Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,279,175	A→T	9.5%	V318V (GTA→GTT) ‡	yeeJ →	adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,279,175	0	A	T	9.5%	113.5 / 4.8	42	V318V (GTA→GTT) ‡	yeeJ	adhesin
Reads supporting (aligned to +/- strand):  ref base A (17/21);  new base T (2/2);  total (19/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

TGACCAACTGGCGCAGCGCACCTGAACTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTA  >  minE/1279105‑1279240                                                                       |                                                                  tGACCAACTGGCGCAGCGCACCTGAACTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTa                                                                   >  1:2794063/1‑71 (MQ=255)   aCCAACTGGCGCAGCGCACCTGAACTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTAcg                                                                 >  1:2180574/1‑71 (MQ=255)   aCCAACTGGCGCAGCGCACCTGAACTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTAcg                                                                 >  1:2175568/1‑71 (MQ=255)     cAACTGGCGCAGCGCACCTGAACTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTAcgcg                                                               <  1:2670775/71‑1 (MQ=255)            cgcagcgcaCCTGAACTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAg                                                        <  1:1062502/71‑1 (MQ=255)            cgcagcgcaCCTGAACTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAg                                                        <  1:3811666/71‑1 (MQ=255)             gcagcgcaCCTGAACTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGc                                                       <  1:2490382/71‑1 (MQ=255)               agcgcaCCTGAACTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTg                                                     <  1:3966774/71‑1 (MQ=255)                       tGAACTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCg                                             >  1:1757504/1‑71 (MQ=255)                        gAACTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGc                                                       >  1:1247975/1‑60 (MQ=255)                        gAACTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGc                                            >  1:2566779/1‑71 (MQ=255)                         aaCTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCg                                             <  1:4109024/69‑1 (MQ=255)                           cTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTg                                         <  1:1985323/71‑1 (MQ=255)                              gACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGc                                                       >  1:3662163/1‑54 (MQ=255)                               aCAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCg                                     <  1:2104505/71‑1 (MQ=255)                               aCAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCg                                     <  1:2761269/71‑1 (MQ=255)                                 aaCGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTg                                         <  1:956121/65‑1 (MQ=255)                                 aaCGATTATGAAGCACGACCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCt                                          >  1:3196251/1‑64 (MQ=255)                                  aCGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCAc                                  >  1:1625532/1‑71 (MQ=255)                                   ggATTATCAGGAACGCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCagc                                 >  1:3126947/2‑69 (MQ=255)                                    gATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGcc                                           >  1:581064/1‑60 (MQ=255)                                    gATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCt                                >  1:910732/1‑71 (MQ=255)                                    gATTATCAGGAACGCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCt                                <  1:2662854/71‑1 (MQ=255)                                     aTTATCAGGAACGCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGc                                    <  1:1419477/66‑1 (MQ=255)                                            aGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTa                        <  1:2608193/71‑1 (MQ=255)                                            aGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTa                        <  1:485619/71‑1 (MQ=255)                                            aGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTa                        <  1:2513920/71‑1 (MQ=255)                                             gCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTa                        <  1:2702910/70‑1 (MQ=255)                                                      gCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTaaa                      >  1:2985413/1‑63 (MQ=255)                                                       ccAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTa                        <  1:2165892/60‑1 (MQ=255)                                                        cAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTAt             >  1:2391338/1‑70 (MQ=255)                                                        cAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATg            <  1:3805810/71‑1 (MQ=255)                                                          aTGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTa                        <  1:639173/57‑1 (MQ=255)                                                           tGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCagc                                 >  1:3804651/1‑45 (MQ=255)                                                            ggCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTaaa                      >  1:2665002/1‑57 (MQ=255)                                                            ggCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTa              >  1:3931117/1‑65 (MQ=255)                                                             gCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAg       <  1:2589805/71‑1 (MQ=255)                                                             gCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAg       <  1:2032599/71‑1 (MQ=255)                                                              cTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGt      <  1:792457/71‑1 (MQ=255)                                                                  gATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGt                 >  1:4045751/1‑56 (MQ=255)                                                                  gATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGtatta  >  1:3454334/1‑71 (MQ=255)                                                                   aTGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAAc         >  1:539588/1‑63 (MQ=255)                                                                    tGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGta     <  1:1465955/66‑1 (MQ=255)                                                                       aCGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGc                            >  1:2928907/1‑40 (MQ=255)                                                                       aCGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGt                 >  1:1348917/1‑51 (MQ=255)                                                                       |                                                                  TGACCAACTGGCGCAGCGCACCTGAACTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTA  >  minE/1279105‑1279240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑