| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,368,852 | T→C | 40.7% | D188G (GAT→GGT) | dusC ← | tRNA‑dihydrouridine synthase C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,368,852 | 0 | T | C | 40.7% | 31.9 / 70.0 | 59 | D188G (GAT→GGT) | dusC | tRNA‑dihydrouridine synthase C |
| Reads supporting (aligned to +/- strand): ref base T (20/15); new base C (8/16); total (28/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.33e-01 | |||||||||||
GTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCAC > minE/1368784‑1368919 | gttgCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATc < 1:786755/70‑1 (MQ=255)gttgCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCg > 1:2192236/1‑71 (MQ=255) gcgcgcTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCGTAATATTCAGCCGCTGGCGAATATCGcc < 1:1957775/70‑1 (MQ=255) gcgcgcTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGcc > 1:2062778/1‑70 (MQ=255) ccAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAatat > 1:1804086/1‑56 (MQ=255) ccAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGc > 1:3764979/1‑70 (MQ=255) cAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGc > 1:1467795/1‑65 (MQ=255) ccAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCAAGTCa > 1:2526650/1‑70 (MQ=255) aGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAata > 1:914188/1‑71 (MQ=255) gATTTCACCGTTGGCAATCACCGGAATATTCAGCCGATGGCGAATATCGCCAATCGCCTGCCAGTCAatat > 1:1247154/1‑71 (MQ=255) tttCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGc > 1:369568/1‑71 (MQ=255) tttCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGc < 1:1243800/71‑1 (MQ=255) ttCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTc > 1:3963688/1‑63 (MQ=255) gTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCcgcgc < 1:2898966/71‑1 (MQ=255) ttGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCg > 1:3413215/1‑39 (MQ=255) gCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGt < 1:1762085/70‑1 (MQ=255) gCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGt < 1:537431/70‑1 (MQ=255) tCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAAccc > 1:99243/1‑71 (MQ=255) cACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCt > 1:2748662/1‑71 (MQ=255) aCCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCt < 1:1138926/55‑1 (MQ=255) gAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTc < 1:3129602/43‑1 (MQ=255) gAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAata > 1:1299838/1‑47 (MQ=255) gAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGctct < 1:4114878/71‑1 (MQ=255) gAATATTCAGCCGCTGGCGAAAATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGctct < 1:4125291/71‑1 (MQ=255) gAATATTCAGCCGCTGGCGAAAATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGctct < 1:4129908/71‑1 (MQ=255) atatTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCaa > 1:2601446/1‑43 (MQ=255) tatTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTc < 1:311052/71‑1 (MQ=255) ttCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGt > 1:646995/1‑71 (MQ=255) ttCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGt > 1:611395/1‑71 (MQ=255) tCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAata > 1:3760441/1‑41 (MQ=255) cAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTcc > 1:851985/1‑71 (MQ=255) caccgcTGGCGAATACCGCattcaggaaggaaatgcgagtgatttcaccgtcaccggcacaaccgatccgc > 1:1725937‑M1/3‑19 (MQ=255) caccgcTGGCGAATACCGCattcaggaaggaaatgcgagtgatttcacc < 1:2766843‑M1/47‑31 (MQ=255) aGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATg > 1:3111066/1‑41 (MQ=255) aGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATg > 1:3130215/1‑41 (MQ=255) gCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTcctc < 1:2393672/71‑3 (MQ=255) accgCTGGCGAATACCGCattcaggaaggaaatgcgagtgatttcaccgtcaccggcacaaccgatccgc > 1:3846587‑M1/2‑18 (MQ=255) accgCTGGCGAATACCGCattcaggaaggaaatgcgagtgatttcaccgtcaccggcacaa > 1:1609139‑M1/2‑18 (MQ=255) accgCTGGCGAATACAGCattcaggaaggaaatgcgagtgatttcaccgtcaccggcacaa > 1:2722785‑M1/2‑18 (MQ=255) ccGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGcc < 1:3561759/71‑1 (MQ=255) ccGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGcc < 1:2820872/71‑1 (MQ=255) cGCTGGCGAATACCGCattcaggaaggaaatgcgagtgatttcaccgtcaccggcacaaccgatccgcca > 1:2174734‑M1/1‑16 (MQ=255) cGCTGGCGAATACCGCattcaggaaggaaatgcgagtga > 1:1131353‑M1/1‑16 (MQ=255) cGCTGGCGAATACCGCattcaggaaggaaatgcgagt < 1:3350481‑M1/37‑22 (MQ=255) gCTGGCGAATACCGCattcaggaaggaaatgcgagtgatttcaccgtcaccggcacaaccgatccgccaaa < 1:818966‑M1/71‑57 (MQ=255) gCTGGCGAATACCGCattcaggaaggaaatgcgagtgatttcaccgtcaccggcacaaccgatccgccaaa < 1:1901858‑M1/71‑57 (MQ=255) gCTGGCGAATACCGCattcaggaaggaaatgcgagtgatttcaccgtcaccggcacaaccgatccgccaaa < 1:1522625‑M1/71‑57 (MQ=255) gCTGGCGAATACCGCattcaggaaggaaatgcgagtgatttc < 1:1867202‑M1/42‑28 (MQ=255) cTGGCGAATACCGCattcaggaaggaaatgcgagtgatttcaccgtcaccggcacaaccgatccgccaaa > 1:3638509‑M1/1‑14 (MQ=255) cTGGCGAATACCGCattcaggaaggaaatgcgagtgatttcaccgtcaccggcacaaccgatccgccaaa > 1:754419‑M1/1‑14 (MQ=255) tGGCGAATACCGCattcaggaaggaaatgcgagtga < 1:1624554‑M1/36‑24 (MQ=255) tGGCGAATACCGCattcaggaaggaaatgcgagtga < 1:656680‑M1/36‑24 (MQ=255) gCGAATACCGCattcaggaaggaaatgcgagtgatttcaccgtcaccggcacaaccgatccgccaaaaaga < 1:3254188‑M1/71‑61 (MQ=255) gCGAATACCGCattcaggaaggaaatgcgagtgatttcaccgtcaccggcacaaccgatccgccaaaaa < 1:2312531‑M1/69‑59 (MQ=255) gCGAATACCGCattcaggaaggaaatgcgagtgatttcaccgtcaccggcacaaccgatccgccaaaaa < 1:3709041‑M1/69‑59 (MQ=255) gCGAATACCGCattcaggaaggaaatgcgagtgatttcaccgtcaccgg < 1:659449‑M1/49‑39 (MQ=255) cGAATACCGCattcaggaaggaaatgcgagtgatttcaccgtcaccggcacaaccgatccgccaaaaaga < 1:1995104‑M1/70‑61 (MQ=255) cGAATACCGCattcaggaaggaaatgcgagtgatttcaccgtcaccggcacaaccgatccgccaaaaa < 1:696912‑M1/68‑59 (MQ=255) cGAATACCGCattcaggaaggaaatgcgagtgatttcaccgtcaccggcacaaccgatccgccaaaaa < 1:79003‑M1/68‑59 (MQ=255) gAATACCGCattcaggaaggaaatgcgagtgatttcaccgtcaccggcacaaccga < 1:2697180‑M1/56‑48 (MQ=255) atatCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTg > 1:2998969/1‑47 (MQ=255) atatCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGc > 1:3329087/1‑48 (MQ=255) atacCGCattcaggaaggaaatgcgagtgatttcaccgtcaccggcacaaccgatccgccaaaaagaggcg < 1:937855‑M1/71‑65 (MQ=255) atacCGCattcaggaaggaaatgcgagtgatttcaccgtcaccggcacaaccgatc > 1:696642‑M1/1‑7 (MQ=255) tatCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCttt > 1:2872765/1‑52 (MQ=255) tatCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTc < 1:2781221/53‑1 (MQ=255) tatCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTc < 1:180762/53‑1 (MQ=255) tatCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGcaccac > 1:1372096/1‑70 (MQ=255) | GTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCAC > minE/1368784‑1368919 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |