Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,439,300 G→A 6.2% Y334Y (TAC→TAT ompC ← outer membrane porin protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,439,3000GA 6.2% 415.9 / 10.4 128Y334Y (TAC→TATompCouter membrane porin protein C
Reads supporting (aligned to +/- strand):  ref base G (57/63);  new base A (4/4);  total (61/67)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 131 total aligned reads displayed.

TGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGT  >  minE/1439230‑1439370
                                                                      |                                                                      
tGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCGGGTTGATTTTGTAGTCAACg                                                                        >  1:3811482/1‑71 (MQ=255)
 gTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACg                                                                        <  1:1144506/70‑1 (MQ=255)
  ttATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCATCGt                                                                       <  1:2493865/70‑1 (MQ=255)
  ttATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGt                                                                       <  1:3662189/70‑1 (MQ=255)
  ttATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGt                                                                       <  1:2093710/70‑1 (MQ=255)
  ttATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGt                                                                       <  1:333143/70‑1 (MQ=255)
  ttATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGt                                                                       <  1:420598/70‑1 (MQ=255)
  ttATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTa                                                                      <  1:3046581/71‑1 (MQ=255)
  ttATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTa                                                                      <  1:1183363/71‑1 (MQ=255)
    aTCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAgg                                                                    >  1:1113608/1‑71 (MQ=255)
    aTCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAgg                                                                    >  1:4014651/1‑71 (MQ=255)
     tCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGt                                                                   >  1:1299931/1‑71 (MQ=255)
       aGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTgg                                                                 <  1:3892708/71‑1 (MQ=255)
         tgtTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGa                                                                >  1:2302896/1‑70 (MQ=255)
         tgtTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGa                                                                >  1:726094/1‑70 (MQ=255)
           ttGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTg                                                                  <  1:1328128/66‑1 (MQ=255)
           ttGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAt                                                             >  1:1899211/1‑71 (MQ=255)
           ttGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAt                                                             >  1:2407113/1‑71 (MQ=255)
             gATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATg                                                            <  1:3537495/70‑1 (MQ=255)
                 ccAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGtttt                                                        <  1:2742389/70‑1 (MQ=255)
                  cAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttg                                                      <  1:3748841/71‑1 (MQ=255)
                     cGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgttg                                                   <  1:2411768/71‑1 (MQ=255)
                      gTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgttg                                                   >  1:4099008/1‑70 (MQ=255)
                      gTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgttg                                                   >  1:3870815/1‑70 (MQ=255)
                       tCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGt                                                                       <  1:2717181/49‑1 (MQ=255)
                       tCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTa                                                                      <  1:2567151/50‑1 (MQ=255)
                       tCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGttttt                                                       <  1:2866376/65‑1 (MQ=255)
                       tCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGaa                                                 <  1:3072708/71‑1 (MQ=255)
                        cACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGa                                                                <  1:2198893/55‑1 (MQ=255)
                        cACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAag                                                >  1:757978/1‑71 (MQ=255)
                         aCGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTa                                                                      >  1:972203/1‑48 (MQ=255)
                         aCGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACa                                                              >  1:3881837/1‑56 (MQ=255)
                         aCGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACa                                                              >  1:481327/1‑56 (MQ=255)
                         aCGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgtt                                                    >  1:3223453/1‑66 (MQ=255)
                         aCGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttg                                                      >  1:2949743/1‑64 (MQ=255)
                         aCGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGaa                                                 >  1:2331440/1‑69 (MQ=255)
                         aCGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGaa                                                 >  1:1853380/1‑69 (MQ=255)
                         aCGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGaa                                                 >  1:2192452/1‑69 (MQ=255)
                         aCGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGaa                                                 >  1:1843073/1‑69 (MQ=255)
                         aCGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagt                                               <  1:3106493/71‑1 (MQ=255)
                         aCGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAag                                                <  1:173355/70‑1 (MQ=255)
                           gAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagtag                                             <  1:1455832/71‑1 (MQ=255)
                           gAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagtag                                             <  1:1143437/71‑1 (MQ=255)
                            aGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGa                                                  <  1:2846807/65‑1 (MQ=255)
                                aaCTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGtttt                                                        >  1:1045058/1‑55 (MQ=255)
                                aaCTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtagg                                         >  1:2236668/1‑70 (MQ=255)
                                aaCTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtagg                                         >  1:1348598/1‑70 (MQ=255)
                                  cTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGa                                                  <  1:982141/59‑1 (MQ=255)
                                  cTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGa                                                  <  1:2689910/59‑1 (MQ=255)
                                     gTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGa                                                  <  1:3693229/56‑1 (MQ=255)
                                     gTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGa                                                  <  1:1860781/56‑1 (MQ=255)
                                     gTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCAc                                   >  1:643447/1‑71 (MQ=255)
                                        gtcgtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtagg                                         >  1:2449834/1‑62 (MQ=255)
                                         tcgtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgttg                                                   <  1:132814/51‑1 (MQ=255)
                                         tcgtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAag                                                <  1:1090898/54‑1 (MQ=255)
                                          catcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaat                               <  1:1463257/68‑2 (MQ=17)
                                          catcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaat                               <  1:2924583/68‑2 (MQ=17)
                                           gtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacat                             >  1:973159/1‑71 (MQ=255)
                                           gtcCAGCAGGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaatat                             >  1:1123461/1‑71 (MQ=18)
                                           gtcCAGCAGGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaatat                             >  1:1640258/1‑71 (MQ=18)
                                           gtcCAGCAGGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaata                              <  1:2530049/70‑3 (MQ=18)
                                           gtcCAGCAGGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaata                              <  1:2054752/70‑3 (MQ=18)
                                             ccAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGtttt                                                        >  1:793221/1‑42 (MQ=255)
                                              cagcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAag                                                >  1:1724234/1‑49 (MQ=255)
                                                gcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagtagt                                            <  1:259653/51‑1 (MQ=255)
                                                    gTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgttg                                                   <  1:3174141/40‑1 (MQ=255)
                                                    gTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagtagt                                            <  1:717467/47‑1 (MQ=255)
                                                    gTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagtag                                             <  1:757823/46‑1 (MQ=255)
                                                    gTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaac                               <  1:3488174/60‑1 (MQ=255)
                                                    gTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAtt                    <  1:3667023/71‑1 (MQ=255)
                                                    gTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGaaa                                                <  1:1563660/43‑2 (MQ=18)
                                                     ttGATTTTGTAGTCAACGTAGGTGGACATGTTTttgttg                                                   <  1:3935708/39‑1 (MQ=255)
                                                     ttGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagtagt                                            <  1:2067705/46‑1 (MQ=255)
                                                     ttGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagtagt                                            <  1:1291669/46‑1 (MQ=255)
                                                      tGATTTTGTAGTCAACGTAGGTGGACATGTTTttgt                                                     >  1:725818/1‑36 (MQ=255)
                                                      tGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagta                                              >  1:3075523/1‑43 (MQ=255)
                                                      tGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAgtagta                                           >  1:2249534/1‑46 (MQ=255)
                                                      tGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggta                                       >  1:1740684/1‑50 (MQ=255)
                                                      tGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt                   >  1:3466632/1‑70 (MQ=255)
                                                      tGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt                   >  1:3326456/1‑70 (MQ=255)
                                                      tGATTTTGTAATCAACATAGGTGGACATGTTTttgtt                                                    >  1:1525115/1‑37 (MQ=14)
                                                       gATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGaa                                                 >  1:4142818/1‑39 (MQ=255)
                                                       gATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAag                                                <  1:2574066/40‑1 (MQ=255)
                                                       gATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACCAATATCAACGTAttta                  <  1:2795632/70‑2 (MQ=17)
                                                        aTTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAg                <  1:3713999/71‑1 (MQ=255)
                                                             gTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGat             <  1:2152914/69‑1 (MQ=255)
                                                                gTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCt         <  1:61310/70‑1 (MQ=255)
                                                                gTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCt         <  1:1263415/70‑1 (MQ=255)
                                                                gTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCt         <  1:2311225/70‑1 (MQ=255)
                                                                 tCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAtt                    <  1:1438435/58‑1 (MQ=255)
                                                                 tCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtc       <  1:3228435/71‑1 (MQ=255)
                                                                  cAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCtt        >  1:2219639/1‑69 (MQ=255)
                                                                   aaCGTAGGTGGACATGTTTTTTTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtc       >  1:3473268/1‑69 (MQ=255)
                                                                   aaCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGatat           >  1:3354198/1‑65 (MQ=255)
                                                                   aaCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgt     >  1:627788/1‑71 (MQ=255)
                                                                   aaCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgt     >  1:2702276/1‑71 (MQ=255)
                                                                   aaCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgt     >  1:2967297/1‑71 (MQ=255)
                                                                   aaCGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaa                                >  1:3953001/1‑44 (MQ=11)
                                                                   aaCGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaa                                >  1:1669437/1‑44 (MQ=11)
                                                                      gTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtcgt  <  1:2832163/71‑1 (MQ=255)
                                                                      |                                                                      
TGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGT  >  minE/1439230‑1439370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: