| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,256,681 | G→A | 7.1% | E22K (GAA→AAA) | yecO → | predicted methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,256,681 | 0 | G | A | 7.1% | 178.0 / 3.3 | 56 | E22K (GAA→AAA) | yecO | predicted methyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (27/25); new base A (2/2); total (29/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01 | |||||||||||
TATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCGTACCCGGCTATTCCAATATTATTTC > minE/1256617‑1256748 | tATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACggg > 1:1180772/1‑71 (MQ=255) aTGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACggg > 1:1985181/1‑70 (MQ=255) ccACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTg < 1:39778/70‑1 (MQ=255) cACCGCGACAGGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACgg < 1:2443449/63‑1 (MQ=255) cACCGCGACACGCTATTTTCTGCCCATATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTg > 1:2103497/1‑70 (MQ=255) aCCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTg < 1:4081437/69‑1 (MQ=255) aCCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGa < 1:938640/70‑1 (MQ=255) aCCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATAAACGGGTAGCTg > 1:1878899/1‑69 (MQ=255) gACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATAAACGGGTAGCTGAAGTCt < 1:2428511/70‑1 (MQ=255) acGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTccc > 1:970242/1‑71 (MQ=255) ttttCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATAAACGGGTAGCTGAAGTCTTCCCGGat < 1:3961218/69‑1 (MQ=255) ttCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGaa > 1:4130394/1‑43 (MQ=255) ttCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGCCTTCCCGGATATg < 1:1113993/70‑1 (MQ=255) tCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGa < 1:3780241/70‑1 (MQ=255) cTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATc > 1:1430035/1‑71 (MQ=255) tGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACgg < 1:1987322/43‑1 (MQ=255) tGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATAAACGGGTAGCTGAAGTCTTCCCGGATATGATc > 1:3461507/1‑70 (MQ=255) cTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGt > 1:3021824/1‑71 (MQ=255) cTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGt > 1:3893368/1‑71 (MQ=255) tATCGCCAGACTGGGCGACTGGACCTTTGATGAAc < 1:885526/35‑1 (MQ=255) tATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGt < 1:2435302/39‑1 (MQ=255) tATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGt < 1:2484062/39‑1 (MQ=255) aTCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGaa < 1:1272815/45‑1 (MQ=255) aTCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGaa < 1:1165227/45‑1 (MQ=255) tCGCCAGACTGTGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAg < 1:2555172/65‑1 (MQ=255) tCGCCAGACTGGGCGACTGGACCTTTGATGAACggg > 1:3547177/1‑36 (MQ=255) tCGCCAGACTGGGCGACTGGACCTTTGATGAACggg > 1:1580958/1‑36 (MQ=255) tCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGa > 1:596854/1‑43 (MQ=255) tCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTc > 1:1063469/1‑50 (MQ=255) tCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTcc > 1:420519/1‑71 (MQ=255) cGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCg > 1:3822151/1‑71 (MQ=255) gCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCg < 1:2193810/51‑1 (MQ=255) gCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCg > 1:2650434/1‑70 (MQ=255) gCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCg > 1:2991810/1‑70 (MQ=255) cAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGat < 1:1983480/52‑1 (MQ=255) gACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCGTAccc > 1:2881014/1‑71 (MQ=255) aCTGGGCGACTGGACCTTTGATGAACGGGTAGCTGa > 1:2582094/1‑36 (MQ=255) aCTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAg < 1:3799885/38‑1 (MQ=255) aCTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCg < 1:475614/46‑1 (MQ=255) tGGGCGACTGGACCTTTGATGAACGGGTAGCTGaa > 1:1966309/1‑35 (MQ=255) ggCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCGTACCCGGc < 1:2615551/69‑1 (MQ=255) ggCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCGTACCCGGCt < 1:1189594/70‑1 (MQ=255) cGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCGTACCCGGc < 1:2295989/67‑1 (MQ=255) aCTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCGTACCCGGCTATTcc < 1:238252/71‑1 (MQ=255) aCTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCGTACCCGGCTATTc > 1:1045371/1‑70 (MQ=255) tGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCGTACCCGGc < 1:3075019/63‑1 (MQ=255) gACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCGt > 1:73987/1‑54 (MQ=255) gACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCGTACCCGGCTATTCCaa < 1:3320229/69‑1 (MQ=255) aCCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCTTACCCGGCTATTCCAatat < 1:3623449/71‑1 (MQ=255) aCCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCGTACCCGGCTATTCCAata > 1:3846403/1‑70 (MQ=255) ccTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCGTACCCGGCTATTCCAAtatt < 1:637733/71‑1 (MQ=255) cTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCGTACCCGGCTATTCCAAtatta > 1:4133576/1‑71 (MQ=255) tttGATGAACGGGTAGCTGAAGTCTTCCCGGatat > 1:168812/1‑35 (MQ=255) ttGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCGTACCCGGCTATTCCAATattatt > 1:338623/1‑71 (MQ=255) ttGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCGTACCCGGCTATTCCAATattatt > 1:4114666/1‑71 (MQ=255) ttGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCGTACCCGGCTATTCCAATattatt > 1:1043597/1‑71 (MQ=255) gatgaACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCGTACCCGGCTATTCCAATATTATTTc > 1:2348756/1‑71 (MQ=255) | TATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCGTACCCGGCTATTCCAATATTATTTC > minE/1256617‑1256748 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |