| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,390,615 | A→G | 18.7% | Q249Q (CAA→CAG) | nfo → | endonuclease IV with intrinsic 3'‑5' exonuclease activity |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,390,615 | 0 | A | G | 18.7% | 175.6 / 36.2 | 80 | Q249Q (CAA→CAG) | nfo | endonuclease IV with intrinsic 3'‑5' exonuclease activity |
| Reads supporting (aligned to +/- strand): ref base A (30/35); new base G (6/9); total (36/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01 | |||||||||||
GCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCG > minE/1390545‑1390685 | gCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCaa < 1:645476/71‑1 (MQ=255) gcgTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGga > 1:1117587/1‑70 (MQ=255) gcgTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAgac < 1:1553782/71‑1 (MQ=255) aCCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGt < 1:3757260/71‑1 (MQ=255) aCCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGt < 1:2085752/71‑1 (MQ=255) gCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTc < 1:3238087/71‑1 (MQ=255) gCCATCATAGCCTCGGTGAAGGCAATATCGGGCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTc < 1:2590592/71‑1 (MQ=255) ccATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCg < 1:3588733/71‑1 (MQ=255) tcatAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCaa < 1:497728/55‑1 (MQ=255) atAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGga > 1:3107503/1‑55 (MQ=255) atAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAgacga > 1:1060787/1‑58 (MQ=255) atAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACgg > 1:381405/1‑70 (MQ=255) atAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACgg > 1:2307481/1‑70 (MQ=255) atAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACgg < 1:2069635/70‑1 (MQ=255) tAGCCTCGGTGAAGGCAATATCGGTCATGATGCTTTCCGCTGGATCATGCAAgacg < 1:3958388/56‑1 (MQ=255) aGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGa > 1:3254524/1‑65 (MQ=255) ccTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTc < 1:1955480/61‑1 (MQ=255) ccTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCAt < 1:3794228/69‑1 (MQ=255) cTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTc < 1:1684288/60‑1 (MQ=255) ggTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCa > 1:1175644/1‑64 (MQ=255) ggTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCt > 1:1866889/1‑71 (MQ=255) tGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGAcc < 1:1665315/50‑1 (MQ=255) gAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGAt > 1:2521716/1‑71 (MQ=255) aaGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAgacgac > 1:1200213/1‑47 (MQ=255) aaGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAgacgac > 1:2113851/1‑47 (MQ=255) ggCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCt > 1:3417377/1‑71 (MQ=255) gCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGt < 1:2624687/47‑1 (MQ=255) gCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTc > 1:3767952/1‑71 (MQ=255) aaTATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACgg < 1:4139565/53‑1 (MQ=255) aaTATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACgg < 1:3738454/53‑1 (MQ=255) aaTATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCg > 1:105877/1‑70 (MQ=255) aaTATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGa < 1:1249164/71‑1 (MQ=255) atatCGGTCATGATGCGTTCCGCTGGATCATGCAAg < 1:221349/36‑1 (MQ=255) atatCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTc < 1:1834054/47‑1 (MQ=255) atatCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGa > 1:3902796/1‑70 (MQ=255) tatCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGaaa < 1:1929967/71‑1 (MQ=255) tatCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGaaa > 1:3897926/1‑71 (MQ=255) tatCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGaaa > 1:2245248/1‑71 (MQ=255) tatCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGaaa > 1:1473654/1‑71 (MQ=255) tCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGa > 1:2918319/1‑60 (MQ=255) tCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAAc > 1:3285774/1‑70 (MQ=255) tCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAAc < 1:2399049/70‑1 (MQ=255) tCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAAc > 1:3841776/1‑70 (MQ=255) ggTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCa < 1:3296979/70‑1 (MQ=255) tCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTc < 1:3813151/50‑1 (MQ=255) cATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCaa > 1:3878099/1‑71 (MQ=255) cATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCaa > 1:2104545/1‑71 (MQ=255) atgatgCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAAc > 1:771757/1‑71 (MQ=255) atgCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCg < 1:4139049/71‑1 (MQ=255) atgCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCg > 1:227514/1‑71 (MQ=255) atgCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCg < 1:948327/71‑1 (MQ=255) tgCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCgg < 1:3169352/71‑1 (MQ=255) tgCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCg > 1:2553965/1‑70 (MQ=255) cGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGat < 1:1715520/71‑1 (MQ=255) gTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCt > 1:294473/1‑45 (MQ=255) gTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGata < 1:977069/71‑1 (MQ=255) ttCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCg < 1:2145915/66‑1 (MQ=255) ttCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGata > 1:449879/1‑70 (MQ=255) ccGCTGGATCATGCAGGACGACCTTTTCGACTGCATTc < 1:1858001/38‑1 (MQ=37) ccGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCt > 1:3629683/1‑71 (MQ=255) ccGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCt > 1:182310/1‑71 (MQ=255) cGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCt < 1:3455922/70‑1 (MQ=255) cTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAAc > 1:442998/1‑51 (MQ=255) gATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCag < 1:527727/71‑1 (MQ=255) tCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCagaa < 1:1588061/71‑1 (MQ=255) tCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCagaa < 1:2247148/71‑1 (MQ=255) aTGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGatat > 1:2720381/1‑59 (MQ=255) aTGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGatat > 1:2291608/1‑59 (MQ=255) aTGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAAcc > 1:1088036/1‑52 (MQ=255) aTGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCt < 1:1752999/61‑1 (MQ=255) aTGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCagaaga < 1:32295/71‑1 (MQ=255) aTGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCagaa < 1:3429077/69‑1 (MQ=255) tGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAagag < 1:3177270/71‑1 (MQ=255) tGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAagag < 1:686299/71‑1 (MQ=255) tGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATc < 1:194004/59‑1 (MQ=255) tGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAagag < 1:2360038/71‑1 (MQ=255) tGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAagag < 1:4143538/71‑1 (MQ=255) tGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAagag < 1:3461783/71‑1 (MQ=255) gCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCagaaga < 1:2674417/69‑1 (MQ=255) gCAAGACGACCGTTTCGCCGGCATTCCGCTGATCCTCGAAACCAt > 1:3815758/1‑45 (MQ=255) cAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCagaa > 1:2821025/1‑66 (MQ=255) cAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAgaga < 1:1905503/70‑1 (MQ=255) aGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCg > 1:3088039/1‑71 (MQ=255) aGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCg > 1:16968/1‑71 (MQ=255) | GCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCG > minE/1390545‑1390685 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |