| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,334,615 | G→A | 53.1% | L72L (CTG→TTG) | nudD ← | GDP‑mannose mannosyl hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,334,615 | 0 | G | A | 53.1% | ‑0.9 / 67.5 | 49 | L72L (CTG→TTG) | nudD | GDP‑mannose mannosyl hydrolase |
| Reads supporting (aligned to +/- strand): ref base G (12/11); new base A (14/12); total (26/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.63e-01 | |||||||||||
CCAGAGAAGTTATCGTCATAAAAGTGCTGCCAGACACCGTAAAACTGGCCTGCTGTTATCGGCAAACGCAGCCCCAGTTCCGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTC > minE/1334545‑1334683 | ccAGAGAAGTTATCGTCATAAAAGTGCTGCCAGACACCGTAAAACTGGCCTGCTGTTATCGGCAAACGCAg > 1:3005836/1‑71 (MQ=255) agagAATTTATCGTCATAAAAGTGCTGCCAGACACCGTAAAACTGGCCTGCTGTTATCGGCAAACGCAAcc < 1:1246343/71‑1 (MQ=255) ttATCGTCATAAAAGTGCTGCCAGACACCGTAAAACTGGCCTGCTGTTATCGGCAAACGCAAccc < 1:3206323/65‑1 (MQ=255) aTCGTCATAAAAGTGCTGCCAGACACCGTAAAACTGGCCTGCTGTTATCGGCAAACGCAGCCCCAGTTCCg > 1:1495666/1‑71 (MQ=255) gTCATAAAAGTGCTGCCAGACACCGTAAAACTGGCCTGCTGTTATCGGCAAACGCAGCCCCAGTTCCGCCa > 1:376374/1‑71 (MQ=255) gTCATAAAAGTGCTGCCAGACACCGTAAAACTGGCCTGCTGTTATCGGCAAACGCAACCCCAGTTCCGcc < 1:3224984/70‑1 (MQ=255) aGTGCTGCCAGACACCGTAAAACTGGCCTGCTGTTATCGGCAAACGCAGCCCCAGTTCCGCCATCGTCAGc > 1:180522/1‑71 (MQ=255) tctgACACCGTAAAACTGGCCTGCTGTTATCGGCAAACGCAACCCCAGTTCCGCCATCGTCAGCCGCTCa < 1:3761212/67‑1 (MQ=255) ccAGACACCGTAAAACTGGCCTGCTGTTATCGGCAAACGCAGCCCCa > 1:2342348/1‑47 (MQ=255) ccAGACACCGTAAAACTGGCCTGCTGTTATCGGCAAACGCAACCCCAGTTCCGCCATCGTCAGCCGCTCa < 1:1345481/70‑1 (MQ=255) ccAGACACCGTAAAACTGGCCTGCTGTTATCGGCAAACGCAACCCCAGTTCCGCCATCGTCAGCCGCTCa < 1:3455260/70‑1 (MQ=255) ccAGACACCGTAAAACTGGCCTGCTGTTATCGGCAAACGCAACCCCAGTTCCGCCATCGTCAGCCGCTCa < 1:1888888/70‑1 (MQ=255) aGACACCGTAAAACTGGCCTGCTGTTATCGGCAAACGCAACCCCAGTTCCGCCATCGTCAGCCGCTCAAAt < 1:2219515/71‑1 (MQ=255) acacCGTAAAACTGGCCTGCTGTTATCGGCAAACGCAGCCCCAGTTCCGCCATCGTCAGCCGCTCAAATGc < 1:604847/71‑1 (MQ=255) acacCGTAAAACTGGCCTGCTGTTATCGGCAAACGCAACCCCAGTTCCGCCATCGTCAGCCGCTCa < 1:1729528/66‑1 (MQ=255) acacCGTAAAACTGGCCTGCTGTTATCGGCAAACGCAACCCCAGTTCCGCCATCGTCAGCCGCTCAAATg > 1:1940163/1‑70 (MQ=255) ccGTAAAACTGGCCTGCTGTTATCGGCAAACGCAACCCCAGTTCCGCCATc < 1:3670225/51‑1 (MQ=255) cGTAAAACTGGCCTGCTGTTATCGGCAAACGCAGCCCCAGTTCCGCCATCGTCAGCCGCTCAAATGCGGCt < 1:1317938/71‑1 (MQ=255) cGTAAAACTGGCCTGCTGTTATCGGCAAACGCAGCCCCAGTTCCGCCATCGTCAGCCGCTCAAATGCGGCt < 1:3352234/71‑1 (MQ=255) gTAAAACTGGCCTGCTGTTATCGGCAAACGCAGCCCCAGTTCCGCCAt > 1:3807535/1‑48 (MQ=255) gTAAAACTGGCCTGCTGTTATCGGCAAACGCAGCCCCAGTTCCGCCATCGTCAGCCGc < 1:2912221/58‑1 (MQ=255) gTAAAACTGGCCTGCTGTTATCGGCAAACGCAACCCCAGTTCCGCCATCGTCAGCCGCTCAAATGCGGCtt > 1:56535/1‑71 (MQ=255) tAAAACTGGCCTGCTGTTATCGGCAAACGCAGccc > 1:501594/1‑35 (MQ=255) tAAAACTGGCCTGCTGTTATCGGCAAACGCAGCCCCAGTTCCGCCATCGTCAGCCGCTCAAATg > 1:3926491/1‑64 (MQ=255) tAAAACTGGCCTGCTGTTATCGGCAAACGCAGCCCCAGTTCCGCCATCGTCAGCCGCTCAAATg > 1:2860575/1‑64 (MQ=255) aaaaCTGGCCTGCTGTTATCGGCAAACGCAACCCCAGTTCCGCCATCGTCAGCCGCTCAAATGCGGCTTc > 1:1647896/1‑70 (MQ=255) aaaCTGGCCTGCTGTTATCGGCAAACGCAGCCCCAGTTCCGCCATCGTCAGCCGCTCAAATGCGGCTTc > 1:1962450/1‑69 (MQ=255) aaaCTGGCCTGCTGTTATCGGCAAACGCAGCCCCAGTTCAGCCATCGTCAGCCGCTCAAATGCGGCTTc > 1:3293720/1‑69 (MQ=255) aaaCTGGCCTGCTGTTATCGGCAAACGCAACCCCAGTTCCGCCATCGTCa > 1:3098310/1‑50 (MQ=255) aaaCTGGCCTGCTGTTATCGGCAAACGCAACCCCAGTTCCGCCATCGTCAGCCGCTCAAATGCGGCTTc > 1:1694712/1‑69 (MQ=255) cTGGCCTGCTGTTATCGGCAAACGCAGCCCCAGTTCCGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCg > 1:1422185/1‑71 (MQ=255) ctgctgTTATCGGCAAACGCAGCCCCAGTTCCGCCATCGTCAGCCGCTCa < 1:2775325/50‑1 (MQ=255) tgctgTTATCGGCAAACGCAACCCCAGTTCCGCCATCGTCAGCCGCTCaa < 1:605929/50‑1 (MQ=255) gctgTTATCGGCAAACGCAGCCCCAGTTCCGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGtt < 1:2535670/66‑1 (MQ=255) ctgTTATCGGCAAACGCAGCCCCAGTTCCGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGt < 1:1667902/69‑1 (MQ=255) ttATCGGCAAACGCAGCCCCAGTTCCGCCATCGTCAGCCGCTCAAATGCg < 1:4018211/50‑1 (MQ=255) ttATCGGCAAACGCAACCCCAGTTCCGCCATCGTCAGCCGCTCAAATGCGGc > 1:123472/1‑52 (MQ=255) aTCGGCAAACGCAACCCCAGTTCCGCCATCGTCAGCCGCTCaa > 1:4083304/1‑43 (MQ=255) aTCGGCAAACGCAACCCCAGTTCCGCCATCGTCAGCCGCTCaa > 1:1260950/1‑43 (MQ=255) aTCGGCAAACGCAACCCCAGTTCCGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGttt > 1:3624114/1‑61 (MQ=255) aTCGGCAAACGCAACCCCAGTTCCGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTg > 1:713287/1‑71 (MQ=255) aTCGGCAAACGCAACCCCAGTTCCGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTg > 1:4019444/1‑71 (MQ=255) aTCGGCAAACGCAACCCCAGTTCCGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTg > 1:1861523/1‑71 (MQ=255) aTCGGCAAACGCAACCCCAGTTCCGCCATCGTCAGCCGCTCAAATGCCGCTTCCAg > 1:528576/1‑56 (MQ=39) tCGGCAAACGCAGCCCCAGTTCCGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTg < 1:2599361/70‑1 (MQ=255) ggCAAACGCAGCCCCAGTTCCGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCa < 1:2512172/70‑1 (MQ=255) cAAACGCAACCCCAGTTCCGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGc > 1:1738966/1‑52 (MQ=255) aaaCGCAACCCCAGTTCCGCCATCGTCAGCCGCTCa < 1:2387532/36‑1 (MQ=255) cGCAGCCCCAGTTCCGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGc > 1:3927506/1‑48 (MQ=255) cAGCCCCAGTTCCGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTc < 1:3365385/71‑1 (MQ=255) | CCAGAGAAGTTATCGTCATAAAAGTGCTGCCAGACACCGTAAAACTGGCCTGCTGTTATCGGCAAACGCAGCCCCAGTTCCGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTC > minE/1334545‑1334683 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |