| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,391,735 | T→C | 24.7% | K370E (AAA→GAA) | fruA ← | fused fructose‑specific PTS enzyme IIB'BC components |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,391,735 | 0 | T | C | 24.7% | 128.0 / 46.4 | 73 | K370E (AAA→GAA) | fruA | fused fructose‑specific PTS enzyme IIB'BC components |
| Reads supporting (aligned to +/- strand): ref base T (26/29); new base C (7/11); total (33/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.94e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01 | |||||||||||
GAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATCAGGATCGGTTT > minE/1391666‑1391804 | gAACCTCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTa < 1:3080924/71‑1 (MQ=255) ccGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTAcc > 1:3699283/1‑70 (MQ=255) ccGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCg < 1:384437/71‑1 (MQ=255) ccGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCg < 1:443106/71‑1 (MQ=255) cGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGa < 1:1428275/71‑1 (MQ=255) ttCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCTCCGATCAg < 1:1052977/71‑1 (MQ=255) tCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAgg > 1:3176837/1‑71 (MQ=255) cGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGAt > 1:167157/1‑66 (MQ=255) cGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAgg > 1:1370503/1‑70 (MQ=255) gCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTa < 1:1146796/71‑1 (MQ=255) cAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTa < 1:1939825/70‑1 (MQ=255) aGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGa < 1:204521/71‑1 (MQ=255) gTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGat < 1:164556/71‑1 (MQ=255) tCCCCATGGGCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGatc < 1:926662/71‑1 (MQ=255) cccATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCg < 1:598093/57‑1 (MQ=255) cccATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGatcat < 1:205375/71‑1 (MQ=255) aTGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGc > 1:3647111/1‑71 (MQ=255) gTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATAGc > 1:496278/1‑68 (MQ=255) gTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGATCATCGc > 1:2494588/1‑68 (MQ=255) tCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAga > 1:2779581/1‑71 (MQ=255) tCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAga > 1:1117237/1‑71 (MQ=255) cTGCGGCCAGTGAGTCAGCCCTTCGAGAATGCCCGCAACTGGTTCACCGATCAGGTAGATCATCGCCAgac > 1:3817218/1‑71 (MQ=255) tGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGatc < 1:2101642/60‑1 (MQ=255) cAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTa > 1:3670439/1‑43 (MQ=255) cAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGt < 1:3427440/53‑1 (MQ=255) aGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAgaccgac < 1:4002423/71‑1 (MQ=255) ccAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGacca > 1:3290093/1‑71 (MQ=255) ccAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGacca > 1:3285513/1‑71 (MQ=255) ccAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGacca > 1:2967843/1‑71 (MQ=255) gTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGAccacca < 1:1398400/71‑1 (MQ=255) gTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGAccacca < 1:619701/71‑1 (MQ=255) gTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGAccacca < 1:3187719/71‑1 (MQ=255) tGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGaccac > 1:2595212/1‑68 (MQ=255) gAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGat > 1:1838699/1‑49 (MQ=255) gAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGat > 1:3189556/1‑49 (MQ=255) gAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGa > 1:3169146/1‑48 (MQ=255) aGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGa < 1:2900723/71‑1 (MQ=255) aGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTgg > 1:2625078/1‑70 (MQ=255) aGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGa < 1:2373791/71‑1 (MQ=255) gCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGaa < 1:1637135/71‑1 (MQ=255) cTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTa < 1:3597245/37‑1 (MQ=255) cTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAg < 1:2617439/38‑1 (MQ=255) cTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAgaccgac < 1:1841050/55‑1 (MQ=255) cTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGaa < 1:308102/68‑1 (MQ=255) cTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAAtt < 1:1933753/71‑1 (MQ=255) tCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGa < 1:3265583/37‑1 (MQ=255) tCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAg > 1:3061802/1‑71 (MQ=255) cGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGaaa > 1:1826567/1‑66 (MQ=255) cGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAg > 1:2138020/1‑70 (MQ=255) cGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGc > 1:1561371/1‑71 (MQ=255) cGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGc > 1:2984885/1‑71 (MQ=255) cGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGc > 1:1553748/1‑71 (MQ=255) cGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGatcat > 1:598690/1‑40 (MQ=255) cGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAg < 1:337157/70‑1 (MQ=255) agaATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCgg < 1:1055288/71‑1 (MQ=255) agaATGCCAGCAACTGGTTCACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCgg < 1:4143893/71‑1 (MQ=255) gaATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAgaccgac > 1:2940641/1‑49 (MQ=255) gaATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGaccac < 1:2139990/52‑1 (MQ=255) aTGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAAt > 1:475509/1‑71 (MQ=255) tGCCAGCAACTGGTTTACCTATCAGGTAGATCATCGCCAgaccgac < 1:3737732/46‑1 (MQ=255) tGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGc < 1:2013622/37‑1 (MQ=255) tGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGaccac < 1:2990845/49‑1 (MQ=255) tGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGaccac < 1:467238/49‑1 (MQ=255) tGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTgg > 1:781664/1‑57 (MQ=255) gCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCa > 1:1691233/1‑38 (MQ=255) ccAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGAt > 1:2943981/1‑71 (MQ=255) ccAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGAt > 1:1391918/1‑71 (MQ=255) cAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATc < 1:2576145/71‑1 (MQ=255) cTGGTTTACCGATCAGGTAGATCATCGCCAgaccg < 1:348640/35‑1 (MQ=255) tGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATCAgg < 1:2025912/67‑1 (MQ=255) gTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATCAGGATCGGt > 1:3851760/1‑71 (MQ=255) gTTCACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATCAGGATCg < 1:4065136/69‑1 (MQ=255) gTTCACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATCAGGATCg < 1:2181933/69‑1 (MQ=255) tACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATCAGGATCGGttt < 1:1499428/70‑1 (MQ=255) | GAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATCAGGATCGGTTT > minE/1391666‑1391804 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |