Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 218,106 G→T 39.7% E31D (GAG→GAT yafF → conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE218,1060GT39.7% 215.5 / 152.2 161E31D (GAG→GATyafFconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (51/46);  new base T (38/26);  total (89/72)
Fisher's exact test for biased strand distribution p-value = 4.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

Only 100 of 167 total aligned reads displayed.

TAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGT  >  minE/218036‑218173
                                                                      |                                                                   
tAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATgag                                                                     <  1:843026/71‑1 (MQ=255)
  agaagaGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATgaga                                                                    <  1:3523578/70‑1 (MQ=255)
    aagaGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAgg                                                                 <  1:1545557/71‑1 (MQ=255)
    aagaGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAgg                                                                 <  1:716141/71‑1 (MQ=255)
     agagGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAgg                                                                 <  1:1554768/70‑1 (MQ=255)
       agGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTCGACGAATGATAAGGTAt                                                              >  1:530055/1‑71 (MQ=255)
       agGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTAt                                                              <  1:1766001/71‑1 (MQ=255)
       agGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTAt                                                              <  1:2711932/71‑1 (MQ=255)
         gAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTc                                                            <  1:1858237/71‑1 (MQ=255)
          aaaCGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTc                                                            >  1:2414334/1‑70 (MQ=255)
           aaCGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAgg                                                                 >  1:4120883/1‑64 (MQ=255)
           aaCGCAGAAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAgg                                                                 <  1:335349/64‑1 (MQ=255)
                agcagAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAg                                                     <  1:3959747/71‑1 (MQ=255)
                    gAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGt                                                  >  1:795900/1‑70 (MQ=255)
                    gAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGt                                                  >  1:3909818/1‑70 (MQ=255)
                    gAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGtt                                                 <  1:3020988/71‑1 (MQ=255)
                     aaTTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTc                                                            <  1:3529575/59‑1 (MQ=255)
                       ttattTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTa                                                               >  1:2049436/1‑54 (MQ=255)
                       ttattTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTa                                                               >  1:3325708/1‑54 (MQ=255)
                       ttattTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTAAAGGCAGGGtt                                                 <  1:2115687/68‑1 (MQ=255)
                       ttattTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTa                                                               >  1:428243/1‑54 (MQ=255)
                       ttattTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTc                                                            <  1:2369212/57‑1 (MQ=255)
                       ttattTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGtt                                                 >  1:2289413/1‑68 (MQ=255)
                        tattTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGa                                             >  1:3755574/1‑71 (MQ=255)
                         attTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAg                                                                  >  1:1490770/1‑49 (MQ=255)
                         attTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGa                                             <  1:1376734/70‑1 (MQ=255)
                         attTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGCTAAGa                                             <  1:1009832/70‑1 (MQ=255)
                          ttttCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTaa                                               <  1:1928564/67‑1 (MQ=255)
                          tttaCAGGGATAGCGCACATTGCTATTAATATTTTGACGAATGAGaact                                                                 >  1:3174574/1‑47 (MQ=37)
                           tttCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAgg                                                                 >  1:14288/1‑48 (MQ=255)
                            ttCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGt                                          >  1:896646/1‑70 (MQ=255)
                             tCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAggg                                                   >  1:2709848/1‑60 (MQ=255)
                              cAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTaa                                        <  1:2037064/70‑1 (MQ=255)
                              cAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGtt                                                 <  1:1082554/61‑1 (MQ=255)
                              cAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTaa                                        <  1:2182092/70‑1 (MQ=255)
                              cAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTa                                         <  1:1909966/69‑1 (MQ=255)
                               aGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAg                                                                  <  1:4058393/43‑1 (MQ=255)
                               aGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAgg                                                        <  1:2914731/53‑1 (MQ=255)
                                 ggATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTc                                                            <  1:762922/47‑1 (MQ=255)
                                 ggATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTc                                                            <  1:2658287/47‑1 (MQ=255)
                                    tACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTaa                                        <  1:1219158/64‑1 (MQ=255)
                                     aCGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGa                                 >  1:1944323/1‑70 (MQ=255)
                                     aCGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGa                                 >  1:168842/1‑70 (MQ=255)
                                      cGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTaa                                               >  1:894209/1‑55 (MQ=255)
                                      cGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGaa                                >  1:3809139/1‑70 (MQ=255)
                                      cGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTaa                                               >  1:2223755/1‑55 (MQ=255)
                                      cGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTaa                                        >  1:1518953/1‑62 (MQ=255)
                                      cGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTaa                                        >  1:2026056/1‑62 (MQ=255)
                                      cGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGaa                                <  1:1139031/70‑1 (MQ=255)
                                      cGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGaa                                >  1:3708275/1‑70 (MQ=255)
                                        gCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGAc                                            <  1:3929927/56‑1 (MQ=255)
                                        gCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAag                             <  1:1743619/71‑1 (MQ=255)
                                          acaTTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAagca                           <  1:238345/71‑1 (MQ=255)
                                           caTCGCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAagcag                          <  1:1212147/71‑1 (MQ=255)
                                            aTTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAagcagc                         >  1:1240525/1‑71 (MQ=255)
                                            aTTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAagcagc                         >  1:974694/1‑71 (MQ=255)
                                            aTTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGtt                                                 <  1:393297/47‑1 (MQ=255)
                                             ttGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAagcagc                         >  1:3886734/1‑70 (MQ=255)
                                             ttGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAagcagc                         >  1:1208351/1‑70 (MQ=255)
                                             tcgCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAagcagc                         >  1:1843493/3‑70 (MQ=255)
                                              tGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAgg                                                    >  1:1123139/1‑42 (MQ=255)
                                              tGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAag                             >  1:348068/1‑65 (MQ=255)
                                               gCTATTAATATTTTGACGAATGATAAGGTATTCaa                                                          >  1:2225364/1‑35 (MQ=255)
                                               gCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCAt                      >  1:1811614/1‑71 (MQ=255)
                                                cTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGAc                                            >  1:4006659/1‑48 (MQ=255)
                                                   ttAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTaa                                               <  1:2563745/42‑1 (MQ=255)
                                                   ttAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGt                                          <  1:1165712/47‑1 (MQ=255)
                                                   ttAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATgg                    <  1:38476/69‑1 (MQ=255)
                                                     aaTATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTaa                                               >  1:3218936/1‑40 (MQ=255)
                                                     aaTATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACg                                           <  1:1235907/44‑1 (MQ=255)
                                                     aaTATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAag                             >  1:2727963/1‑58 (MQ=255)
                                                     aaTATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGa                   >  1:509741/1‑68 (MQ=255)
                                                     aaTATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGa                   >  1:2444284/1‑68 (MQ=255)
                                                      atatTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAg                >  1:3181590/1‑70 (MQ=255)
                                                       tatTTTGACGAATGATAAGGTATTCAAGGCAGGGtt                                                 <  1:2291779/36‑1 (MQ=255)
                                                       tatTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGaaaa                              >  1:104092/1‑55 (MQ=255)
                                                       tatTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAg                                       <  1:2373044/46‑1 (MQ=255)
                                                       tatTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAATATGCGAAAAGCAGCCATGGACAGaa              <  1:647353/71‑1 (MQ=255)
                                                       tatTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGcc                        >  1:2048841/1‑61 (MQ=255)
                                                       tatTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGa               <  1:145670/70‑1 (MQ=255)
                                                        atTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGAc                  >  1:3642772/1‑66 (MQ=255)
                                                        atTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGaaa             >  1:2332996/1‑71 (MQ=255)
                                                         ttttGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGa               <  1:3254819/68‑1 (MQ=255)
                                                         ttttGACGAATGAGAATGTATTCAAGGCAGGGTTAAGACGt                                          <  1:3294372/41‑1 (MQ=255)
                                                         ttttGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGAc                                            >  1:775226/1‑39 (MQ=255)
                                                          tttGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGa               <  1:1430416/67‑1 (MQ=255)
                                                           ttaaCGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACt           <  1:2144779/67‑1 (MQ=255)
                                                           ttGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTaa                                        >  1:2946894/1‑41 (MQ=255)
                                                           ttGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAagcag                          >  1:68080/1‑55 (MQ=255)
                                                           ttGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAagcag                          >  1:899948/1‑55 (MQ=255)
                                                           ttGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAagcag                          >  1:221555/1‑55 (MQ=255)
                                                            tGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGa                   >  1:1444284/1‑61 (MQ=255)
                                                             aaCGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTatc        >  1:2302951/2‑69 (MQ=255)
                                                              aCGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTatc        <  1:4033557/70‑3 (MQ=255)
                                                                 aaTGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGc    <  1:1917859/71‑1 (MQ=255)
                                                                  aTGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCg   >  1:3207457/1‑71 (MQ=255)
                                                                   tGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAagcagc                         <  1:2897166/48‑1 (MQ=255)
                                                                   tGAGAAGGTATTAAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACt           >  1:2022421/1‑62 (MQ=255)
                                                                      gaAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCg                                  <  1:2609051/36‑1 (MQ=255)
                                                                      gaAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCg                                  <  1:939455/36‑1 (MQ=255)
                                                                      |                                                                   
TAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGT  >  minE/218036‑218173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: