| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,314,255 | T→C | 18.4% | H170H (CAT→CAC) | hisB → | fused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,314,255 | 0 | T | C | 18.4% | 282.3 / 57.7 | 125 | H170H (CAT→CAC) | hisB | fused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase |
| Reads supporting (aligned to +/- strand): ref base T (47/55); new base C (14/9); total (61/64) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
Only 100 of 126 total aligned reads displayed.
TACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAA > minE/1314187‑1314323 | tACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCAt > 1:2750104/1‑69 (MQ=255)tACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATg > 1:781509/1‑70 (MQ=255)tACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATg > 1:136050/1‑70 (MQ=255)tACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATg > 1:2781301/1‑70 (MQ=255)tACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATg > 1:1745829/1‑70 (MQ=255) cGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTa < 1:3034328/70‑1 (MQ=255) cGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGt < 1:629060/69‑1 (MQ=255) ccGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGc < 1:1628333/71‑1 (MQ=255) gcgAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGt < 1:1917839/71‑1 (MQ=255) aaaCCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTaa < 1:1936855/70‑1 (MQ=255) aaaCCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTaa < 1:2910389/70‑1 (MQ=255) aCCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAAt > 1:3772008/1‑69 (MQ=255) cccTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTaa > 1:4130530/1‑67 (MQ=255) ccTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCa < 1:825592/71‑1 (MQ=255) cTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGt < 1:2877820/56‑1 (MQ=255) cTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGt < 1:2304491/56‑1 (MQ=255) gAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTaa > 1:2338797/1‑63 (MQ=255) aCTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAag < 1:757001/69‑1 (MQ=255) cTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAgaga > 1:3933083/1‑71 (MQ=255) cTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGAGTAATACCAAAgaga > 1:2493918/1‑71 (MQ=255) ggCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACg > 1:3823304/1‑71 (MQ=255) gCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGCCg > 1:3711809/1‑70 (MQ=255) ccAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCa < 1:2115196/71‑1 (MQ=255) cAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACg > 1:2823416/1‑68 (MQ=255) aaTGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTACTACCAAAGAGACg > 1:2598809/1‑67 (MQ=255) aTGATTGGCGAGCAACTCACCAGACTTGACCGTTACGCTCATGTAGTGCGTaa > 1:92977/1‑53 (MQ=255) aTGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAt < 1:2897383/71‑1 (MQ=255) tGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATg > 1:4137841/1‑42 (MQ=255) tGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTa > 1:2069914/1‑44 (MQ=255) tGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAg > 1:3985364/1‑45 (MQ=255) tGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAg > 1:2833321/1‑45 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTaa > 1:1024984/1‑50 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTg < 1:2355388/70‑1 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTg < 1:3927231/70‑1 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTg < 1:3857566/70‑1 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGa > 1:1687928/1‑71 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGa > 1:1275704/1‑71 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGt < 1:1695105/48‑1 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTaa > 1:785557/1‑50 (MQ=255) ttGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTg < 1:1182371/69‑1 (MQ=255) ttGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGAc > 1:3451305/1‑71 (MQ=255) ttGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGAc > 1:897130/1‑71 (MQ=255) tGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGAc > 1:3183148/1‑70 (MQ=255) tGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGAc > 1:2162095/1‑70 (MQ=255) ggCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTg < 1:487166/67‑1 (MQ=255) ggCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTg < 1:113065/67‑1 (MQ=255) ggCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTg < 1:3658369/67‑1 (MQ=255) ggCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTg < 1:355644/67‑1 (MQ=255) ggCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGt > 1:145848/1‑71 (MQ=255) gCGAGCAACTCACCAGACGTGACCGTTACGCTCATg < 1:1426739/36‑1 (MQ=255) cGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATa > 1:2018943/1‑47 (MQ=255) cGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATa > 1:2611087/1‑47 (MQ=255) cGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTc < 1:2224189/71‑1 (MQ=255) aGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAg < 1:4066028/71‑1 (MQ=255) aGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAg < 1:3776668/71‑1 (MQ=255) aGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAg < 1:1951248/71‑1 (MQ=255) gCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCaaa > 1:2827190/1‑49 (MQ=255) gCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGAc > 1:452290/1‑64 (MQ=255) gCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAgg < 1:2772566/71‑1 (MQ=255) gCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAgg < 1:3250726/71‑1 (MQ=255) gCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAgg > 1:2699141/1‑71 (MQ=255) aaCTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGgtg < 1:2210775/71‑1 (MQ=255) aCTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGgtgt < 1:598174/71‑1 (MQ=255) aCTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGgtgt < 1:2503197/71‑1 (MQ=255) cacACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGtgtg > 1:1998222/3‑71 (MQ=255) cTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAgaga > 1:2461868/1‑49 (MQ=255) cTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAgaga < 1:4007307/49‑1 (MQ=255) cTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCa < 1:2480623/53‑1 (MQ=255) cTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGtgtg > 1:2566771/1‑71 (MQ=255) cTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGtgtg < 1:1496360/71‑1 (MQ=255) cTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGtgtg > 1:3923354/1‑71 (MQ=255) cACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACg > 1:1584824/1‑49 (MQ=255) cACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGc > 1:325090/1‑71 (MQ=255) cACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGc > 1:1292661/1‑71 (MQ=255) cACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGc > 1:3216261/1‑71 (MQ=255) cACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGc > 1:320075/1‑71 (MQ=255) aCCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTgg < 1:3153055/69‑1 (MQ=255) aCCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTgg < 1:4143712/69‑1 (MQ=255) aCCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCt > 1:3767103/1‑71 (MQ=255) aGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGAc > 1:4008615/1‑54 (MQ=255) aGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGa < 1:3900635/71‑1 (MQ=255) aGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGa < 1:1834016/71‑1 (MQ=255) aGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGa > 1:3643766/1‑71 (MQ=255) aCGTGACCGTTACGCTCATGTAGTGCGTAATACCaaa < 1:2122438/37‑1 (MQ=255) aCGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATc < 1:2712540/71‑1 (MQ=255) cGTGACCGTTACGCTCATGTAGTGCGTAATACCAAag < 1:878925/37‑1 (MQ=255) cGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATc > 1:399600/1‑70 (MQ=255) cGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATc < 1:3697173/70‑1 (MQ=255) aCCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGTCTGGATCGTGaa < 1:1125249/71‑1 (MQ=255) gTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACg < 1:3055327/45‑1 (MQ=255) gTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAgg < 1:306051/51‑1 (MQ=255) gTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAgg < 1:3175574/51‑1 (MQ=255) aCGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTgg > 1:2095505/1‑70 (MQ=255) gCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGgcag > 1:188870/1‑71 (MQ=255) cTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGgcagc < 1:1802161/71‑1 (MQ=255) cTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGgcagc < 1:1439660/71‑1 (MQ=255) cTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGgcagc < 1:3316506/71‑1 (MQ=255) tCATGTAGTTCGTAATACCAAAGAGACGCAGATTGACGtt < 1:1267507/40‑1 (MQ=255) aTGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCaa < 1:2987186/70‑1 (MQ=255) aTGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCaa < 1:3378783/70‑1 (MQ=255) | TACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAA > minE/1314187‑1314323 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |