| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,259,817 | C→G | 10.6% | V369V (GTG→GTC) | torZ ← | trimethylamine N‑oxide reductase system III, catalytic subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,259,817 | 0 | C | G | 10.6% | 187.7 / 11.1 | 66 | V369V (GTG→GTC) | torZ | trimethylamine N‑oxide reductase system III, catalytic subunit |
| Reads supporting (aligned to +/- strand): ref base C (34/25); new base G (3/4); total (37/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01 | |||||||||||
GAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTG > minE/1259749‑1259884 | gAGTAATTATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCa < 1:475474/70‑1 (MQ=255) aaTGGTAAGAAAGACCAAAACCGCCGCCGGGTGTGCCGATTTGCCCCAACATTGCTGCCAGCGTGAcgat > 1:3313564/1‑67 (MQ=255) aTGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCa > 1:2585453/1‑71 (MQ=255) tAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATc > 1:3670793/1‑69 (MQ=255) tAAGAAAGACCAAAACCGCCGCCGGGTGTGCCGATTTGCCCCAACATTGCTGCCAGCGTGAcga < 1:1886895/64‑3 (MQ=255) aCCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCt > 1:1320390/1‑71 (MQ=255) cAAAACCGCCGCCGGGTGTGCCGATTTGCCCCAACATTGCTGCCAGCGTGACGATCATCCAGTGTTTTTgc < 1:2528450/71‑2 (MQ=255) aaCCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCa < 1:3617065/71‑1 (MQ=255) gccgccCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCa < 1:1517962/70‑1 (MQ=255) cGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCt < 1:3016714/71‑1 (MQ=255) cGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCt > 1:1710474/1‑71 (MQ=255) ggTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTg < 1:3907139/56‑1 (MQ=255) ggTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTg < 1:1351023/56‑1 (MQ=255) gtgtACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCa > 1:1072496/1‑62 (MQ=255) tgtgCCGATTTGCCCCAACATTGCTGCCAGCGTGACGATCATCCAGTGTTTTTGCTCACCAAACTGTTGgc < 1:2356310/71‑1 (MQ=255) gtACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTg > 1:2074946/1‑53 (MQ=255) gtACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCata > 1:2754179/1‑62 (MQ=255) ccAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGttt > 1:1906000/1‑47 (MQ=255) ccAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTg > 1:4101501/1‑62 (MQ=255) ccAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGc > 1:86129/1‑63 (MQ=255) cAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTg < 1:841231/70‑1 (MQ=255) aaTCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGtt < 1:3706085/44‑1 (MQ=255) aTCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAAt < 1:3498550/71‑1 (MQ=255) aTCTGCCCTAACATCGCCCCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTCGCGCTGa > 1:2035281/1‑69 (MQ=255) ttGCCCCAACATTGCTGCCAGCGTGACGATCATCCAGTGTTTTTGCTCACCAAACTGTTGGCGCTGCAttg > 1:3651372/2‑70 (MQ=255) cTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGtt > 1:1286498/1‑47 (MQ=255) cTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTc > 1:949744/1‑71 (MQ=255) tGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGttt > 1:611931/1‑41 (MQ=255) tGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGttt > 1:1722870/1‑41 (MQ=255) tGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTc > 1:2043001/1‑42 (MQ=255) tGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTc > 1:3817044/1‑42 (MQ=255) tGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGgc < 1:1924226/61‑1 (MQ=255) tGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAAtt < 1:3921336/69‑1 (MQ=255) tGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTcc > 1:489801/1‑71 (MQ=255) tGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTcc > 1:676311/1‑71 (MQ=255) tGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTc > 1:2951145/1‑70 (MQ=255) cccTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTc < 1:1958911/68‑1 (MQ=255) ccTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCa < 1:1306150/71‑1 (MQ=255) ccTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCa < 1:2132639/71‑1 (MQ=255) cTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAg < 1:825555/71‑1 (MQ=255) cTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAg > 1:917326/1‑71 (MQ=255) cTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAg < 1:620954/71‑1 (MQ=255) aaCATCGCCTCCAGCGTCACCAGCATCCAGTGTTTCTg < 1:1663325/38‑1 (MQ=255) aaCATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTg < 1:3236500/38‑1 (MQ=255) aaCATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGtt > 1:634628/1‑40 (MQ=255) aaCATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGtt > 1:985012/1‑40 (MQ=255) aaCATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCAc > 1:3447520/1‑43 (MQ=255) aaCATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGaa > 1:645374/1‑61 (MQ=255) aCATCGCCGCCAGCGTCCCCAGCATCCAGTGTTTCTGTTCACCata > 1:789654/1‑46 (MQ=255) aCATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCa > 1:1615056/1‑44 (MQ=255) aCATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGaa > 1:959563/1‑60 (MQ=255) aCATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAg < 1:3682026/68‑1 (MQ=255) aCATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCt > 1:3255222/1‑71 (MQ=255) cATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGt > 1:2809879/1‑37 (MQ=255) aTCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGc < 1:1289206/71‑1 (MQ=255) aTCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGc < 1:2724486/71‑1 (MQ=255) tCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCa < 1:3206601/41‑1 (MQ=255) tCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCa < 1:1761356/41‑1 (MQ=255) tCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGc > 1:3702334/1‑70 (MQ=255) gctgccAGCGTGACGATCATCCAGTGTTTTTGCTCACCAAACTGTTGGCGCTGCATTCCCCAGCCTGCCAt > 1:840797/4‑71 (MQ=255) cgccAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCAt > 1:1375322/1‑69 (MQ=255) cgccAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCAt > 1:3751334/1‑69 (MQ=255) aGCGTGACGATCATCCAGTGTTTTTGCTCACCAAACTGTTGGCGCTg < 1:3275553/47‑1 (MQ=12) gCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCACCCTGCCATCAGCAt < 1:245410/70‑1 (MQ=255) cGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATtg < 1:2839781/71‑1 (MQ=255) gTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGc > 1:2158958/1‑66 (MQ=255) cACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTccc < 1:1958147/49‑1 (MQ=255) | GAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTG > minE/1259749‑1259884 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |