTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCGGTAGATCATCCAGTTTCTTCAACTCCGGttt < 1:1155949/71‑1 (MQ=255) tcATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTAcc > 1:2226716/1‑71 (MQ=255) tcATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTAc > 1:970846/1‑70 (MQ=255) tcATACAGATAAGGATCCGGCTCCTGGTAATCTTTCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTAcc > 1:4064465/1‑71 (MQ=255) tCCAGATAAGGATCCGGCTCCAGGTAATCTTTCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTACCTTc < 1:1604929/71‑1 (MQ=255) ccAGATAAGGATCCGGCTCCTGGTAATCTTTCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTACCTTcg > 1:78710/1‑71 (MQ=255) ccAGATAAGGATCCGGCTCCTGGTAATCTTTCGGTAGATCATCCAGTTTCTTCAACTCAGGTTTACCTTc > 1:3847565/1‑70 (MQ=255) tAAGGATCCGGCTCCTGGTAATCTTTCGGTAGATCATCCAGTTTCTTCAACTCCGGttt < 1:946584/59‑1 (MQ=255) tAAGGATCCGGCTCCTGGTAATCTTTCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTACCTTCGCGtt < 1:259808/70‑1 (MQ=255) aaGGATCCGGCTCCTGGTAATCTTTCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTACCTTCGCGttt > 1:2793873/1‑70 (MQ=255) aaGGATCCGGCTCCTGGTAATCTTTCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTACCTTCGCGttt > 1:1694726/1‑70 (MQ=255) aGGATCCGGCTCCTGGTAATCTTTCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTa > 1:2486763/1‑70 (MQ=255) ggATCCGGCTCCTGGTAATCTTTCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTa > 1:892913/1‑69 (MQ=255) gCTCCTGGTAATCTTTCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTACCTTcgcg < 1:280670/58‑1 (MQ=255) cTGGTAATCTTTCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTAc > 1:3895548/1‑47 (MQ=255) cTGGTAATCTTTCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGcgttcgtt < 1:1564455/69‑1 (MQ=255) cTGGTAATCTTTCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCgttcgttc < 1:866098/70‑1 (MQ=255) cTGGTAATCTTTCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCa < 1:1246512/71‑1 (MQ=255) cTGGTAATCTTTCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCa < 1:2592601/71‑1 (MQ=255) tGGTAATCTTTCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGc > 1:2121309/1‑61 (MQ=255) gTAATCTTTCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGcgttcgtt > 1:3134516/1‑66 (MQ=255) gTAATCTTTCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCaaa < 1:2199390/70‑1 (MQ=255) gTAATCTTTCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAAc > 1:3362022/1‑71 (MQ=255) gTAATCTTTCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAAc > 1:3968024/1‑71 (MQ=255) tAATCTTTCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAAcg < 1:580919/71‑1 (MQ=255) aaTCTTTCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAAcgc > 1:3930742/1‑71 (MQ=255) aaTCTTTCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAAcgc > 1:1110624/1‑71 (MQ=255) aaTCTTTCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAAcgc > 1:1115275/1‑71 (MQ=255) cTTTCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTaa < 1:3974677/51‑1 (MQ=255) tttCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTa > 1:1068552/1‑49 (MQ=255) tttCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCaaa > 1:27872/1‑64 (MQ=255) tttCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACgcgc > 1:1873397/1‑69 (MQ=255) tCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTa > 1:3218124/1‑35 (MQ=255) tCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGcgttc > 1:3238108/1‑55 (MQ=255) tCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACgcgc < 1:1947579/67‑1 (MQ=255) tCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCa < 1:3052374/69‑1 (MQ=255) ggTAGATCATCCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGAc < 1:1646680/70‑1 (MQ=255) aGATCATCCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGt > 1:411659/1‑71 (MQ=255) gATCATCCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACgcgc < 1:2444940/61‑1 (MQ=255) gATCATCCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTc > 1:4132107/1‑71 (MQ=255) tcatcCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTa < 1:4071102/39‑1 (MQ=255) tcatcCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCg > 1:587311/1‑70 (MQ=255) tcatcCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGc < 1:4121694/71‑1 (MQ=255) catcCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTa > 1:1251299/1‑38 (MQ=255) catcCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCaaa > 1:153709/1‑53 (MQ=255) catcCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAgacgcgacgc > 1:3176532/1‑70 (MQ=255) catcCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGca > 1:1649308/1‑71 (MQ=255) catcCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGca > 1:1618329/1‑71 (MQ=255) catcCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGca > 1:323911/1‑71 (MQ=255) catcCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGc > 1:1574883/1‑70 (MQ=255) atcCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTaaa > 1:4123272/1‑39 (MQ=255) atcCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGca < 1:2659215/70‑1 (MQ=255) ccAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAg > 1:2891457/1‑38 (MQ=255) ccAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGcgttc > 1:73078/1‑43 (MQ=255) ccAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCa < 1:3548886/48‑1 (MQ=255) ccAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGcatca > 1:1457228/1‑71 (MQ=255) ccAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGcatca > 1:2076528/1‑71 (MQ=255) ccAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGc < 1:368664/67‑1 (MQ=255) ccAGTTTCTTCAACTCAGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGAcgc < 1:124933/62‑1 (MQ=255) cAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGa > 1:4117657/1‑58 (MQ=255) cAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGa > 1:1105112/1‑58 (MQ=255) cAGTTTATTCAACTCCGGTTTACCTTCGCGTTTAAAGCgttcgttc > 1:828541/1‑46 (MQ=255) gTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCt > 1:824028/1‑71 (MQ=255) tttCTTCAACTCCGGTTTACCTTCGCGTTTAAAGcgtt < 1:3339781/38‑1 (MQ=255) tttCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTaaaa > 1:1056649/1‑46 (MQ=255) tttCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCtt < 1:309450/71‑1 (MQ=255) tttCTTCAACTCAGGTTTACCTTCGCGTTTAAAGCGTTCGTTCaaa > 1:625127/1‑46 (MQ=255) tcttcAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCtt > 1:904124/1‑69 (MQ=255) tcttcAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTc < 1:687988/70‑1 (MQ=255) tcttcAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCa > 1:1001514/1‑71 (MQ=255) tcttcAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCa > 1:353638/1‑71 (MQ=255) tcttcAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCa > 1:128120/1‑71 (MQ=255) tcttcAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCa > 1:2564361/1‑71 (MQ=255) tcttcAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCa > 1:2035752/1‑71 (MQ=255) ttcAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGa > 1:3951845/1‑51 (MQ=255) tcAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCatta > 1:3474218/1‑71 (MQ=255) tcAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCatt > 1:1704864/1‑70 (MQ=255) cAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCattat > 1:3926712/1‑71 (MQ=255) cAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCatta < 1:2313273/70‑1 (MQ=255) cAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCat < 1:3084279/68‑1 (MQ=255) cAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCat < 1:3856097/68‑1 (MQ=255) cAACTCAGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGACAGACGCGTCGCATCATCTTCat < 1:1840076/68‑1 (MQ=255) cAACTCAGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGACAGACGCGTCGCATCATCTTCat < 1:1835352/68‑1 (MQ=255) tCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGcatca < 1:995824/57‑1 (MQ=255) ccGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGcatcat > 1:1955721/1‑57 (MQ=255) ccGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCATTATtctc > 1:2685296/1‑70 (MQ=255) ccGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCATTATTctct > 1:1397616/1‑71 (MQ=255) ccGGTTTACCTTCGCGTTTAAAGAGTTCGTTCAAACGCGCCAg > 1:662717/1‑43 (MQ=255) cGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCATTATTctct < 1:3109824/70‑1 (MQ=255) cGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCATTATTCTCtt < 1:1366477/71‑1 (MQ=255) | CACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCATTATTCTCTT > minE/1216753‑1216892 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |