| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,399,007 | C→T | 5.5% | A444V (GCC→GTC) | yeiQ → | predicted dehydrogenase, NAD‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,399,007 | 0 | C | T | 5.5% | 303.0 / 4.0 | 91 | A444V (GCC→GTC) | yeiQ | predicted dehydrogenase, NAD‑dependent |
| Reads supporting (aligned to +/- strand): ref base C (46/40); new base T (2/3); total (48/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTCGCGATCCGCTTAGCGATAAAATTCGCGAACTTGTTGCGGGCAGCAGCAGTGAACAACGCGTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGTGATGATCTGCCAGATAACCCGCATTTTGTGCAGGCCATC > minE/1398938‑1399074 | ttCGCGATCCGCTTAGCGATAAAATTCGCGAACTTGTTGCGGGCAGCAGCAGTGAACAACGCGTAACCGcc < 1:1566033/71‑1 (MQ=255) cgcgATCCGCTTAGCGATAAAATTCGCGAACTTGTTGCGGGCAGCAGCAGTGAACAACGCGTAACCGccc > 1:2833208/1‑70 (MQ=255) cgcgATCCGCTTAGCGATAAAATTCGCGAACTTGTTGCGGGCAGCAGCAGTGAACAACGCGTAACCGCCCt > 1:3508631/1‑71 (MQ=255) gATCCGCTTAGCGATAAAATTCGCGAACTTGTTGCGGGCAGCAGCAGTGAACAACGCGTAACCGCCCTGCt > 1:3850486/1‑71 (MQ=255) gATCCGCTTAGCGATAAAATTCGCGAACTTGTTGCGGGCAGCAGCAGTGAACAACGCGTAACCGCCCTGCt > 1:3740943/1‑71 (MQ=255) tCCGCTTAGCGATAAAATTCGCGAACTTGTTGCGGGCAGCAGCAGTGAACAACGCGTAACCGcc > 1:3732584/1‑64 (MQ=255) tCCGCTTAGCGATAAAATTCGCGAACTTGTTGCGGGCAGCAGCAGTGAACAACGCGTAACCGTCCTGCttt > 1:1949788/1‑71 (MQ=255) tCCGCTTAGCGATAAAATTCGCGAACTTGTTGCGGGCAGCAGCAGTGAACAACGCGTAACCGCCCTGCttt > 1:1633406/1‑71 (MQ=255) ttAGCGATAAAATTCGCGAACTTGTTGCGGGCAGCAGCAGTGAACAACGCGTAACCGc > 1:2816537/1‑58 (MQ=255) cGATAAAATTCGCGAACTTGTTGCGGGCAGCAGCAGTGAACAACGCGTAACCGCCCTGCTTTCCCTGCGTg < 1:377362/71‑1 (MQ=255) tAAAATTCGCGAACTTGTTGCGGGCAGCAGCAGTGAACAACGCGTAACCGCCCTGCTTTCCCTGCGTGAAg > 1:2379085/1‑71 (MQ=255) tAAAATTCGCGAACTTGTTGCGGGCAGCAGCAGTGAACAACGCGTAACCGCCCTGCTTTCCCTGCGTGAAg > 1:3794949/1‑71 (MQ=255) aaaaTTCGCGAACTTGTTGCGGGCAGCAGCAGTGAACAACGCGTAACCGCCCt < 1:608416/53‑1 (MQ=255) aaaaTTCGCGAACTTGTTGCGGGCAGCAGCAGTGAACAACGCGTAACCGCCCt > 1:3089428/1‑53 (MQ=255) aaaaTTCGCGAACTTGTTGCGGGCAGCAGCAGTGAACAACGCGTAACCGCCCTGCTTTccc > 1:3020302/1‑61 (MQ=255) aaaTTCGCGAACTTGTTGCGGGCAGCAGCAGTGAACAACGCGTAACCGCCCTGCTTTCCCTGCGTGAAGtt < 1:3997939/71‑1 (MQ=255) aaTTCGCGAACTTGTTGCGGGCAGCAGCAGTGAACAACGCGTAACCGTCCTGCTTTCCCTGCGTGAAGttt < 1:3326440/71‑1 (MQ=255) aaTTCGCGAACTTGTTGCGGGCAGCAGCAGTGAACAACGCGTAACCGCCCTGCTTTCCCTGCGTGAAGttt < 1:3846568/71‑1 (MQ=255) aTTCGCGAACTTGTTGCGGGCAGCAGCAGTGAACAACGCGTAACCGCCCTGCTTTCCCTGCGTGAAGtttt > 1:3825568/1‑71 (MQ=255) aTTCGCGAACTTGTTGCGGGCAGCAGCAGTGAACAACGCGTAACCGCCCTGCTTTCCCTGCGTGAAGtttt > 1:926467/1‑71 (MQ=255) aTTCGCGAACTTGTTGCGGGCAGCAGCAGTGAACAACGCGTAACCGCCCAGCTTTCCCTGCGTGAAGttt < 1:857204/70‑1 (MQ=255) ttCGCGAACTTGTTGCGGGCAGCAGCAGTGAACAACGCGTAACCGCCCTGCTTTCCCTGCGTGaa > 1:1287083/1‑65 (MQ=255) cgcgAACTTGTTGCGGGCAGCAGCAGTGAACAACGCGTAACCGTCCTGCTTTCCCTGCGTGAAGTTTTCg < 1:1987960/70‑1 (MQ=255) gcgAACTTGTTGCGGGCAGCAGCAGTGAACAACGCGTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGt < 1:1875739/71‑1 (MQ=255) gcgAACTTGTTGCGGGCAGCAGCAGTGAACAACGCGTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGt < 1:3554290/71‑1 (MQ=255) cgAACTTGTTGCGGTCAGCAGCAGTGAACAACGCGTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGtg < 1:2775891/71‑1 (MQ=255) aaCTTGTTGCGGGCAGCAGCAGTGAACAACGCGTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGtgat > 1:2148958/1‑71 (MQ=255) aCTTGTTGCGGGCAGCAGCAGTGAACAACGCGTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGtgatg < 1:3186830/71‑1 (MQ=255) aCTTGTTGCGGGCAGCAGCAGTGAACAACGCGTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGtgatg < 1:2030126/71‑1 (MQ=255) aCTTGTTGCGGGCAGCAGCAGTGAACAACGCGTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGtgatg < 1:2417871/71‑1 (MQ=255) aCTTGTTGCGGGCAGCAGCAGTGAACAACGCGTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGtgat > 1:2917368/1‑70 (MQ=255) ttgttgCGGGCAGCAGCAGTGAACAACGCGTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGtgatg < 1:3641927/69‑1 (MQ=255) ttgttgCGGGCAGCAGCAGTGAACAACGCGTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGtgatg < 1:23224/69‑1 (MQ=255) tgttgCGGGCAGCAGCAGTGAACAACGCGTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGTGATGATc > 1:997349/1‑71 (MQ=255) gttgCGGGCAGCAGCAGTGAACAACGCGTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGTGATGATCt > 1:2785056/1‑71 (MQ=255) tgCGGGCAGCAGCAGTGAACAACGCGTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGtga > 1:3312961/1‑63 (MQ=255) tgCGGGCAGCAGCAGTGAACAACGCGTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGTGATGATCTg < 1:328445/70‑1 (MQ=255) tgCGGGCAGAAGCAGTGAACAACGCGTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGtga > 1:3783200/1‑63 (MQ=255) gCGGGCAGCAGCAGTGAACAACGCGTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGtgat > 1:306565/1‑63 (MQ=255) gCGGGCAGCAGCAGTGAACAACGCGTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGTGATGATCTGc > 1:2349766/1‑70 (MQ=255) gcagcagcaGTGAACAACGCGTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGTGATGATCTGCCAGa > 1:1465466/1‑70 (MQ=255) cagcagcagTGAACAACGCGTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGTGATGATc > 1:223902/1‑62 (MQ=255) cgcagcagTGAACAACGCGTAACCGTCCTGCTTTCCCTGCGTGAAGTTTTCGGTGATGATCTGCCAGATaa > 1:2855024/2‑71 (MQ=255) cagcagTGAACAACGCGTAACCGTCCTGCTTTCCCTGCGTGAAGTTTTCGGTGATGATCTGCCAGa < 1:2434541/66‑1 (MQ=255) gcagTGAACAACGCGTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGtga > 1:2072771/1‑52 (MQ=255) agTGAACAACGCGTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGtgat > 1:2799952/1‑51 (MQ=255) gTGAACAACGCGTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGTGATGATCTGCCAGATAACCCGCAt < 1:103657/71‑1 (MQ=255) tGAACAACGCGTAACCGCCCTGCTTTCCCTGCGTGa < 1:1114048/36‑1 (MQ=255) tGAACAACGCGTAACCGCCCTGCTTTCCCTGCGTGa < 1:1229823/36‑1 (MQ=255) tGAACAACGCGTAACCGCCCTGCTTTCCCTGCGTGAAGtttt < 1:1849396/42‑1 (MQ=255) tGAACAACGCGTAACCGCCCTGCTTTCCCTGCGTGAAGtttt < 1:2287336/42‑1 (MQ=255) tGAACAACGCGTAACCGCCCTGCTTTCCCTGCGTGAAGttt < 1:2448248/41‑1 (MQ=255) tGAACAACGCGTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGtgatg < 1:2568240/50‑1 (MQ=255) tGAACAACGCGTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGt < 1:1065916/46‑1 (MQ=255) tGAACAACGCGTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGt < 1:2647328/46‑1 (MQ=255) tGAACAACGCGTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGTGGTGATCTGCCAGATAACCCGCAtt < 1:117797/71‑1 (MQ=255) gAACAACGCGTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGTGATGATCTGCCAGATAACCCGCAttt > 1:1854479/1‑71 (MQ=255) aacaacGCGTAACCGCCCTGCTTTCCCTGCGTGAAg > 1:3953726/1‑36 (MQ=255) aacaacGCGTAACCGCCCTGCTTTCCCTGCGTGAAGt > 1:2281713/1‑37 (MQ=255) aacaacGCGTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGTGATGATCTGCCAGATAACCCGCAtttt > 1:3847025/1‑71 (MQ=255) aacaacGCGTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGTGATGATCTGCCAGATAACCCGCAtttt < 1:3853650/71‑1 (MQ=255) aacaacGCGTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGTGATGATCTGCCAGATAACCCGCAtttt > 1:3814008/1‑71 (MQ=255) acaacGCGTAACCGCCCTGCTTTCCCTGCGTGAAGtttt < 1:3416276/39‑1 (MQ=255) acaacGCGTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGTGATGATCTGCCAGATAACCCGCAtt > 1:2753849/1‑68 (MQ=255) caacGCGTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCg > 1:1093888/1‑40 (MQ=255) caacGCGTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCg > 1:1832313/1‑40 (MQ=255) caacGCGTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGt < 1:821082/42‑1 (MQ=255) caacGCGTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGTGATGATCTGCCAGATAACCCGCATTTtgt < 1:3933544/71‑1 (MQ=255) caacGCGTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGTGATGATCTGCCAGATAACCCGCATTTtgt > 1:3820532/1‑71 (MQ=255) aacGCGTAACCGCCCTGCTTTCCCTGCGTGAAGtttt < 1:2237481/37‑1 (MQ=255) aacGCGTAACCGCCCTGCTTTCCCTGCGTGAAGtttt < 1:1272667/37‑1 (MQ=255) aacGCGTAACCGCCCTGCTTTCCCTGCGTGAAGtttt < 1:2610257/37‑1 (MQ=255) aacGCGTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGTGATGATCTGCCAGATAACCCGCATTTtgtg < 1:540981/71‑1 (MQ=255) aacGCGTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGTGATGATCTGCCAGATAACCCGCATTTtgtg < 1:3935201/71‑1 (MQ=255) aacGCGTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGTGATGATCTGCCAGATAACCCGCATTTtgtg < 1:2216301/71‑1 (MQ=255) acGCGTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGTGATGATCTGCCAGATAACCCGCATTTtgtg > 1:809189/1‑70 (MQ=255) acGCGTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGTGATGATCTGCCAGATAACCCGCATTTtgtg > 1:3217031/1‑70 (MQ=255) cgcgTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGtga > 1:259975/1‑41 (MQ=255) cgcgTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGTGATGATCTGCCAGATAACCCGCATTTtgtg > 1:1995184/1‑69 (MQ=255) cgcgTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGTGATGATCTGCCAGATAACCCGCATTTtg > 1:850997/1‑67 (MQ=255) gcgTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGt < 1:2782013/38‑1 (MQ=255) gcgTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGt < 1:2444846/38‑1 (MQ=255) gcgTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGTGATGATCTGCCAGATAACCCGCAtt < 1:860892/63‑1 (MQ=255) gcgTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGTGATGATCTGCCAGATAACCCGCATTTtgtg > 1:894374/1‑68 (MQ=255) gcgTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGTGATGATCTGCCAGATAACCCGCATTTTGTGCAg < 1:1626559/71‑1 (MQ=255) cgTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGTGATGATCTGCCAGATAACCCGCATTTTGTGCAgg > 1:3806821/1‑71 (MQ=255) cgTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGTGATGATCTGCCAGATAACCCGCATTTTGTGCAgg > 1:2158589/1‑71 (MQ=255) gTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGTGATGATCTGCCAGATAACCCGCATTTTGTGCAGGc > 1:147928/1‑71 (MQ=255) aCCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGtga > 1:394557/1‑35 (MQ=255) aCCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGTgatgat > 1:1299014/1‑39 (MQ=255) aCCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGTGATGATCTGCCAGATAACCCGCATTTTGTGCAGGCCAt > 1:840460/1‑71 (MQ=255) aCCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGTGATGATCTGACAGATAAc > 1:990530/1‑51 (MQ=255) aCCGCCCTGCTATCCCTGCGTGAAGTTTTCGGTgatgat > 1:2761280/1‑39 (MQ=255) ccGCCCTGCTTTCCCTGCGTGAAGTTTTCGGTGATGATCTGCCAGATAACCCGCATTTTGTGCAGGCCATc < 1:2148350/71‑1 (MQ=255) cGCCCTGCTTTCCCTGCGTGAAGTTTTCGGTGATGATCTGCCAGATAACCCGCATTTtgtg < 1:2320105/61‑1 (MQ=255) | TTCGCGATCCGCTTAGCGATAAAATTCGCGAACTTGTTGCGGGCAGCAGCAGTGAACAACGCGTAACCGCCCTGCTTTCCCTGCGTGAAGTTTTCGGTGATGATCTGCCAGATAACCCGCATTTTGTGCAGGCCATC > minE/1398938‑1399074 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |