| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,250,792 | A→C | 10.7% | L167L (CTT→CTG) | ruvC ← | component of RuvABC resolvasome, endonuclease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,250,792 | 0 | A | C | 10.7% | 199.6 / 21.6 | 87 | L167L (CTT→CTG) | ruvC | component of RuvABC resolvasome, endonuclease |
| Reads supporting (aligned to +/- strand): ref base A (43/34); new base C (3/6); total (47/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
GGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCC > minE/1250728‑1250861 | ggagTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTc < 1:4107577/71‑1 (MQ=255)ggagTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTc < 1:111008/71‑1 (MQ=255) gTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCa < 1:1768415/62‑1 (MQ=255) gTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCa < 1:159332/62‑1 (MQ=255) gAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAg > 1:1192865/1‑67 (MQ=255) gAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCcgc > 1:3227745/1‑71 (MQ=255) gAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCcgc > 1:741962/1‑71 (MQ=255) gAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCcgc > 1:3736001/1‑71 (MQ=255) gAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCcgc > 1:1569508/1‑71 (MQ=255) aaCGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCcgcg < 1:2672223/70‑1 (MQ=255) aaCGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGa > 1:1997451/1‑71 (MQ=255) aCGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGAt > 1:3257605/1‑71 (MQ=255) gCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGt < 1:617147/58‑1 (MQ=255) gCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTc > 1:3966616/1‑71 (MQ=255) gCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTc > 1:4124444/1‑71 (MQ=255) gCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTc > 1:1709582/1‑71 (MQ=255) tCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGc > 1:2727059/1‑70 (MQ=255) cAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCa < 1:2189709/50‑1 (MQ=255) aGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGAtt > 1:2556503/1‑65 (MQ=255) aGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTc < 1:3749612/70‑1 (MQ=255) aGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTc < 1:3744711/70‑1 (MQ=255) aGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTc < 1:588068/70‑1 (MQ=255) aGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTc < 1:4046693/70‑1 (MQ=255) aGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCa < 1:1077414/71‑1 (MQ=255) aGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTC‑C‑AGGTTCAGCCGCGATTCGCTCATc > 1:1060061/1‑71 (MQ=255) cGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCt > 1:1598028/1‑71 (MQ=255) gAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCt > 1:1400935/1‑69 (MQ=255) gAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTg > 1:2557884/1‑70 (MQ=255) aCTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCAt > 1:787920/1‑71 (MQ=255) aCTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCAt > 1:1375457/1‑71 (MQ=255) cTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATc > 1:2381622/1‑71 (MQ=255) gACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTc > 1:3812072/1‑45 (MQ=255) aCCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCg < 1:265575/69‑1 (MQ=255) aCCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCg < 1:2403607/69‑1 (MQ=255) aCCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCa > 1:3853015/1‑71 (MQ=255) ccGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCg < 1:515561/68‑1 (MQ=255) ccGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCAt < 1:1112308/71‑1 (MQ=255) aGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGa < 1:3170835/48‑1 (MQ=255) aGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCt < 1:1453051/71‑1 (MQ=255) aGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCt < 1:2329219/71‑1 (MQ=255) aGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCt < 1:2003373/71‑1 (MQ=255) aGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCt < 1:3141658/71‑1 (MQ=255) gTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCa < 1:669357/51‑1 (MQ=255) tataACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCt < 1:1018588/48‑1 (MQ=255) tataACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCt < 1:2951190/48‑1 (MQ=255) tataACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCa < 1:984760/61‑1 (MQ=255) taACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGc > 1:585727/1‑45 (MQ=255) taACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACt < 1:2644459/67‑1 (MQ=255) taACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAAc > 1:703777/1‑70 (MQ=255) aaCTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGc < 1:4081060/44‑1 (MQ=255) aaCTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTc > 1:71207/1‑61 (MQ=255) aCTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTg > 1:3720310/1‑71 (MQ=255) aCTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCa > 1:3411022/1‑52 (MQ=255) aCTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCa > 1:141833/1‑52 (MQ=255) aCTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGt < 1:4041046/70‑1 (MQ=255) aCTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTg > 1:1620406/1‑71 (MQ=255) cTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGc > 1:1892124/1‑42 (MQ=255) cTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGc > 1:4033553/1‑42 (MQ=255) cTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGc > 1:2488096/1‑42 (MQ=255) cTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCt > 1:3281834/1‑43 (MQ=255) tAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTgg > 1:1194775/1‑69 (MQ=255) accGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGc < 1:3713282/67‑1 (MQ=255) aCGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCAtt < 1:4019884/54‑1 (MQ=255) aCGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCAtt < 1:1273242/54‑1 (MQ=255) aCGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGt < 1:2491451/71‑1 (MQ=255) aCGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGt < 1:635735/71‑1 (MQ=255) cGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGc < 1:2363333/50‑1 (MQ=255) aGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTc > 1:2384880/1‑36 (MQ=255) gTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAgtgtgt > 1:2199626/1‑70 (MQ=255) tCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGa < 1:796711/71‑1 (MQ=255) cGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGa > 1:2347600/1‑70 (MQ=255) cGCCCTCTCGCAAGGTTCAGCCGAGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGc < 1:3522616/61‑1 (MQ=255) gCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGa > 1:428815/1‑50 (MQ=255) ccTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGc < 1:2773746/36‑1 (MQ=255) ccTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCg > 1:2984584/1‑40 (MQ=255) ccTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCg > 1:2731396/1‑40 (MQ=255) ccTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCAtt > 1:3673434/1‑44 (MQ=255) ccTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGc < 1:3424438/71‑1 (MQ=255) ctctCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCa < 1:1174511/71‑1 (MQ=255) tctcGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAAc < 1:3787786/51‑1 (MQ=255) tctcGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCt < 1:1631965/44‑1 (MQ=255) ctcGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCAtt > 1:120664/1‑41 (MQ=255) tcGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATc > 1:1682510/1‑71 (MQ=255) tcGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAAt > 1:570804/1‑70 (MQ=255) tcGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAAt > 1:269434/1‑70 (MQ=255) tcGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAAt > 1:3059097/1‑70 (MQ=255) tcGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATc > 1:185305/1‑71 (MQ=255) tcGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATc > 1:2618886/1‑71 (MQ=255) gCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGc < 1:4038014/71‑1 (MQ=255) cAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGcc < 1:929415/71‑1 (MQ=255) | GGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCC > minE/1250728‑1250861 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |