| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,358,189 | G→T | 10.7% | S311R (AGC→AGA) | yehB ← | predicted outer membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,358,189 | 0 | G | T | 10.7% | 178.9 / 4.7 | 55 | S311R (AGC→AGA) | yehB | predicted outer membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (20/29); new base T (3/3); total (23/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTTGCAGCATATTTGGCACCGCTGCATAAGGCACCAGATAGGTGGTTACCGAGCCGTCCGCCTCTTTCACGCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGAACGGGCCAGGAGGAACC > minE/1358119‑1358258 | gTTGCAGCATATTTGGCACCGCTGCATAAGGCACCAGATAGGTGGTTACCGAGCCGTCCGCCTCTTTCACg > 1:1066621/1‑71 (MQ=255)gTTGCAGCATATTTGGCACCGCTGCATAAGGCACCAGATAGGTGGTTACCGAGCCGTCCGCCTCTTTCACg > 1:3946783/1‑71 (MQ=255) cagcaTATTTGGCACCGCTGCATAAGGCACCAGATAGGTGGTTACCGAGCCGTCCGCCTCTTTCACGCTGa < 1:3572516/71‑1 (MQ=255) gcaTATTTGGCACCGCTGCATAAGGCACCAGATAGGTGGTTACCGAGCCGTCCGCCTCTTTCACGCTGACa < 1:1155151/71‑1 (MQ=255) gcaTATTTGGCACCGCTGCATAAGGCACCAGATAGGTGGTTACCGAGCCGTCCGCCTCTTTCACGCTGACa < 1:3749805/71‑1 (MQ=255) tGGCACCGCTGCATAAGGCACCAGATAGGTGGTTACCGAGCCGTCCGCCTCTTTCACGCTGACATc > 1:197242/1‑66 (MQ=255) tGGCACCGCTGCATAAGGCACCAGATAGGTGGTTACCGAGCCGTCCGCCTCTTTCACGCTGACATCAAGa < 1:473836/70‑1 (MQ=255) aCCGCTGCATAAGGCACCAGATAGGTGGTTACCGAGCCGTCCGCCTCTTTCACGCTGACATCAAGATCTGc < 1:316650/71‑1 (MQ=255) aCCGCTGCATAAGGCACCAGATAGGTGGTTACCGAGCCGTCCGCCTCTTTCACGCTGACATCAAGATCTGc > 1:3047392/1‑71 (MQ=255) ccGCTGCATAAGGCACCAGATAGGTGGTTACCGAGCCGTCCGCCTCTTTCACGCTGACATCAAGATCTg < 1:1746270/69‑1 (MQ=255) gCTGCATAAGGCACCAGATAGGTGGTTACCGAGCCGTCCGCCTCTTTCACGCTGa > 1:581310/1‑55 (MQ=255) cTGCATAAGGCACCAGATAGGTGGTTACCGAGCCGTCCGCCTCTTTCACTCTGACATCAAGATCTGCTcca > 1:752372/1‑71 (MQ=255) cTGCATAAGGCACCAGATAGGTGGTTACCGAGCCGTCCGCCTCTTTCACGCTGACATCAAg < 1:2560840/61‑1 (MQ=255) cATAAGGCACCAGATAGGTGGTTACCGAGCCTTCCGCCTCTTTCACGCTGACATCAAGATCTGCTccac < 1:936378/69‑1 (MQ=255) cATAAGGCACCAGATAGGTGGTTACCGAGCCGTCCGCCTCTTTCACGCTGACATCAAGATCTGCTccacc < 1:2219587/70‑1 (MQ=255) cATAAGGCACCAGATAGGTGGTTACCGAGCCGTCCGCCTCTTTCACGCTGACATCAAGATCTGCTccac < 1:3855713/69‑1 (MQ=255) aGGCACCAGATAGGTGGTTACCGAGCCGTCCGCCTCTTTCACGCTGACATc < 1:2647077/51‑1 (MQ=255) aGGCACCAGATAGGTGGTTACCGAGCCGTCCGCCTCTTTCACGCTGACATCAAGAt > 1:2438771/1‑56 (MQ=255) aCCAGATAGGTGGTTACCGAGCCGTCCGCCTCTTTCACTCTGACATCAAGATCTg > 1:1271053/1‑55 (MQ=255) ccAGATAGGTGGTTACCGAGCCGTCCGCCTCTTTCACGCTGACATCAAGATCTGCTccacc < 1:1389868/61‑1 (MQ=255) cAGATAGGTGGTTACCGAGCCGTCCGCCTCTTTCACg < 1:1879226/37‑1 (MQ=255) gATAGGTGGTTACCGAGCCGTCCGCCTCTTTCACGCTGACATCAAg < 1:825813/46‑1 (MQ=255) gATAGGTGGTTACCGAGCCGTCCGCCGCTTTCACGCTGACATCAAGATCTGCTCCACCACCGGCCAACt > 1:2696352/1‑69 (MQ=255) tggtTACCGAGCCGTCCGCCTCTTTCACGCTGACATCAAg < 1:776050/40‑1 (MQ=255) tggtTACCGAGCCGTCCGCCTCTTTCACGCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCt < 1:2836164/71‑1 (MQ=255) ggtTACCGAGCCGTCCGCCTCTTTCACGCTGACATCAAGATCTGc < 1:1840594/45‑1 (MQ=255) gtTACCGAGCCGTCCGCCTCTTTCACGCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTg > 1:2628100/1‑70 (MQ=255) gtTACCGAGCCGTCCGCCTCTTTCACGCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGt < 1:2277863/71‑1 (MQ=255) tACCGAGCCGTCCGCCTCTTTCACGCTGACATCAAGATCTGCt > 1:1879513/1‑43 (MQ=255) aCCGAGCCGTCCGCCTCTTTCACGCTGACATCAAGa < 1:1643702/36‑1 (MQ=255) aCCGAGCCGTCCGCCTCTTTCACGCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAAt > 1:2425598/1‑71 (MQ=255) cGAGCCGTCCGCCTCTTTCACGCTGACATCAAGATc > 1:3473983/1‑36 (MQ=255) cGAGCCGTCCGCCTCTTTCACGCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATcg < 1:3353314/71‑1 (MQ=255) gAGCCGTCCGCCTCTTTCACGCTGACATCAAGATCTGCTc < 1:168934/40‑1 (MQ=255) gAGCCGTCCGCCTCTTTCACGCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCaa < 1:2723204/59‑1 (MQ=255) gAGCCGTCCGCCTCTTTCACGCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCaa < 1:4020911/59‑1 (MQ=255) aGCCGTCCGCCTCTTTCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATcgcg < 1:179673/71‑1 (MQ=255) gTCCGCCTCTTTCACGCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCaa < 1:2758002/54‑1 (MQ=255) cGCCTCTTTCACGCTGACATCAAGATCTGCTCCACCACCGGCCAAc > 1:1879574/1‑46 (MQ=255) cGCCTCTTTCACGCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGa > 1:2653491/1‑65 (MQ=255) cGCCTCTTTCACGCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGa > 1:2330014/1‑65 (MQ=255) cGCCTCTTTCACGCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGa > 1:1476923/1‑65 (MQ=255) cGCCTCTTTCACGCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGAACGGGc > 1:4051702/1‑71 (MQ=255) ccTCTTTCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGAACGGGc > 1:588987/1‑69 (MQ=255) ctctTTCACGCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGAACGGGCCag > 1:197820/1‑71 (MQ=255) ctctTTCACGCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGAACGGGCCag < 1:4095044/71‑1 (MQ=255) tttCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATc < 1:865280/48‑1 (MQ=255) tttCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATc < 1:3943705/48‑1 (MQ=255) tttCACGCTGACATCAAGATCTGCTCCACCACCGGCCaa > 1:310526/1‑39 (MQ=255) tttCACGCTGACATCAAGATCTGCTCCACCACCGGCCaa > 1:2632717/1‑39 (MQ=255) tttCACGCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTg < 1:2295198/50‑1 (MQ=255) ttCACGCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGAACGGGCCaggag > 1:478678/1‑70 (MQ=255) ttCACGCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGAACGGGCCa > 1:3999930/1‑66 (MQ=255) tCACGCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGAACGGGCCaggagg < 1:3967905/70‑1 (MQ=255) tCACGCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGAACGGGCCaggagg > 1:2005272/1‑70 (MQ=255) tCACGCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGAACGGGCCaggagg < 1:1722985/70‑1 (MQ=255) tCACGCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGAACGGGCCaggagg < 1:103451/70‑1 (MQ=255) cGCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGAACGGGCCAGGAGGAAcc < 1:434091/71‑1 (MQ=255) | GTTGCAGCATATTTGGCACCGCTGCATAAGGCACCAGATAGGTGGTTACCGAGCCGTCCGCCTCTTTCACGCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGAACGGGCCAGGAGGAACC > minE/1358119‑1358258 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |