Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,279,173	G→A	9.1%	V318I (GTA→ATA) ‡	yeeJ →	adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,279,173	0	G	A	9.1%	133.0 / 3.8	44	V318I (GTA→ATA) ‡	yeeJ	adhesin
Reads supporting (aligned to +/- strand):  ref base G (18/22);  new base A (2/2);  total (20/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

ACTGACCAACTGGCGCAGCGCACCTGAACTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTA  >  minE/1279103‑1279240                                                                       |                                                                    aCTGACCAACTGGCGCAGCGCACCTGAACTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATg                                                                     <  1:2457052/71‑1 (MQ=255)  cTGACCAACTGGCGCAGCGCACCTGAACTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGt                                                                    <  1:427339/71‑1 (MQ=255)   tGACCAACTGGCGCAGCGCACCTGAACTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTa                                                                   >  1:2794063/1‑71 (MQ=255)     aCCAACTGGCGCAGCGCACCTGAACTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTAcg                                                                 >  1:2180574/1‑71 (MQ=255)     aCCAACTGGCGCAGCGCACCTGAACTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTAcg                                                                 >  1:2175568/1‑71 (MQ=255)       cAACTGGCGCAGCGCACCTGAACTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTAcgcg                                                               <  1:2670775/71‑1 (MQ=255)              cgcagcgcaCCTGAACTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAg                                                        <  1:3811666/71‑1 (MQ=255)              cgcagcgcaCCTGAACTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAg                                                        <  1:1062502/71‑1 (MQ=255)               gcagcgcaCCTGAACTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGc                                                       <  1:2490382/71‑1 (MQ=255)                 agcgcaCCTGAACTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTg                                                     <  1:3966774/71‑1 (MQ=255)                       ccTGAACTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATg                                                                     <  1:2036454/49‑1 (MQ=255)                         tGAACTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCg                                             >  1:1757504/1‑71 (MQ=255)                          gAACTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGc                                                       >  1:1247975/1‑60 (MQ=255)                          gAACTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGc                                            >  1:2566779/1‑71 (MQ=255)                           aaCTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCg                                             <  1:4109024/69‑1 (MQ=255)                             cTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTg                                         <  1:1985323/71‑1 (MQ=255)                                gACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGc                                                       >  1:3662163/1‑54 (MQ=255)                                 aCAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCg                                     <  1:2104505/71‑1 (MQ=255)                                 aCAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCg                                     <  1:2761269/71‑1 (MQ=255)                                   aaCGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTg                                         <  1:956121/65‑1 (MQ=255)                                   aaCGATTATGAAGCACGACCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCt                                          >  1:3196251/1‑64 (MQ=255)                                    aCGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCAc                                  >  1:1625532/1‑71 (MQ=255)                                     ggATTATCAGGAACGCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCagc                                 >  1:3126947/2‑69 (MQ=255)                                      gATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGcc                                           >  1:581064/1‑60 (MQ=255)                                      gATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCt                                >  1:910732/1‑71 (MQ=255)                                      gATTATCAGGAACGCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCt                                <  1:2662854/71‑1 (MQ=255)                                       aTTATCAGGAACGCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGc                                    <  1:1419477/66‑1 (MQ=255)                                              aGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTa                        <  1:2608193/71‑1 (MQ=255)                                              aGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTa                        <  1:2513920/71‑1 (MQ=255)                                              aGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTa                        <  1:485619/71‑1 (MQ=255)                                               gCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTa                        <  1:2702910/70‑1 (MQ=255)                                                        gCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTaaa                      >  1:2985413/1‑63 (MQ=255)                                                         ccAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTa                        <  1:2165892/60‑1 (MQ=255)                                                          cAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTAt             >  1:2391338/1‑70 (MQ=255)                                                          cAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATg            <  1:3805810/71‑1 (MQ=255)                                                            aTGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTa                        <  1:639173/57‑1 (MQ=255)                                                             tGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCagc                                 >  1:3804651/1‑45 (MQ=255)                                                              ggCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTaaa                      >  1:2665002/1‑57 (MQ=255)                                                              ggCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTa              >  1:3931117/1‑65 (MQ=255)                                                               gCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAg       <  1:2032599/71‑1 (MQ=255)                                                               gCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAg       <  1:2589805/71‑1 (MQ=255)                                                                cTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGt      <  1:792457/71‑1 (MQ=255)                                                                    gATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGt                 >  1:4045751/1‑56 (MQ=255)                                                                    gATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGtatta  >  1:3454334/1‑71 (MQ=255)                                                                     aTGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAAc         >  1:539588/1‑63 (MQ=255)                                                                      tGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGta     <  1:1465955/66‑1 (MQ=255)                                                                       |                                                                    ACTGACCAACTGGCGCAGCGCACCTGAACTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTA  >  minE/1279103‑1279240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑