| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,259,799 | T→C | 10.2% | L375L (TTA→TTG) | torZ ← | trimethylamine N‑oxide reductase system III, catalytic subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,259,799 | 0 | T | C | 10.2% | 161.2 / 9.5 | 59 | L375L (TTA→TTG) | torZ | trimethylamine N‑oxide reductase system III, catalytic subunit |
| Reads supporting (aligned to +/- strand): ref base T (27/26); new base C (2/4); total (29/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.29e-01 | |||||||||||
GCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAG > minE/1259729‑1259868 | gcgTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCt < 1:2445320/71‑1 (MQ=255) tggggTTACCACCATTGGCAAAATGGTAAGAAAGACCAAAACCGCCGCCGGGTGTGCCGATTTGCCCCaa < 1:3521322/68‑1 (MQ=255) cGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACa > 1:2400682/1‑71 (MQ=255) gCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATcgcc < 1:3819074/70‑1 (MQ=255) gCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATcgcc > 1:2857677/1‑70 (MQ=255) aCCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCTGTGTACCAATCTGCCCTAACATcgccgc > 1:4054426/1‑69 (MQ=255) aCCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCa < 1:2978550/71‑1 (MQ=255) aCCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCa < 1:177382/71‑1 (MQ=255) cGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGc > 1:2220293/1‑71 (MQ=255) gTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAAc > 1:494220/1‑58 (MQ=255) ttGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGt < 1:1597373/71‑1 (MQ=255) gAGTAATTATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCa < 1:475474/70‑1 (MQ=255) aaTGGTAAGAAAGACCAAAACCGCCGCCGGGTGTGCCGATTTGCCCCAACATTGCTGCCAGCGTGAcgat > 1:3313564/1‑67 (MQ=255) aTGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCa > 1:2585453/1‑71 (MQ=255) tAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTa < 1:1611531/43‑1 (MQ=255) tAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATc > 1:3670793/1‑69 (MQ=255) tAAGAAAGACCAAAACCGCCGCCGGGTGTGCCGATTTGCCCCAACATTGCTGCCAGCGTGAcga < 1:1886895/64‑3 (MQ=255) aaCCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATcgccgc < 1:2095070/46‑1 (MQ=255) aCCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCt > 1:1320390/1‑71 (MQ=255) ccAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCgccgcc < 1:1370525/45‑1 (MQ=255) ccAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCgccgcc < 1:638572/45‑1 (MQ=255) ccAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGt < 1:2427535/50‑1 (MQ=255) cAAAACCGCCGCCGGGTGTGCCGATTTGCCCCAACATTGCTGCCAGCGTGACGATCATCCAGTGTTTTTgc < 1:2528450/71‑2 (MQ=255) aaaaaCCGCCGCCCGGTGTACCAATCTGGCCTAACATCGCCGCCAGc < 1:3523507/46‑1 (MQ=255) aaaaCCGCCGCCCGGTGTACCAATCTGCCCTAACATCgccgcc > 1:937192/1‑43 (MQ=255) aaCCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCa < 1:3617065/71‑1 (MQ=255) gccgccCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCa < 1:1517962/70‑1 (MQ=255) cGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCt > 1:1710474/1‑71 (MQ=255) cGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCt < 1:3016714/71‑1 (MQ=255) ggTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTg < 1:3907139/56‑1 (MQ=255) ggTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTg < 1:1351023/56‑1 (MQ=255) gtgtACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCa > 1:1072496/1‑62 (MQ=255) tgtgCCGATTTGCCCCAACATTGCTGCCAGCGTGACGATCATCCAGTGTTTTTGCTCACCAAACTGTTGgc < 1:2356310/71‑1 (MQ=255) gtACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTg > 1:2074946/1‑53 (MQ=255) gtACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCata > 1:2754179/1‑62 (MQ=255) ccAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGttt > 1:1906000/1‑47 (MQ=255) ccAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTg > 1:4101501/1‑62 (MQ=255) ccAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGc > 1:86129/1‑63 (MQ=255) cAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTg < 1:841231/70‑1 (MQ=255) aaTCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGtt < 1:3706085/44‑1 (MQ=255) aTCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAAt < 1:3498550/71‑1 (MQ=255) aTCTGCCCTAACATCGCCCCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTCGCGCTGa > 1:2035281/1‑69 (MQ=255) ttGCCCCAACATTGCTGCCAGCGTGACGATCATCCAGTGTTTTTGCTCACCAAACTGTTGGCGCTGCAttg > 1:3651372/2‑70 (MQ=255) cTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGtt > 1:1286498/1‑47 (MQ=255) cTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTc > 1:949744/1‑71 (MQ=255) tGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGttt > 1:611931/1‑41 (MQ=255) tGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGttt > 1:1722870/1‑41 (MQ=255) tGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTc > 1:3817044/1‑42 (MQ=255) tGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTc > 1:2043001/1‑42 (MQ=255) tGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGgc < 1:1924226/61‑1 (MQ=255) tGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAAtt < 1:3921336/69‑1 (MQ=255) tGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTcc > 1:489801/1‑71 (MQ=255) tGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTcc > 1:676311/1‑71 (MQ=255) tGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTc > 1:2951145/1‑70 (MQ=255) cccTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTc < 1:1958911/68‑1 (MQ=255) ccTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCa < 1:2132639/71‑1 (MQ=255) ccTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCa < 1:1306150/71‑1 (MQ=255) cTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAg < 1:620954/71‑1 (MQ=255) cTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAg < 1:825555/71‑1 (MQ=255) cTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAg > 1:917326/1‑71 (MQ=255) | GCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAG > minE/1259729‑1259868 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |