Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,279,181	A→T	8.3%	A320A (GCA→GCT)	yeeJ →	adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,279,181	0	A	T	8.3%	134.3 / 4.6	48	A320A (GCA→GCT)	yeeJ	adhesin
Reads supporting (aligned to +/- strand):  ref base A (19/25);  new base T (2/2);  total (21/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

CGCAGCGCACCTGAACTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATG  >  minE/1279116‑1279248                                                                  |                                                                    cgcagcgcaCCTGAACTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAg                                                                <  1:3811666/71‑1 (MQ=255) cgcagcgcaCCTGAACTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAg                                                                <  1:1062502/71‑1 (MQ=255)  gcagcgcaCCTGAACTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGc                                                               <  1:2490382/71‑1 (MQ=255)    agcgcaCCTGAACTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTg                                                             <  1:3966774/71‑1 (MQ=255)            tGAACTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCg                                                     >  1:1757504/1‑71 (MQ=255)             gAACTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGc                                                               >  1:1247975/1‑60 (MQ=255)             gAACTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGc                                                    >  1:2566779/1‑71 (MQ=255)              aaCTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCg                                                     <  1:4109024/69‑1 (MQ=255)                cTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTg                                                 <  1:1985323/71‑1 (MQ=255)                   gACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGc                                                               >  1:3662163/1‑54 (MQ=255)                    aCAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCg                                             <  1:2761269/71‑1 (MQ=255)                    aCAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCg                                             <  1:2104505/71‑1 (MQ=255)                      aaCGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTg                                                 <  1:956121/65‑1 (MQ=255)                      aaCGATTATGAAGCACGACCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCt                                                  >  1:3196251/1‑64 (MQ=255)                       aCGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCAc                                          >  1:1625532/1‑71 (MQ=255)                        ggATTATCAGGAACGCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCagc                                         >  1:3126947/2‑69 (MQ=255)                         gATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGcc                                                   >  1:581064/1‑60 (MQ=255)                         gATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCt                                        >  1:910732/1‑71 (MQ=255)                         gATTATCAGGAACGCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCt                                        <  1:2662854/71‑1 (MQ=255)                          aTTATCAGGAACGCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGc                                            <  1:1419477/66‑1 (MQ=255)                                 aGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTa                                <  1:485619/71‑1 (MQ=255)                                 aGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTa                                <  1:2513920/71‑1 (MQ=255)                                 aGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTa                                <  1:2608193/71‑1 (MQ=255)                                  gCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTa                                <  1:2702910/70‑1 (MQ=255)                                           gCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTaaa                              >  1:2985413/1‑63 (MQ=255)                                            ccAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTa                                <  1:2165892/60‑1 (MQ=255)                                             cAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTAt                     >  1:2391338/1‑70 (MQ=255)                                             cAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATg                    <  1:3805810/71‑1 (MQ=255)                                               aTGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTa                                <  1:639173/57‑1 (MQ=255)                                                tGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCagc                                         >  1:3804651/1‑45 (MQ=255)                                                 ggCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTaaa                              >  1:2665002/1‑57 (MQ=255)                                                 ggCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTa                      >  1:3931117/1‑65 (MQ=255)                                                  gCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAg               <  1:2032599/71‑1 (MQ=255)                                                  gCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAg               <  1:2589805/71‑1 (MQ=255)                                                   cTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGt              <  1:792457/71‑1 (MQ=255)                                                       gATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGt                         >  1:4045751/1‑56 (MQ=255)                                                       gATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGtatta          >  1:3454334/1‑71 (MQ=255)                                                        aTGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAAc                 >  1:539588/1‑63 (MQ=255)                                                         tGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGta             <  1:1465955/66‑1 (MQ=255)                                                            aCGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGc                                    >  1:2928907/1‑40 (MQ=255)                                                            aCGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGt                         >  1:1348917/1‑51 (MQ=255)                                                             cgcgCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGa    >  1:438868/1‑71 (MQ=255)                                                              gcgcAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGAt   <  1:3917097/71‑1 (MQ=255)                                                              gcgcAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGAt   >  1:2203185/1‑71 (MQ=255)                                                               cgcAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTc                        <  1:1461261/49‑1 (MQ=255)                                                               cgcAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATg  <  1:3566021/71‑1 (MQ=255)                                                                gcAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATg  <  1:1852795/70‑1 (MQ=255)                                                                gcAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATg  <  1:1401545/70‑1 (MQ=255)                                                                  |                                                                    CGCAGCGCACCTGAACTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATG  >  minE/1279116‑1279248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑