Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 218,097 G→A 8.1% L28L (TTG→TTA yafF → conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE218,0970GA 8.1% 549.8 / 25.3 173L28L (TTG→TTAyafFconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (92/67);  new base A (8/6);  total (100/73)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 173 total aligned reads displayed.

CTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTA  >  minE/218028‑218165
                                                                     |                                                                    
cTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGa                                                                     >  1:3510357/1‑71 (MQ=255)
 tACAAAATAAGAAGAGGAAACCCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGAc                                                                    >  1:3452223/1‑71 (MQ=255)
   cAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACg                                                                   <  1:4025700/70‑1 (MQ=255)
   cAAAATAAGAAGAGGAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACg                                                                   >  1:1544234/1‑70 (MQ=255)
   cAAAATAAGAAGAGGAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACg                                                                   >  1:3335661/1‑70 (MQ=255)
   cAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACg                                                                   >  1:2184564/1‑70 (MQ=255)
    aaaaTAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGa                                                                  <  1:1138848/70‑1 (MQ=255)
        tAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATgag                                                             <  1:843026/71‑1 (MQ=255)
        tAAGAAGAGGAAACGCAGCAGAATAATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAAt                                                                >  1:2261194/1‑68 (MQ=255)
         aagaagAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATgaga                                                            <  1:2361289/71‑1 (MQ=255)
          agaagaGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATgata                                                            <  1:3397028/70‑3 (MQ=255)
             agagGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAgg                                                         <  1:4002204/70‑1 (MQ=255)
             agagGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAgg                                                         <  1:1554768/70‑1 (MQ=255)
               agGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTAt                                                      <  1:1766001/71‑1 (MQ=255)
                ggAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAgg                                                         <  1:2449708/67‑1 (MQ=255)
                ggAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTAtt                                                     >  1:2033332/1‑71 (MQ=255)
                  aaaTGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTc                                                    <  1:2087874/70‑1 (MQ=255)
                   aaCGCAGAAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAgg                                                         <  1:335349/64‑1 (MQ=255)
                    aCGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGaa                                                           >  1:2303880/1‑61 (MQ=255)
                      gcagcaGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGa                                                                  <  1:1337199/52‑1 (MQ=255)
                        agcagAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAg                                             <  1:3959747/71‑1 (MQ=255)
                        agcagAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCa                                              <  1:3272177/70‑1 (MQ=255)
                            gAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGtt                                         <  1:3020988/71‑1 (MQ=255)
                             aaTTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTc                                                    <  1:3529575/59‑1 (MQ=255)
                               ttattTTCAGGGATACGGCACATTGCTATTAATATTTTGACGaa                                                                 >  1:561752/1‑44 (MQ=255)
                               ttattTTCAGGGATACGGCACATTGCTATTAATATTTTGACGaa                                                                 >  1:1262472/1‑44 (MQ=255)
                               ttattTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTa                                                       >  1:3325708/1‑54 (MQ=255)
                               ttattTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGt                                          >  1:2042152/1‑67 (MQ=255)
                               ttattTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTa                                                       >  1:428243/1‑54 (MQ=255)
                               ttattTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTc                                                    <  1:2369212/57‑1 (MQ=255)
                                tattTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGa                                     >  1:3755574/1‑71 (MQ=255)
                                 attTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGCTAAGa                                     <  1:1009832/70‑1 (MQ=255)
                                  ttttCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTaa                                       <  1:1928564/67‑1 (MQ=255)
                                   tttCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAgg                                                         >  1:14288/1‑48 (MQ=255)
                                    ttCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGt                                  >  1:896646/1‑70 (MQ=255)
                                     tCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTa                                 <  1:1200782/70‑1 (MQ=255)
                                      cAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGtt                                         <  1:1082554/61‑1 (MQ=255)
                                      cAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTaa                                <  1:1493411/70‑1 (MQ=255)
                                       aGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAg                                                          <  1:4058393/43‑1 (MQ=255)
                                         ggATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTc                                                    <  1:2658287/47‑1 (MQ=255)
                                         ggATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTc                                                    <  1:762922/47‑1 (MQ=255)
                                          gATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGc                           >  1:32025/1‑71 (MQ=255)
                                           aTACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTc                                                    >  1:3922228/1‑45 (MQ=255)
                                             aCGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGa                         >  1:168842/1‑70 (MQ=255)
                                              cGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTaa                                       >  1:894209/1‑55 (MQ=255)
                                              cGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTa                                                       >  1:116248/1‑39 (MQ=255)
                                              cGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTaa                                       >  1:2223755/1‑55 (MQ=255)
                                              cGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTaa                                >  1:1518953/1‑62 (MQ=255)
                                              cGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGaa                        <  1:1139031/70‑1 (MQ=255)
                                              cGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGaa                        >  1:3708275/1‑70 (MQ=255)
                                              cGGCACATTGCTATTAATATTTCGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGaa                        >  1:2273019/1‑70 (MQ=255)
                                                  acaTTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGa                                     >  1:2678303/1‑53 (MQ=255)
                                                  acaTTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAag                     >  1:471657/1‑69 (MQ=255)
                                                   caTCGCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGa                         >  1:2394826/1‑64 (MQ=255)
                                                   caTCGCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAagcag                  <  1:1212147/71‑1 (MQ=255)
                                                    aTTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAagcagc                 >  1:974694/1‑71 (MQ=255)
                                                    aTTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAagcagc                 >  1:1240525/1‑71 (MQ=255)
                                                    aTTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGtt                                         <  1:393297/47‑1 (MQ=255)
                                                    aTTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAagcagc                 <  1:2710014/71‑1 (MQ=255)
                                                    aTTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAagcagc                 <  1:2201089/71‑1 (MQ=255)
                                                     ttGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAagcagc                 >  1:3886734/1‑70 (MQ=255)
                                                     ttGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAg                                      >  1:237797/1‑49 (MQ=255)
                                                     ttGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGa                              >  1:193355/1‑57 (MQ=255)
                                                     ttGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAagcagc                 >  1:181336/1‑70 (MQ=255)
                                                     tcgCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAagcagc                 >  1:1843493/3‑70 (MQ=255)
                                                      tGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCa               >  1:1233439/1‑71 (MQ=255)
                                                       gCTATTAATATTTTGACGAATGAGAAGGTATTCaa                                                  >  1:1695974/1‑35 (MQ=255)
                                                       gCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCAt              >  1:3892860/1‑71 (MQ=255)
                                                       gCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCAt              >  1:1811614/1‑71 (MQ=255)
                                                           ttAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTaa                                       <  1:2563745/42‑1 (MQ=255)
                                                             aaTATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTaa                                       >  1:3218936/1‑40 (MQ=255)
                                                             aaTATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGa           >  1:1397272/1‑68 (MQ=255)
                                                             aaTATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAag                     >  1:2727963/1‑58 (MQ=255)
                                                             aaTATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGa           >  1:2117542/1‑68 (MQ=255)
                                                             aaTATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGa                              >  1:2789989/1‑49 (MQ=255)
                                                             aaTATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGa           >  1:2444284/1‑68 (MQ=255)
                                                              atatTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAg        >  1:3181590/1‑70 (MQ=255)
                                                              atatTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAg        <  1:2291658/70‑1 (MQ=255)
                                                               tatTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGaaaa                      >  1:104092/1‑55 (MQ=255)
                                                               tatTTTGACGAATGAGAAGGTATTCAAGGCAGGGtt                                         <  1:3504522/36‑1 (MQ=255)
                                                               tatTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAATATGCGAAAAGCAGCCATGGACAGaa      <  1:647353/71‑1 (MQ=255)
                                                               tatTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGcc                >  1:2048841/1‑61 (MQ=255)
                                                               tatTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGa       <  1:145670/70‑1 (MQ=255)
                                                                atTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGaaaa                      >  1:1217459/1‑54 (MQ=255)
                                                                atTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGAc          >  1:3642772/1‑66 (MQ=255)
                                                                 ttttGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGa       <  1:3254819/68‑1 (MQ=255)
                                                                 ttttGACGAATGAGAATGTATTCAAGGCAGGGTTAAGACGt                                  <  1:3294372/41‑1 (MQ=255)
                                                                 ttttGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGAc                                    >  1:775226/1‑39 (MQ=255)
                                                                 ttttGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAAc    >  1:1898489/1‑71 (MQ=255)
                                                                 ttttGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAAc    >  1:789687/1‑71 (MQ=255)
                                                                 ttttGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAAc    <  1:3823550/71‑1 (MQ=255)
                                                                  tttGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATg             <  1:1946209/61‑1 (MQ=255)
                                                                  tttGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGa       <  1:1430416/67‑1 (MQ=255)
                                                                  tttAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATg                            >  1:959876/1‑46 (MQ=255)
                                                                   ttGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAagcag                  >  1:51470/1‑55 (MQ=255)
                                                                   ttGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTaa                                >  1:2946894/1‑41 (MQ=255)
                                                                   ttGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAagcag                  >  1:68080/1‑55 (MQ=255)
                                                                   ttGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAagcag                  >  1:899948/1‑55 (MQ=255)
                                                                    tGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGa           >  1:1444284/1‑61 (MQ=255)
                                                                    tGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTa  <  1:291078/70‑1 (MQ=255)
                                                                     |                                                                    
CTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTA  >  minE/218028‑218165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: