| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,259,838 | T→C | 9.4% | E362E (GAA→GAG) | torZ ← | trimethylamine N‑oxide reductase system III, catalytic subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,259,838 | 0 | T | C | 9.4% | 173.2 / 9.3 | 64 | E362E (GAA→GAG) | torZ | trimethylamine N‑oxide reductase system III, catalytic subunit |
| Reads supporting (aligned to +/- strand): ref base T (28/30); new base C (3/3); total (31/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.70e-01 | |||||||||||
AACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGC > minE/1259771‑1259897 | aaCCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCa < 1:3617065/71‑1 (MQ=255) gccgccCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCa < 1:1517962/70‑1 (MQ=255) cGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCt < 1:3016714/71‑1 (MQ=255) cGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCt > 1:1710474/1‑71 (MQ=255) gtgtACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCa > 1:1072496/1‑62 (MQ=255) tgtgCCGATTTGCCCCAACATTGCTGCCAGCGTGACGATCATCCAGTGTTTTTGCTCACCAAACTGTTGgc < 1:2356310/71‑1 (MQ=255) gtACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCata > 1:2754179/1‑62 (MQ=255) ccAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTg > 1:4101501/1‑62 (MQ=255) ccAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGc > 1:86129/1‑63 (MQ=255) cAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTg < 1:841231/70‑1 (MQ=255) aTCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAAt < 1:3498550/71‑1 (MQ=255) aTCTGCCCTAACATCGCCCCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTCGCGCTGa > 1:2035281/1‑69 (MQ=255) ttGCCCCAACATTGCTGCCAGCGTGACGATCATCCAGTGTTTTTGCTCACCAAACTGTTGGCGCTGCAttg > 1:3651372/2‑70 (MQ=255) cTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGtt > 1:1286498/1‑47 (MQ=255) cTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTc > 1:949744/1‑71 (MQ=255) tGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGgc < 1:1924226/61‑1 (MQ=255) tGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAAtt < 1:3921336/69‑1 (MQ=255) tGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTcc > 1:676311/1‑71 (MQ=255) tGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTcc > 1:489801/1‑71 (MQ=255) tGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTc > 1:2951145/1‑70 (MQ=255) cccTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTc < 1:1958911/68‑1 (MQ=255) ccTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCa < 1:1306150/71‑1 (MQ=255) ccTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCa < 1:2132639/71‑1 (MQ=255) cTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAg < 1:825555/71‑1 (MQ=255) cTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAg > 1:917326/1‑71 (MQ=255) cTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAg < 1:620954/71‑1 (MQ=255) aaCATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGtt > 1:634628/1‑40 (MQ=255) aaCATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGtt > 1:985012/1‑40 (MQ=255) aaCATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCAc > 1:3447520/1‑43 (MQ=255) aaCATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGaa > 1:645374/1‑61 (MQ=255) aCATCGCCGCCAGCGTCCCCAGCATCCAGTGTTTCTGTTCACCata > 1:789654/1‑46 (MQ=255) aCATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCa > 1:1615056/1‑44 (MQ=255) aCATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGaa > 1:959563/1‑60 (MQ=255) aCATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAg < 1:3682026/68‑1 (MQ=255) aCATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCt > 1:3255222/1‑71 (MQ=255) cATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGt > 1:2809879/1‑37 (MQ=255) aTCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGc < 1:2724486/71‑1 (MQ=255) aTCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGc < 1:1289206/71‑1 (MQ=255) tCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCa < 1:1761356/41‑1 (MQ=255) tCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCa < 1:3206601/41‑1 (MQ=255) tCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGc > 1:3702334/1‑70 (MQ=255) gctgccAGCGTGACGATCATCCAGTGTTTTTGCTCACCAAACTGTTGGCGCTGCATTCCCCAGCCTGCCAt > 1:840797/4‑71 (MQ=255) cgccAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCAt > 1:1375322/1‑69 (MQ=255) cgccAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCAt > 1:3751334/1‑69 (MQ=255) aGCGTGACGATCATCCAGTGTTTTTGCTCACCAAACTGTTGGCGCTg < 1:3275553/47‑1 (MQ=12) gCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCACCCTGCCATCAGCAt < 1:245410/70‑1 (MQ=255) cGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATtg < 1:2839781/71‑1 (MQ=255) gTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGc > 1:2158958/1‑66 (MQ=255) cACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTccc < 1:1958147/49‑1 (MQ=255) aCCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGa < 1:1383694/42‑1 (MQ=255) aCCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCg < 1:1953343/71‑1 (MQ=255) gatcATCCAGTGTTTTTGCTCACCAAACTGTTGGCGCTGCATTCCCCAGCCTGCCATCAGCa > 1:3837548/4‑62 (MQ=255) aGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGAtt < 1:1078791/71‑1 (MQ=255) gCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTc > 1:1605191/1‑71 (MQ=255) gCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTc < 1:3837314/71‑1 (MQ=255) tCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGc > 1:213007/1‑45 (MQ=255) tCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGc > 1:3160937/1‑45 (MQ=255) cAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTcccc > 1:3446708/1‑40 (MQ=255) cAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTcccc > 1:961537/1‑40 (MQ=255) cAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTcgccgc < 1:1865392/71‑1 (MQ=255) gtTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCAt > 1:106811/1‑52 (MQ=255) ttttTGCTCACCAAACTGTTGGCGCTGCATTCCCCAGCCTGCCATCa < 1:1316465/47‑1 (MQ=19) tttCTGTTCACGATATTGCTGGCGCTGAATTCCCCAGCCTg < 1:3686287/41‑1 (MQ=255) tttCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTg < 1:1820502/41‑1 (MQ=255) tttCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCa < 1:808004/50‑1 (MQ=255) tttCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTc < 1:1496848/61‑1 (MQ=255) tCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGcc > 1:563965/1‑41 (MQ=255) tGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGc < 1:3151430/38‑1 (MQ=255) | AACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGC > minE/1259771‑1259897 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |