| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,289,208 | C→T | 17.8% | G279G (GGC→GGT) | amn → | AMP nucleosidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,289,208 | 0 | C | T | 17.8% | 133.2 / 20.7 | 56 | G279G (GGC→GGT) | amn | AMP nucleosidase |
| Reads supporting (aligned to +/- strand): ref base C (19/27); new base T (6/4); total (25/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01 | |||||||||||
AAAAACATCAGATGCCAGCATGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGCCCGGATGTCTGGTTGATGA > minE/1289138‑1289278 | aaaaaCATCAGATGCCAGCATGGTATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGc > 1:182990/1‑71 (MQ=255)aaaaaCATCAGATGCCAGCATGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGc > 1:447110/1‑71 (MQ=255) aaCATCAGATGCCAGCATGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTg > 1:97760/1‑71 (MQ=255) aaCATCAGATGCCAGCATGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTg > 1:2424822/1‑71 (MQ=255) aGATGCCAGCATGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCg < 1:3236950/71‑1 (MQ=255) tGCCAGCATGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGTGTGGGACCGTCa < 1:1350749/71‑1 (MQ=255) tGCCAGCATGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGTGTGGGACCGTCa < 1:186888/71‑1 (MQ=255) ggCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCaaa > 1:2863304/1‑64 (MQ=255) ggCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTaa < 1:3464336/70‑1 (MQ=255) aTTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCaaa > 1:1204020/1‑61 (MQ=255) aTTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAAcc < 1:2545938/71‑1 (MQ=255) aTTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAAcc < 1:1610222/71‑1 (MQ=255) ttAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGtgtg < 1:3470915/48‑1 (MQ=255) tAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTg > 1:850124/1‑47 (MQ=255) tAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCAt > 1:2241740/1‑70 (MQ=255) tAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATc > 1:1006269/1‑71 (MQ=255) aTTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGTGTGGGACCGTCAAATGCTAAAACCATCTg < 1:867634/71‑1 (MQ=255) aTTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGAc < 1:1556312/49‑1 (MQ=255) aTTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGAc < 1:3945695/49‑1 (MQ=255) aTTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGAc < 1:598075/49‑1 (MQ=255) aCCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGTGTGGGACCGTCAAATGCTAAAACCATCTGCGa > 1:1186662/1‑71 (MQ=255) aCCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTc > 1:1406270/1‑50 (MQ=255) aCCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCg < 1:3411921/70‑1 (MQ=255) cgccgATGGTCAGGGTATTACTCTGGTGAATATTGGTGTGGGACCGTCAAATGCTAAAACCATCTGCGatc < 1:3961532/71‑1 (MQ=255) cgccgATGGTCAGGGTATTACTCTGGTGAATATTGGCGTggg < 1:125812/42‑1 (MQ=255) ccgATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGatcat < 1:471331/71‑1 (MQ=255) gATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCt < 1:2924282/71‑1 (MQ=255) aTGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCt < 1:332225/70‑1 (MQ=255) tCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAg < 1:3793893/71‑1 (MQ=255) cAGGGTATTACTCTGGTGAATATTGGTGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGt > 1:2498164/1‑71 (MQ=255) aGGGTATTACTCTGGTGAATATTGGTGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTg > 1:686818/1‑71 (MQ=255) gggTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCt < 1:2972279/63‑1 (MQ=255) gggTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGc > 1:2798200/1‑71 (MQ=255) ggTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGc > 1:2303443/1‑70 (MQ=255) ttACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGc < 1:749184/50‑1 (MQ=255) ttACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTgg < 1:1322142/60‑1 (MQ=255) ttACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGc < 1:1116043/66‑1 (MQ=255) aCTCTGGTGAATATTGGTGTGGGACCGTCAAATGCTAAAACCATCTGCGatcat > 1:905057/1‑54 (MQ=255) aCTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCt > 1:2018615/1‑56 (MQ=255) aCTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGccc > 1:2397635/1‑71 (MQ=255) aCTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGccc > 1:2058775/1‑71 (MQ=255) aCTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCAACTGCGATCATCTGGCAGTGCTACGccc > 1:2095537/1‑71 (MQ=255) ctctGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGCCCg > 1:1020816/1‑71 (MQ=255) tctGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCg < 1:1028300/47‑1 (MQ=255) tctGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGatc < 1:2918721/50‑1 (MQ=255) tctGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGatc < 1:191806/50‑1 (MQ=255) tctGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGatc < 1:344665/50‑1 (MQ=255) ctGGTGAATATTGGCGTGGGACCGTCAAATGCTaaaa > 1:2774925/1‑37 (MQ=255) ctGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCg < 1:2444818/46‑1 (MQ=255) tGGTGAATATTGGTGTGGGACCGTCAAATGCTAAAACCATCTGCGat > 1:1961115/1‑47 (MQ=255) tGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGCCCg < 1:4105022/68‑1 (MQ=255) ggTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGc < 1:3867007/59‑1 (MQ=255) gTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAg < 1:3482283/55‑1 (MQ=255) tGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTa > 1:255736/1‑59 (MQ=255) gAATATTGGTGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGCCCGGATGTCt > 1:420944/1‑71 (MQ=255) gAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGCCCGGATGTCt > 1:3354228/1‑71 (MQ=255) aaTATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGCCCg < 1:2839997/63‑1 (MQ=255) tatTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGt > 1:3912141/1‑51 (MQ=255) ttGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGCCCGGATGTCTGGTtg < 1:1338004/71‑1 (MQ=255) cGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGCCCGGATGTCTGGTtgatga < 1:3894311/71‑1 (MQ=255) | AAAAACATCAGATGCCAGCATGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGCCCGGATGTCTGGTTGATGA > minE/1289138‑1289278 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |