| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,309,899 | G→A | 19.0% | R31R (CGC→CGT) | yefM ← | antitoxin of the YoeB‑YefM toxin‑antitoxin system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,309,899 | 0 | G | A | 19.0% | 144.5 / 26.3 | 63 | R31R (CGC→CGT) | yefM | antitoxin of the YoeB‑YefM toxin‑antitoxin system |
| Reads supporting (aligned to +/- strand): ref base G (29/22); new base A (7/5); total (36/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01 | |||||||||||
AAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCT > minE/1309835‑1309968 | aaGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAg > 1:841505/1‑69 (MQ=255) aGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGtaa < 1:51469/71‑1 (MQ=255) aGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGtaa > 1:2934828/1‑71 (MQ=255) aGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGta < 1:392709/70‑1 (MQ=255) ccGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGtaata < 1:2364932/71‑1 (MQ=255) tctcTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAgg < 1:3752674/71‑1 (MQ=255) ctctTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGa > 1:1427803/1‑71 (MQ=255) tctTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGAt > 1:816267/1‑71 (MQ=255) tctTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGAt > 1:416646/1‑71 (MQ=255) actTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGAt < 1:3079396/70‑1 (MQ=255) ttCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAgg < 1:1265221/67‑1 (MQ=255) ttCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGa < 1:3804850/68‑1 (MQ=255) ttCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGAt < 1:3848843/69‑1 (MQ=255) ttCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGAt < 1:2265764/69‑1 (MQ=255) gCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCACCATTCTGGCGAGTAATAAGGATCGGGGCa > 1:3920795/1‑71 (MQ=255) cGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCAt < 1:2464254/71‑1 (MQ=255) gTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGAt > 1:2962877/1‑57 (MQ=255) tATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTc < 1:823806/71‑1 (MQ=255) tATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTc < 1:1725498/71‑1 (MQ=255) ttcttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCaa > 1:3182559/1‑71 (MQ=255) cttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGtaata < 1:4115021/47‑1 (MQ=255) ttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCggg > 1:238495/1‑55 (MQ=255) ttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGAt > 1:846383/1‑51 (MQ=255) ttcGAGGGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCg > 1:7725/1‑53 (MQ=255) gAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTc < 1:3897882/63‑1 (MQ=255) gTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCttt > 1:1964067/1‑70 (MQ=255) aCATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTtcat < 1:1933302/70‑1 (MQ=255) cATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTtcatc < 1:206156/70‑1 (MQ=255) aTCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGAGCGGGGCATGATCTTCAACGGCTTtcatc < 1:44153/69‑1 (MQ=255) tCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAgg < 1:1290463/38‑1 (MQ=255) tCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCgg < 1:3405641/43‑1 (MQ=255) tCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATg < 1:2769034/49‑1 (MQ=255) tCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGAt < 1:448531/51‑1 (MQ=255) tCAGAACACAAGCCTCTCCATTCTGACGAGtaata < 1:3161205/35‑1 (MQ=255) aGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATc > 1:286869/1‑50 (MQ=255) aGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCt > 1:3834369/1‑51 (MQ=255) aGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCaa > 1:2899844/1‑55 (MQ=255) aGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCaa > 1:811892/1‑55 (MQ=255) aGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTcatcat > 1:3301298/1‑68 (MQ=255) aGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCAttgt > 1:3952156/1‑71 (MQ=255) aGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCAttgt > 1:93541/1‑71 (MQ=255) aGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCAttg < 1:940401/70‑1 (MQ=255) aaCACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCAttgttg > 1:3457566/1‑71 (MQ=255) caAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGa > 1:1897116/1‑71 (MQ=255) aGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGAc > 1:1339638/1‑70 (MQ=255) aGCCACTCCATTCTGGCGAGTAATAAGGATCGGGGCATGa > 1:1962070/1‑40 (MQ=255) ctctCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACaaa > 1:850977/1‑70 (MQ=255) ctcCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTc > 1:3345463/1‑71 (MQ=255) tcCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACaaa > 1:2216262/1‑67 (MQ=255) ccATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACaaa > 1:410939/1‑66 (MQ=255) ccATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCt > 1:2660372/1‑70 (MQ=255) cATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCt < 1:1344552/69‑1 (MQ=255) cATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGa > 1:2231723/1‑71 (MQ=255) cATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCt < 1:3312259/69‑1 (MQ=255) cATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTg < 1:149327/70‑1 (MQ=255) cATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGa > 1:3253101/1‑71 (MQ=255) tCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTtc > 1:2308623/1‑44 (MQ=255) tCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTtc > 1:2308210/1‑44 (MQ=255) tCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTtc > 1:1026285/1‑44 (MQ=255) cTGGCGAGTAATAAGGATCGGGGCATGATCTTCaa > 1:1342691/1‑35 (MQ=255) cTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGAcgcg > 1:1597187/1‑71 (MQ=255) cTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGAcgcg > 1:2644187/1‑71 (MQ=255) cTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGAcgc < 1:702546/70‑1 (MQ=255) ggCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCt > 1:1633446/1‑71 (MQ=255) gCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAAt < 1:3074019/59‑1 (MQ=255) | AAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCT > minE/1309835‑1309968 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |