Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,279,185	A→G	8.2%	S322G (AGC→GGC)	yeeJ →	adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,279,185	0	A	G	8.2%	137.0 / 4.1	49	S322G (AGC→GGC)	yeeJ	adhesin
Reads supporting (aligned to +/- strand):  ref base A (19/26);  new base G (2/2);  total (21/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.93e-01

CGCAGCGCACCTGAACTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGG  >  minE/1279116‑1279254                                                                      |                                                                      cgcagcgcaCCTGAACTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAg                                                                      <  1:1062502/71‑1 (MQ=255) cgcagcgcaCCTGAACTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAg                                                                      <  1:3811666/71‑1 (MQ=255)  gcagcgcaCCTGAACTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGc                                                                     <  1:2490382/71‑1 (MQ=255)    agcgcaCCTGAACTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTg                                                                   <  1:3966774/71‑1 (MQ=255)            tGAACTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCg                                                           >  1:1757504/1‑71 (MQ=255)             gAACTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGc                                                                     >  1:1247975/1‑60 (MQ=255)             gAACTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGc                                                          >  1:2566779/1‑71 (MQ=255)              aaCTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCg                                                           <  1:4109024/69‑1 (MQ=255)                cTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTg                                                       <  1:1985323/71‑1 (MQ=255)                   gACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGc                                                                     >  1:3662163/1‑54 (MQ=255)                    aCAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCg                                                   <  1:2761269/71‑1 (MQ=255)                    aCAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCg                                                   <  1:2104505/71‑1 (MQ=255)                      aaCGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTg                                                       <  1:956121/65‑1 (MQ=255)                      aaCGATTATGAAGCACGACCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCt                                                        >  1:3196251/1‑64 (MQ=255)                       aCGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCAc                                                >  1:1625532/1‑71 (MQ=255)                        ggATTATCAGGAACGCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCagc                                               >  1:3126947/2‑69 (MQ=255)                         gATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGcc                                                         >  1:581064/1‑60 (MQ=255)                         gATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCt                                              >  1:910732/1‑71 (MQ=255)                         gATTATCAGGAACGCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCt                                              <  1:2662854/71‑1 (MQ=255)                          aTTATCAGGAACGCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGc                                                  <  1:1419477/66‑1 (MQ=255)                                 aGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTa                                      <  1:485619/71‑1 (MQ=255)                                 aGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTa                                      <  1:2513920/71‑1 (MQ=255)                                 aGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTa                                      <  1:2608193/71‑1 (MQ=255)                                  gCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTa                                      <  1:2702910/70‑1 (MQ=255)                                           gCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTaaa                                    >  1:2985413/1‑63 (MQ=255)                                            ccAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTa                                      <  1:2165892/60‑1 (MQ=255)                                             cAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTAt                           >  1:2391338/1‑70 (MQ=255)                                             cAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATg                          <  1:3805810/71‑1 (MQ=255)                                               aTGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTa                                      <  1:639173/57‑1 (MQ=255)                                                tGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCagc                                               >  1:3804651/1‑45 (MQ=255)                                                 ggCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTaaa                                    >  1:2665002/1‑57 (MQ=255)                                                 ggCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTa                            >  1:3931117/1‑65 (MQ=255)                                                  gCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAg                     <  1:2032599/71‑1 (MQ=255)                                                  gCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAg                     <  1:2589805/71‑1 (MQ=255)                                                   cTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGt                    <  1:792457/71‑1 (MQ=255)                                                       gATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGt                               >  1:4045751/1‑56 (MQ=255)                                                       gATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGtatta                >  1:3454334/1‑71 (MQ=255)                                                        aTGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAAc                       >  1:539588/1‑63 (MQ=255)                                                         tGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGta                   <  1:1465955/66‑1 (MQ=255)                                                            aCGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGc                                          >  1:2928907/1‑40 (MQ=255)                                                            aCGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGt                               >  1:1348917/1‑51 (MQ=255)                                                             cgcgCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGa          >  1:438868/1‑71 (MQ=255)                                                              gcgcAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGAt         <  1:3917097/71‑1 (MQ=255)                                                              gcgcAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGAt         >  1:2203185/1‑71 (MQ=255)                                                               cgcAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTc                              <  1:1461261/49‑1 (MQ=255)                                                               cgcAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATg        <  1:3566021/71‑1 (MQ=255)                                                                gcAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATg        <  1:1852795/70‑1 (MQ=255)                                                                gcAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATg        <  1:1401545/70‑1 (MQ=255)                                                                   gAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAAc                       <  1:1865654/52‑1 (MQ=255)                                                                     aaGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGc            >  1:1910476/1‑61 (MQ=255)                                                                     aaGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTgg  >  1:2354644/1‑71 (MQ=255)                                                                     aaGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTgg  <  1:1878218/71‑1 (MQ=255)                                                                      |                                                                      CGCAGCGCACCTGAACTGGACAACGATTATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGG  >  minE/1279116‑1279254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑